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| <html>
| | [[Image:BITSMOD LOGO.png]] |
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| <head>
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| <link href='http://fonts.googleapis.com/css?family=Amaranth' rel='stylesheet' type='text/css'>
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| <link href='http://fonts.googleapis.com/css?family=Droid+Serif:400,400italic,700,700italic' rel='stylesheet' type='text/css'>
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| </head>
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| <style>
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| /*-----------------------------------------------------------------------------------*/
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| /* GENERAL
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| /*-----------------------------------------------------------------------------------*/
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| /*use #f9f1e6 for #f0f0f0 and */
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| body {
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| background-color: #f9f1e6 ;
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| font-family:Lucida, sans-serif;
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| font-size: 13px;
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| color: #000000;
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| overflow-y: scroll;
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| }
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| .container
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| {
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| background-color: #f9f1e6;
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| margin-top:0px
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| }
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| .OWWNBcpCurrentDateFilled { display: none; }
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| h1, h2, h3, h4, h5, h6 {
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| font-family: 'Amaranth', sans-serif;
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| color: #4a4a4a;
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| font-weight: normal;
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| padding-top: 5px;
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| margin: 0 0 10px 0;
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| }
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| h1 {
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| font-size: 22px;
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| line-height: 36px;
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| }
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| h2 {
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| font-size: 20px;
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| line-height: 24px;
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| }
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| h3 {
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| font-size: 18px;
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| line-height: 22px;
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| }
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| h4 {
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| font-size: 16px;
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| line-height: 20px;
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| }
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| h5 {
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| font-size: 14px;
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| line-height: 20px;
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| }
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| h6 {
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| font-size: 12px;
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| line-height: 18px;
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| }
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| p {
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| line-height: 20px;
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| margin-bottom: 20px;
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| }
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| a {
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| color: #688dad;
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| text-decoration: none;
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| }
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| a:hover {
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| color: #ab4d63;
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| }
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| a.more {
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| font-family: 'Droid Serif', sans serif;
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| font-style: italic;
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| font-size: 13px;
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|
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| }
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| a.button,
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| .forms fieldset .btn-submit,
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| #filtering-nav li a,
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| ul.tabs li a, h2.trigger
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| {
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| text-align: center;
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| background: #FFF url(style/images/button.png) repeat-x left top;
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| padding: 0 20px 0 20px;
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| height: 26px;
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| line-height: 26px;
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| color: #4a4a4a;
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| font-size: 13px;
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| border: 1px solid #dbdbdb;
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| display: inline-block;
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| font-family: 'Amaranth', sans-serif;
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| cursor: pointer;
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| margin-bottom: 20px;
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| -webkit-transition:all 200ms ease-in;
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| -moz-transition:all 200ms ease-in;
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| }
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| a.button:hover,
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| #filtering-nav li.active a,
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| #filtering-nav li a:hover,
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| #filtering-nav li a.active,
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| ul.tabs li a:hover,
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| ul.tabs li.active a,
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| h2.trigger.active, h2.trigger:hover{
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| color: #ab4d63;
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| }
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| a.button.blue {
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| background-color: #718ca4;
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| border: 1px solid #516577;
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| color: #FFF;
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| }
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| a.button.brown {
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| background-color: #a49571;
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| border: 1px solid #877a5c;
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| color: #FFF;
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| }
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| a.button.green {
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| background-color: #71a487;
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| border: 1px solid #578d6e;
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| color: #FFF;
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| }
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| a.button.purple {
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| background-color: #9471a4;
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| border: 1px solid #7b558c;
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| color: #FFF;
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| }
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| a.button.red {
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| background-color: #cb4b4b;
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| border: 1px solid #ba3d3d;
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| color: #FFF;
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| }
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| a.button.blue:hover,
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| a.button.brown:hover,
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| a.button.green:hover,
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| a.button.purple:hover,
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| a.button.red:hover {
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| background-color: #797979;
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| border: 1px solid #5c5c5c;
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| color: #FFF;
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| }
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| .center {
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| text-align: center;
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| display: block;
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| margin: 0 auto;
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| }
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| h3 img {
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| vertical-align: middle;
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| padding-right: 10px;
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| display: inline;
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| }
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| .line {
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| background-color:#d1994a repeat-x;
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| line-height: 2px;
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| padding: 20px 0 0 0;
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| line-break: 0px;
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| }
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| .intro {
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| font-family: 'Droid Serif', serif;
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| font-style: italic;
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| font-size: 17px;
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| color: #000000;
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| line-height: 24px;
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| margin-bottom: 20px;
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| }
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| blockquote p {
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| font-family: 'Droid Serif', serif;
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| font-style: italic;
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| font-size: 14px;
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| color: #000000;
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| line-height: 24px;
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| margin-bottom: 20px;
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| padding-left: 30px;
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| background-color: #d1994a no-repeat left 7px;
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| }
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| ul {
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| line-height: 20px;
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| margin-bottom: 20px;
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| }
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| ul li {
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| background:#f9f1e6 left 8px no-repeat;
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| padding-left: 15px;
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| }
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| ol {
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| list-style: decimal;
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| list-style-position: inside;
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| line-height: 20px;
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| }
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| ol li {
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| padding: 0;
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| margin: 0;
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| }
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| #wrapper
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| {
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| width: 900px;
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| background-color: #f9f1e6;
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| margin-left: auto;
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| margin-right: auto
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| }
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| #content
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| {
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| margin-left: 0px;
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| margin-top: 0px;
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| padding-top: 0px;
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| align: center;
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| /*padding: 12px 12px 12px 12px;
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| width: 30%;
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| background-color: #ffffff; border: 0; */
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|
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| }
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| #sidebar {
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| width: 258px;
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| background:#553816 repeat 100% 0;
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| border-left: 1px solid rgba(0, 0, 0, 0.50);
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| border-right: 1px solid rgba(0, 0, 0, 0.50);
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| float: left;
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| position: relative;
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| margin-top: -1px;
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| z-index: 100;
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| }
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| #logo {
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| text-align: left;
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| padding: 40px 32px 0 32px;
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| }
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| .group:after {
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| content: "";
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| display: table;
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| clear: both;
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| }
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| #footer {
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| background:#f9f1e6
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| overflow: hidden;
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| padding: 20px 0 0 0;
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| margin-top: 40px;
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| }
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| @media screen {
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| body { background: #f9f1e6 0 0 no-repeat; /* changed default background */ }
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| }
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| /*-----------------------------------------------------------------------------------*/
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| /* MENU
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| /*-----------------------------------------------------------------------------------*/
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| #menu {
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| float: left;
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| text-align: left;
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| font-family: 'Amaranth', sans-serif;
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| font-size: 20px;
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| margin-top: 40px;
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| margin-bottom: 50px;
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| position: relative;
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| z-index: 100;
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| padding: 0 0 0 24px;
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| text-shadow: 0px 1px 0px rgba(0, 0, 0, 0.5);
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| }
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| .menu-v ul{
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| margin: 0;
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| padding: 0;
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| list-style-type: none;
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| width: 200px;
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| background:#553816 no-repeat center bottom;
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| }
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|
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| .menu-v ul li{
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| position: relative;
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| padding-right: 5px;
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| background:#553816 no-repeat center top;
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| padding: 0;
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| }
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| .menu-v ul li a{
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| display: block;
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| color:#f3f3f3;
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| text-decoration: none;
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| padding: 12px 0 10px 20px;
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| padding-right: 17px;
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| background: transparent;
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| color:#000000 ;
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| text-decoration: none;
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| border: none;
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| }
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| .menu-v ul li a.active, .menu-v ul li a.selected, .menu-v ul li a:hover{
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| text-decoration: none;
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| background: transparent url(style/images/menu-hover.png) repeat-y left top;
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| color: #ffffff;
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| }
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| .menu-v ul li ul{
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| position: absolute;
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| width: 130px;
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| top: 0px;
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| font-weight: normal;
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| visibility: hidden;
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| text-align: left;
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| padding: 0 15px 0 15px;
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| margin-left: 0;
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| background: #553816 repeat top center;
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| -webkit-box-shadow: inset 0px 0px 0px 1px rgba(255, 255, 255, 0.10);
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| -moz-box-shadow: inset 0px 0px 0px 1px rgba(255, 255, 255, 0.10);
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| box-shadow: inset 0px 0px 0px 1px rgba(255, 255, 255, 0.10);
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| border: 1px solid rgba(0, 0, 0, 0.30);
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| font-size: 14px;
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| line-height: 20px;
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| }
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| .menu-v ul li ul li{
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| background:#553816 repeat-x left top;
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| }
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| .menu-v ul li ul li:first-child {
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| background: none;
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| padding-bottom: 0;
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| }
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| .menu-v ul li ul li a{
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| padding: 12px 0;
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| }
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| .menu-v ul li ul li a.active, .menu-v ul li ul li a.selected, .menu-v ul li ul li a:hover{
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| background: none;
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| }
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| .menu-v ul li ul {
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|
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| }
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| * html .menu-v ul li { float: left; height: 1%; }
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| * html .menu-v ul li a { height: 1%; }
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| </style>
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| </html>
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| <!-- Begin Sidebar -->
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|
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| <div id="sidebar">
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| <div id="logo"><a href="http://openwetware.org/wiki/Biomod/2013/BITS">[[Image:sidelogo.png]]</a>
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| <li><font size="3" color="#ffffff" float="left">BIOMOD 2013</font></li>
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| </div>
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| <!-- Begin Menu -->
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| <div id="menu" >
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|
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| <ul>
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| <li><a href="http://openwetware.org/wiki/Biomod/2013/BITS" >Home</a></li></u1>
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| <li><a href="http://openwetware.org/wiki/Biomod/2013/BITS/background" >Background</a></li>
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| <li><a href="http://openwetware.org/wiki/Biomod/2013/BITS/project">Project</a>
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| <ul>
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| <li><a href="http://openwetware.org/wiki/Biomod/2013/BITS/project1">Project</a></li>
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| <li><a href="http://openwetware.org/wiki/Biomod/2013/BITS/design">Design</a></li>
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|
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| <li><a href="http://openwetware.org/wiki/Biomod/2013/BITS/protocol">Protocols</a></li>
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| </ul></li>
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| <li><a href="http://openwetware.org/wiki/Biomod/2013/BITS/workdone">Work Done</a>
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| <ul>
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| <li><a href="http://openwetware.org/wiki/Biomod/2013/BITS/simulation">Simulation</a></li>
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| <li><a href="http://openwetware.org/wiki/Biomod/2013/BITS/results">Results</a></li> </ul></li>
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| <li><a href="http://openwetware.org/wiki/Biomod/2013/BITS/notebook">Lab Notes</a></li>
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|
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| <li><a href="http://openwetware.org/wiki/Biomod/2013/BITS/team">Team</a></li>
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|
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| </ul>
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| </div>
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|
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| <!-- End Menu -->
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|
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| </div>
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| <!-- End Sidebar -->
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| <!-- Begin Content -->
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|
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| <div id="content">
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| <h1 class="title">Biomimetics at nanoscale: DNA origami to deliver IRES aptamers using HCV as a template</h1>
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| <iframe width="560" height="315" align:center src="#" frameborder="0" allowfullscreen></iframe>
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|
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| <h2>Abstract</h2>
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| <div class="intro">With its worldwide distribution, Hepatitis C Virus (HCV) infects about 3-4 million people every year of which the chronically infected ones are at a risk of developing liver cirrhosis, hepatic failure or hepatocellular carcinoma. The limited availability and poor tolerance towards antiviral therapy and interferon with ribavirin (nucleoside inhibitor) demands a different approach towards targeting the HCV infection. Research indicates that the HCV genome translation is under the control of the Internal Ribosomal Entry Site (IRES) which mediates cap-independent internal initiation of HCV polyprotein translation thereby making it a quintessential target to halt and thereby curb the infection. Nucleic acid ligands generated using SELEX (Systematic Evolution of Ligands by EXponential enrichment) called aptamers, are the recent class of molecules showing immense potential to be crafted for binding to multiple different targets. First proposed by Seeman and started by Rothemund, DNA origami is based on the principle that a long ssDNA scaffold can be molded into any desired shape using staples. With this method of tailoring DNA nanostructures, the self assembling property of nucleic acids would be exploited for the delivery of IRES specific aptamers to the HCV infected cells. We designed a cylindrical nanostructure mimicking HCV by attaching E1-E2 conjugate peptide sequences, facilitating the entry via endosomal pathway; and by using pH sensitive detachable lids to release the IRES aptamers packed inside the nanostructure to the infected cells. The aptamer based silencing of viral translation will in turn prevent the assembly of new viroids and serve as a mechanism to control the HCV infection.</div>
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| </div>
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| <!--End Content -->
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