Difference between revisions of "Biomod/2012/UCSD/tRiton Nano Architects"

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'''''This wiki page is under construction'''''
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{{Biomod/2012/UCSD/UCSDtemplate}}
  
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<center>
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=Abstract: Fluorescent DNA Aptamer for lysozyme detection =
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</center>
  
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Combining previous work on structure-switching signaling aptamers and fluorescent signaling we design a DNA based aptamer modified with fluorophores and quenchers to detect and signal the presence of lysozyme. This device is then compared against the same attached with a nanoparticle. We verify the device’s behavior using polyacrylamide gel electrophoresis, atomic force microscopy, and fluorescent spectroscopy. Our device demonstrates that DNA nano-structures can be designed to meet detection of specific and trace quantities of proteins and this can have important applications such as low cost sensors for detecting deadly viruses via viral capsid proteins.
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<center>
  
*Team Name: tRiton Nano Architects
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=Video=
*Institution Name: University of California, San Diego
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</center>
*Geographic Location: San Diego, California, United States
 
  
  
'''''Member page''''': http://openwetware.org/wiki/Biomod/2012/UCSD/tRiton_Nano_Architects/members
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<html><center>
  
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<iframe width="712" height="400" src="http://www.youtube.com/embed/jPkbv64CIL8?rel=0&vq=hd720" frameborder="0" allowfullscreen></iframe>
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</center>
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</html>
  
=Discussion=
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<div>
===Progress Log===
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<center>
*Gathered reference material
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[[Biomod/2012 | Back to the Biomod 2012 webpage]]
*Brainstormed potential ideas
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</center>
 
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</div>
===Things to do===
 
*Refine ideas
 
*Summer Project
 
*Build wiki page
 
*Youtube video
 
 
 
=Abstract=
 

Latest revision as of 21:37, 27 October 2012

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<html><center><a href='http://openwetware.org/wiki/Biomod/2012/UCSD/tRiton_Nano_Architects'><img src=http://openwetware.org/images/a/a0/Trna_logo_with_title5.png width=1118px></a></center></html> <html><center><a href='http://openwetware.org/wiki/Biomod/2012/UCSD/tRiton_Nano_Architects'><img src=http://openwetware.org/images/e/e5/Menuspacer2.png width=8px></a></center></html>

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  <li class='active has-sub '><a href='http://openwetware.org/wiki/Biomod/2012/UCSD/tRiton_Nano_Architects'><span>Home</span></a>
     <ul>
        <li><a href='http://openwetware.org/wiki/Biomod/2012/UCSD/tRiton_Nano_Architects#Abstract:_Fluorescent_DNA_Aptamer_for_lysozyme_detection'><span>Abstract</span></a></li>
        <li><a href='http://openwetware.org/wiki/Biomod/2012/UCSD/tRiton_Nano_Architects#Video'><span>Video</span></a></li>
     </ul>
  </li>
  <li class='has-sub '><a href='http://openwetware.org/wiki/Biomod/2012/UCSD/tRiton_Nano_Architects/Project_introduction'><span>Project Introduction</span></a>
     <ul>
        <li><a href='http://openwetware.org/wiki/Biomod/2012/UCSD/tRiton_Nano_Architects/Project_introduction#Concepts'><span>Concepts</span></a></li>
     </ul>
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  <li class='has-sub '><a href='http://openwetware.org/wiki/Biomod/2012/UCSD/tRiton_Nano_Architects/Experiment'><span>Experiment</span></a>
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        <li><a href='http://openwetware.org/wiki/Biomod/2012/UCSD/tRiton_Nano_Architects/Experiment#Methods'><span>Methods</span></a></li>
     </ul>
  </li>
  <li class='has-sub '><a href='http://openwetware.org/wiki/Biomod/2012/UCSD/tRiton_Nano_Architects/Resources'><span>Supplementary Resources</span></a>
     <ul>
        <li><a href='http://openwetware.org/wiki/Biomod/2012/UCSD/tRiton_Nano_Architects/Brainstorm#DNA_architecture_log'><span>DNA Architecture Log</span></a></li>
        <li><a href='http://openwetware.org/wiki/Biomod/2012/UCSD/tRiton_Nano_Architects/Brainstorm#Project_Ideas'><span>Project Ideas</span></a></li>
        <li><a href='http://openwetware.org/wiki/Biomod/2012/UCSD/tRiton_Nano_Architects/Literature'><span>Literature</span></a></li>
     </ul>
  </li>
  <li><a href='http://openwetware.org/wiki/Biomod/2012/UCSD/tRiton_Nano_Architects/Team'><span>Team</span></a></li>

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Abstract: Fluorescent DNA Aptamer for lysozyme detection

Combining previous work on structure-switching signaling aptamers and fluorescent signaling we design a DNA based aptamer modified with fluorophores and quenchers to detect and signal the presence of lysozyme. This device is then compared against the same attached with a nanoparticle. We verify the device’s behavior using polyacrylamide gel electrophoresis, atomic force microscopy, and fluorescent spectroscopy. Our device demonstrates that DNA nano-structures can be designed to meet detection of specific and trace quantities of proteins and this can have important applications such as low cost sensors for detecting deadly viruses via viral capsid proteins.

Video


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