Difference between revisions of "Biomod/2013/Harvard/results"

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(Linker Variations)
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All linker variations showed a decrease in luminescence level, but they all exhibited significant decrease in activity when exposed to high concentration of calcium. Unfortunately, the protein switch does not respond to increased levels of M13,
 
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Revision as of 16:12, 20 October 2013

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Results

Input

Input results

Output

Cysteine Mutations

Mutation Sites.png

Linker Variations

Another possibility we explored was varying the linker length and rigidity. The original GLucCam structure has amino acid strands GGGGT and GGGS as the first and second linker sequence. We explored the following linker variations:

Short-rigid: Short-flexible:

Linker Results.png

All linker variations showed a decrease in luminescence level, but they all exhibited significant decrease in activity when exposed to high concentration of calcium. Unfortunately, the protein switch does not respond to increased levels of M13,