Difference between revisions of "Biomod/2012/Titech/Nano-Jugglers/Results/EDAC conjugation"

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|'''Not observed'''
 
|'''Not observed'''
 
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[[Image:EDAC conjugation.jpg|300px]]
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[[Image:EDAC conjugation.jpg|400px]]
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:※We use polystyrene beads whose 1/4 was covered with Gold and 1/2 was covered with chromium.<br><br>
 
:※We use polystyrene beads whose 1/4 was covered with Gold and 1/2 was covered with chromium.<br><br>
  

Latest revision as of 23:06, 3 October 2012


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} </style> </head> <BODY> <div id="biomodlink"> <<a href="http://openwetware.org/wiki/Biomod">BIOMOD</a>|<a href="http://openwetware.org/wiki/Biomod/2012">2012</a>|Titech Nano-Jugglers </div> <div id="header"> <div id="navigation"> <div id="menu"> <ul> <li><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers"><br>Home<br><br></a></li> <li><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers/Team/Students"><br>Team<br><br></a></li> <li><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers/Project"><br>Project<br><br></a></li> <li><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers/Results">Results<br>&<br>Methods</a></font></li> <li class="ach"><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers/Achievements"><br>Achievements<br><br></a> <li class="sup"><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers/Protocols"><br>Suppl. Info.<br><br></a></li> <li class="none"><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers/Acknowledgement"><br>Acknowledgements<br><br></a></li> </ul> </div> </div> </div> </BODY> </html>


Amino modified DNA and polystyrene conjugation by EDAC

After the protocol, to confirm that amino modified DNA is bind to polystyrene ,we hybridize the DNA with FAM and observe them under blue light by microscope.(Control experiment)

RESULT

Added Reagent Polystyren beads
DNA which bind with beads
Complementary FAM
Polystyren beads
DNA which bind with beads

Polystyren beads

Complementary FAM
Under Transmitted Light BIOMOD-TNJ-CrAu 1 2.jpg BIOMOD-TNJ-CrAu 2.jpg BIOMOD-TNJ-CrAu 3.jpg
Under Blue Light BIOMOD-TNJ-CrAu 1 2 2s.jpg BIOMOD-TNJ-CrAu 2 2s.jpg BIOMOD-TNJ-CrAu 3 2s.jpg
Fluorescence observed Not observed Not observed

EDAC conjugation.jpg

※We use polystyrene beads whose 1/4 was covered with Gold and 1/2 was covered with chromium.

From this result, we concluded that amino modified DNA is bind to polystyrene

Observation Conditions

ISO6400
Exposure time(Transmitted Light)1/100seconds
Exposure time(Blue Light)2seconds
Magnification 10×40=400