Difference between revisions of "Biomod/2013/Sendai"

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<img src="http://openwetware.org/images/3/3c/%E5%90%8D%E7%A7%B0%E6%9C%AA%E8%A8%AD%E5%AE%9A-4.jpg" width="100%" height="100%">
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<br><div class="title">Egg-type initiator and<br>Chain-reactive burst</div>
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</div>
  
    <header>
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   <section role="main">
 
 
        <nav>     
 
          <div>
 
                <a href="#"  class="whiteSendai">Blog</a>
 
                <a href="#"  class="whiteSendai">Twitter</a>
 
                <a href="#"  class="whiteSendai">Facebook</a>
 
               
 
            </div>
 
            <a href="http://openwetware.org/wiki/Biomod/2013/Sendai" class="whiteSendai">Top</a>
 
            <a href="http://openwetware.org/wiki/Biomod/2013/Sendai/project" class="whiteSendai">Project</a>
 
            <a href="http://openwetware.org/wiki/Biomod/2013/Sendai/design" class="whiteSendai">Design</a>
 
            <a href="http://openwetware.org/wiki/Biomod/2013/Sendai/calcuation" class="whiteSendai">Calculation</a>
 
            <a href="http://openwetware.org/wiki/Biomod/2013/Sendai/experiment" class="whiteSendai">Experiment</a>
 
<a href="http://openwetware.org/wiki/Biomod/2013" class="whiteSendai" style="float:right;"><img src="http://openwetware.org/images/6/6e/Biomod-logo.jpg"
 
                                              width="75" height="75" alt="Biomod2013" border="0"></a><br>
 
            <a href="http://openwetware.org/wiki/Biomod/2013/Sendai/protocol" class="whiteSendai">Protocol</a>    
 
            <a href="http://openwetware.org/wiki/Biomod/2013/Sendai/future" class="whiteSendai">Future</a>
 
            <a href="http://openwetware.org/wiki/Biomod/2013/Sendai/member" class="whiteSendai">Member</a>
 
            <a href="http://openwetware.org/wiki/Biomod/2013/Sendai/sponsor" class="whiteSendai">Sponsor</a>
 
            </nav>
 
            <a href="http://openwetware.org/wiki/Biomod/2013/Sendai"><h1 style="color:white;" ><b>Biomod<span>2013<br>&emsp; Team</span>Sendai</b></h1></a>
 
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             <h3>Abstract</h3>
 
             <h3>Abstract</h3>
 
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<!--日本語隠すここから-->
            <p>私達のプロジェクトはトリガー入力による連鎖的分子放出システムの構築を目指している。 既存のDDSでは薬剤放出のタイミングの制御が困難な事、一度の入力に対して一定の出力しか得られない事が課題である。</br>
+
<!--
 +
          <p>私達のプロジェクトはトリガー入力による連鎖的分子放出システムの構築を目指している。 既存のDDSでは薬剤放出のタイミングの制御が困難な事、一度の入力に対して一定の出力しか得られない事が課題である。</br>
 
この問題を克服する為、私達はある条件でトリガーDNAを放出するシステムと、その放出を起点に連鎖的にリポソームが割れる出力増幅システムの2つを構築する。</br>
 
この問題を克服する為、私達はある条件でトリガーDNAを放出するシステムと、その放出を起点に連鎖的にリポソームが割れる出力増幅システムの2つを構築する。</br>
 
1つ目のシステムでは、トリガーを内在したアルギン酸ゲル膜を作り、温度の上昇に伴い自発的なゲルの融解とトリガーの放出が行われる。2つ目のシステムは、リポソーム表面にトリガーをハイブリし物理的な負荷をかけ、リポソームを破壊する。破壊されるリポソームは新たなトリガーを含んでおり、放出されたトリガーは連鎖的に周囲のリポソームを破壊する。</br>
 
1つ目のシステムでは、トリガーを内在したアルギン酸ゲル膜を作り、温度の上昇に伴い自発的なゲルの融解とトリガーの放出が行われる。2つ目のシステムは、リポソーム表面にトリガーをハイブリし物理的な負荷をかけ、リポソームを破壊する。破壊されるリポソームは新たなトリガーを含んでおり、放出されたトリガーは連鎖的に周囲のリポソームを破壊する。</br>
 
本システムの実現により入力のタイミングが制御可能かつ一度の入力で大量の薬剤を出力できるDDSの創出等への応用が期待できる。</br>
 
本システムの実現により入力のタイミングが制御可能かつ一度の入力で大量の薬剤を出力できるDDSの創出等への応用が期待できる。</br>
Our project aims at the construction of chain of molecules-releasing system by the trigger DNA input. They are problems in previous DDS(Drag Delivery System) that the control of timing releases trigger is difficult and only constant output can get from one time input.</br>
 
So, we created 2 systems to overcome these problems. The one is that releases trigger DNA on a certain condition. The other one is that increasing output by chain reaction of destroying liposome which starts from release of trigger.</br>
 
At the first system, we make alginate hydrogel membrane including trigger, then voluntary dissolution of hydrogel and release of trigger happens by increasing temperature. At the second system, we destroy liposome by hybridizing trigger DNA to the surface of liposome. The liposome which is destroyed by trigger includes new trigger DNA so released trigger destroys neighboring liposome as chain reaction.</br>
 
By creating these systems, it is expectable that creation of DDS which can control the timing of input and get a lot of output with one time input as application.</br>
 
  
            </p>
+
-->
 +
Our project aims to construct a chain-reactive molecule-releasing system in a spontaneous manner. Delivering drugs at appropriate places and controlling the quantity of released drug is very important to develop effective DDS. Our system makes these possible.<br>
 +
The system consists of two sub-systems: “egg-type initiator” and “chain-reactive burst”. <br>
 +
The “egg-type initiator” releases trigger DNAs under a certain condition like warming up to body temperature. The trigger DNAs hybridize to aptamer DNAs on liposomes, and the hybridization causes liposomes collapse. The collapsed liposomes release drugs and new trigger stored inside. These processes are the “chain-reactive burst”, and achieve sequential and exponential release of drugs. <br>
 +
To realize the egg-type initiator, trigger DNAs and chelate compounds are encapsulated in temperature-sensitive liposomes, and the liposomes are encapsulated in alginate hydrogel. Increasing temperature disrupts the liposomes, and then chelate compounds inside the liposomes melt the gels. Collapse of liposomes is realized by rapid deformation of liposomes mediated by hybridization between trigger and aptamer DNAs. <br>
 +
Our system will propose a new place and quantity controlling system of drug release by liposome-based drug delivery systems.<br>
 +
 
 
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</div>

Revision as of 00:01, 12 September 2013

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           <h3>Abstract</h3>

<!--日本語隠すここから--> <!--

          <p>私達のプロジェクトはトリガー入力による連鎖的分子放出システムの構築を目指している。 既存のDDSでは薬剤放出のタイミングの制御が困難な事、一度の入力に対して一定の出力しか得られない事が課題である。</br>

この問題を克服する為、私達はある条件でトリガーDNAを放出するシステムと、その放出を起点に連鎖的にリポソームが割れる出力増幅システムの2つを構築する。</br> 1つ目のシステムでは、トリガーを内在したアルギン酸ゲル膜を作り、温度の上昇に伴い自発的なゲルの融解とトリガーの放出が行われる。2つ目のシステムは、リポソーム表面にトリガーをハイブリし物理的な負荷をかけ、リポソームを破壊する。破壊されるリポソームは新たなトリガーを含んでおり、放出されたトリガーは連鎖的に周囲のリポソームを破壊する。</br> 本システムの実現により入力のタイミングが制御可能かつ一度の入力で大量の薬剤を出力できるDDSの創出等への応用が期待できる。</br>

--> Our project aims to construct a chain-reactive molecule-releasing system in a spontaneous manner. Delivering drugs at appropriate places and controlling the quantity of released drug is very important to develop effective DDS. Our system makes these possible.<br> The system consists of two sub-systems: “egg-type initiator” and “chain-reactive burst”. <br> The “egg-type initiator” releases trigger DNAs under a certain condition like warming up to body temperature. The trigger DNAs hybridize to aptamer DNAs on liposomes, and the hybridization causes liposomes collapse. The collapsed liposomes release drugs and new trigger stored inside. These processes are the “chain-reactive burst”, and achieve sequential and exponential release of drugs. <br> To realize the egg-type initiator, trigger DNAs and chelate compounds are encapsulated in temperature-sensitive liposomes, and the liposomes are encapsulated in alginate hydrogel. Increasing temperature disrupts the liposomes, and then chelate compounds inside the liposomes melt the gels. Collapse of liposomes is realized by rapid deformation of liposomes mediated by hybridization between trigger and aptamer DNAs. <br> Our system will propose a new place and quantity controlling system of drug release by liposome-based drug delivery systems.<br>

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