Difference between revisions of "Biomod/2012/Tohoku/Team Sendai"

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{{:Biomod/2012/Tohoku/Team_Sendai/header}}
 
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<!—二段組み本文 -->
 
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<h1 style="margin-top:-50px;"><!--Team Sendai--><img src="http://openwetware.org/images/c/c2/Title-kai.png" ></h1>
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<h1 align="center";><!--Team Sendai--><img src="http://openwetware.org/images/c/c2/Title-kai.png" ></h1>
<p>Tohoku University</p>
 
 
</div>
 
</div>
  
ロゴはもうちょっと考えるべきかも
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<h1>Abstract</h1>
 
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<p>
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All the creatures on Earth are made of cells. The exterior and the interior of the cell are compartmentalized by biomembranes.</br>
 +
A nanodevice that is able to actively transport only the specific oligonucleotide through the biomembrane has a great potential to deliver siRNA into the cell or to extract mRNA expressed in the cell.</br>
 +
Here, we decided to create a novel device with such a function. </br>
 +
We have designed <strong>a cylindrical injector/extractor device made of DNA origami</strong> (we named this device: <strong>CELL-GATE</strong>). Inside the cylinder, a cascade of single stranded DNAs is planted. Once the outer-most ssDNA binds to a target oligonucleotide, the target is passed to the inner-ones one by one because of the higher bonding energy assigned to the inner ones.</br>
 +
We also investigate how the cylinder penetrates the biomembrane by using liposome as a model membrane.</br>
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</p>
  
<div id="Container">
 
<!—二段組み本文 -->
 
 
<h1 style="width:900px;clear:left;margin:15px;">
 
Abstract</h1>
 
<p style="width:900px;clear:left;margin:15px;">
 
&nbsp;All the creatures on Earth are made of cells. The exterior and the interior of the cell are compartmentalized by biomembranes.
 
A nanodevice that is able to actively transport only the specific oligonucleotide through the biomembrane has a great potential to deliver siRNA into the cell or to extract mRNA expressed in the cell.<br>
 
Here, we decided to create a novel device with such a function. <br>
 
We have designed a cylindrical injector/extractor device made of DNA origami. Inside the cylinder, a cascade of single stranded DNAs is planted. Once the outer-most ssDNA binds to a target oligonucleotide, the target is passed to the inner-ones one by one because of the higher bonding energy assigned to the inner ones.<br>
 
We also investigate how the cylinder penetrates the biomembrane by using liposome as a model membrane.<br>
 
<br>
 
</p>
 
  
  
 
<div id="pop">
 
<div id="pop">
 
<h2><!--Abstract-->Project Image</h2>
 
<h2><!--Abstract-->Project Image</h2>
<img src="http://openwetware.org/images/c/c5/Projectcellgate.png" alt="D-Heart" width="400px"><br>
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<p>
<strong>Porter</strong>: Single stranded DNA. Once the outer-most ssDNA binds to a target, the target is passed to the inner-ones one by one.</br>
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<strong>Our PROJECT CELL-GATE is divided into three sub-projects:</strong><br>
<strong>Gate</strong>: Cylindrical structure connecting the inside and outside like gates. A cascade of the "Selector" is planted in this. We can use this as an injector or extractor.</br>
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<a href="http://openwetware.org/wiki/Biomod/2012/TeamSendai/Idea#PORTER">PORTER</a>: Composed of single stranded DNA (ssDNA) sequences. Each ssDNA is called  Porter. Porters are designed to transfer target DNA strands into (or out from) the membrane.</br>
<strong>Membrane</strong>: The exterior and the interior of the cell are compartmentalized by biomembranes. We use liposome as a model membrane.
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<a href="http://openwetware.org/wiki/Biomod/2012/TeamSendai/Idea#GATE">GATE</a>: Cylindrical DNA nanostructure connecting the inside and outside of membrane like a channel. A cascade of the Porters is planted inside this cylinder. We can use this Gate as an injector or an extractor.</br>
 +
<a href="http://openwetware.org/wiki/Biomod/2012/TeamSendai/Idea#MEMBRANE">MEMBRANE</a>: The exterior and the interior of the cell are compartmentalized by biomembranes. We use liposome as a model membrane.
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</p>
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<img src="http://openwetware.org/images/c/c5/Projectcellgate.png" alt="D-Heart" width="440px" usemap="#D-Heart">
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<map name="D-Heart">
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<area shape="rect" coords="310,8,390,45" href="http://openwetware.org/wiki/Biomod/2012/TeamSendai/Idea#PORTER" alt="Porter">
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<area shape="rect" coords="310,95,370,132" href="http://openwetware.org/wiki/Biomod/2012/TeamSendai/Idea#GATE" alt="Gate">
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<area shape="rect" coords="33,213,165,245" href="http://openwetware.org/wiki/Biomod/2012/TeamSendai/Idea#MEMBRANE" alt="Membarane">
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</map>
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Please CLICK ITEMS, GATE, PORTER, AND MEMBRANE in the picture!
 
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<h2>Movie</h2>
 
<h2>Movie</h2>
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<p>Coming soon</p>
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<iframe width="420" height="315" src="http://www.youtube.com/embed/8OB8ytltxwY" frameborder="0" allowfullscreen></iframe>
<br><br>
 
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<youtube align="center" width="400px" height="291px">Xpl3sRCQ</youtube>
 
 
 
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<td style="color:white;">Coming soon...</td>
 
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</p>
 
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<br clear = "left">
  
  
<a href=" http://openwetware.org/wiki/Biomod/2012/Team_Sendai_Old">Test page</a>
 
  
 
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Latest revision as of 19:43, 27 October 2012

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<body> <div id="Container"> <!-- Menu --> <ul id="menu"> <li><a href="http://openwetware.org/wiki/Biomod/2012/Tohoku/Team_Sendai ">Home</a></li> <li><a href=" http://openwetware.org/wiki/Biomod/2012/TeamSendai/Idea ">Project</a></li> <li><a href=" http://openwetware.org/wiki/Biomod/2012/TeamSendai/Design">Design</a> </li> <li><a href=" http://openwetware.org/wiki/Biomod/2012/TeamSendai/Simulation">Simulation</a> </li> <li> <a href=" http://openwetware.org/wiki/Biomod/2012/TeamSendai/Experiment ">Experiment</a> </li> <li> <a href=" http://openwetware.org/wiki/Biomod/2012/TeamSendai/Future Application">Future Application</a> </li> <li> <a href=" http://openwetware.org/wiki/Biomod/2012/TeamSendai/Diary">Diary</a> </li> <li> <a href=" http://openwetware.org/wiki/Biomod/2012/TeamSendai/Team ">Team</a> </li> <li> <a href=" http://openwetware.org/wiki/Biomod/2012/Tohoku/Team Sendai/header"></a> </li>

</ul>

<div id="ttop"> <a href="#top" class="page_top" onfocus="this.blur();" onclick="scrollTo(0,0); return false;" title="Top"></a></div>

</body> </div> </html> <html> <div id="Container"> <!—二段組み本文 --> <div id="Header"> <h1 align="center";><!--Team Sendai--><img src="http://openwetware.org/images/c/c2/Title-kai.png" ></h1> </div>

<h1>Abstract</h1> <p> All the creatures on Earth are made of cells. The exterior and the interior of the cell are compartmentalized by biomembranes.</br> A nanodevice that is able to actively transport only the specific oligonucleotide through the biomembrane has a great potential to deliver siRNA into the cell or to extract mRNA expressed in the cell.</br> Here, we decided to create a novel device with such a function. </br> We have designed <strong>a cylindrical injector/extractor device made of DNA origami</strong> (we named this device: <strong>CELL-GATE</strong>). Inside the cylinder, a cascade of single stranded DNAs is planted. Once the outer-most ssDNA binds to a target oligonucleotide, the target is passed to the inner-ones one by one because of the higher bonding energy assigned to the inner ones.</br> We also investigate how the cylinder penetrates the biomembrane by using liposome as a model membrane.</br> </p>


<div id="pop"> <h2><!--Abstract-->Project Image</h2> <p> <strong>Our PROJECT CELL-GATE is divided into three sub-projects:</strong><br> <a href="http://openwetware.org/wiki/Biomod/2012/TeamSendai/Idea#PORTER">PORTER</a>: Composed of single stranded DNA (ssDNA) sequences. Each ssDNA is called Porter. Porters are designed to transfer target DNA strands into (or out from) the membrane.</br> <a href="http://openwetware.org/wiki/Biomod/2012/TeamSendai/Idea#GATE">GATE</a>: Cylindrical DNA nanostructure connecting the inside and outside of membrane like a channel. A cascade of the Porters is planted inside this cylinder. We can use this Gate as an injector or an extractor.</br> <a href="http://openwetware.org/wiki/Biomod/2012/TeamSendai/Idea#MEMBRANE">MEMBRANE</a>: The exterior and the interior of the cell are compartmentalized by biomembranes. We use liposome as a model membrane. </p>

<img src="http://openwetware.org/images/c/c5/Projectcellgate.png" alt="D-Heart" width="440px" usemap="#D-Heart"> <map name="D-Heart"> <area shape="rect" coords="310,8,390,45" href="http://openwetware.org/wiki/Biomod/2012/TeamSendai/Idea#PORTER" alt="Porter"> <area shape="rect" coords="310,95,370,132" href="http://openwetware.org/wiki/Biomod/2012/TeamSendai/Idea#GATE" alt="Gate"> <area shape="rect" coords="33,213,165,245" href="http://openwetware.org/wiki/Biomod/2012/TeamSendai/Idea#MEMBRANE" alt="Membarane"> </map> Please CLICK ITEMS, GATE, PORTER, AND MEMBRANE in the picture! </div>


<div id="pop"> <h2>Movie</h2> <p> </br> <iframe width="420" height="315" src="http://www.youtube.com/embed/8OB8ytltxwY" frameborder="0" allowfullscreen></iframe> </p> </div> <br clear = "left">


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