Difference between revisions of "Biomod/2013/OSU/protocol/folding reaction"

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<h1>Folding Reaction</h1>
 
<h1>Folding Reaction</h1>
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<tr>
 
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<td>p01</td>
 
<td>p01</td>
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<td>p085</td>
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<td>A1</td>
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<td>H12</td>
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<td>CoreP085</td>
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<td>96</td>
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<td>1.04</td>
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<td>core 1</td>
 
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<tr>
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<td>p02</td>
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<td>p085</td>
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<td>A1</td>
<td></td>
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<td>H12</td>
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<td>CoreP086</td>
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<td>41</td>
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<td>2.44</td>
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<td>core 2</td>
 
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<tr>
 
<tr>
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<td>p03</td>
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<td>p086</td>
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<td>E1</td>
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<td>F1</td>
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<td>Neighbors</td>
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<td>13</td>
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<td>7.69</td>
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<td>Neighbors</td>
 
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<tr>
 
<tr>
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<td>p04</td>
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<td>p086</td>
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<td>G1</td>
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<td>H1</td>
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<td>Polymers</td>
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<td>13</td>
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<td>7.69</td>
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<td>Replaced by polymers</td>
 
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Revision as of 20:19, 6 October 2013


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<div id="protocol-content" style="width:65%;margin-left:auto;margin-right:auto;"> <h1>Folding Reaction</h1> <p>A folding reaction is a mixture containing scaffold DNA, staple DNA, water, a buffer mastermix, and magnesium ions. The folding involves subjecting the sample mixture to thermal denaturation followed by renaturing. We will usually have the folding reactions occur in PCR strip tubes of either 50-100 μL aliquots.</p>

<h2>Pre-stocks</h2> <p>A folding reaction is a mixture containing scaffold DNA, staple DNA, water, a buffer mastermix, and magnesium ions. The folding involves subjecting the sample mixture to thermal denaturation followed by renaturing. We will usually have the folding reactions occur in PCR strip tubes of either 50-100 μL aliquots.</p>

<table> <tr> <td><b>Index</b></td> <td><b>Plate</b></td> <td><b>From Well</b></td> <td><b>To Well</b></td> <td><b>Name</b></td> <td><b>No. of Oligos</b></td> <td><b>Conc. (um)</b></td> <td><b>Element</b></td> </tr> <tr> <td>p01</td> <td>p085</td> <td>A1</td> <td>H12</td> <td>CoreP085</td> <td>96</td> <td>1.04</td> <td>core 1</td> </tr> <tr> <td>p02</td> <td>p085</td> <td>A1</td> <td>H12</td> <td>CoreP086</td> <td>41</td> <td>2.44</td> <td>core 2</td> </tr> <tr> <td>p03</td> <td>p086</td> <td>E1</td> <td>F1</td> <td>Neighbors</td> <td>13</td> <td>7.69</td> <td>Neighbors</td> </tr> <tr> <td>p04</td> <td>p086</td> <td>G1</td> <td>H1</td> <td>Polymers</td> <td>13</td> <td>7.69</td> <td>Replaced by polymers</td> </tr> </table>

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