Biomod/2013/OSU/protocol/drugloading

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<a href="http://openwetware.org/wiki/Biomod/2013"> << Back to Team Pages</a>

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                       <a href="http://openwetware.org/wiki/Biomod/2013/OSU/protocol/folding_reaction">
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<a href="http://openwetware.org/wiki/Biomod/2013/OSU/protocol/gel_prep">

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<a href="http://openwetware.org/wiki/Biomod/2013/OSU/protocol/negative_staining">

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<a href="http://openwetware.org/wiki/Biomod/2013/OSU/protocol/TIRF">

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<a href="http://openwetware.org/wiki/Biomod/2013/OSU/protocol/MTT">

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Loading Efficiency

  1. Setup a 2 mM Daunorubicin Solution (Given Concentration of Solution)
  2. Add Origami shapes at a concentration of 5.0 nM
  3. Incubate for 40 hours
  4. Centrifuge at 20.0 xG for 50 minutes @ 20 deg C
  5. Isolate Orange-red precipitate from solution - PEG (Keep solution—gives concentration of daunorubicin not absorbed by origami/used in loading efficiency calculation)
  6. Read Absorption at 488 nm with a microplate reader (FRET Setting)


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