Biomod/2012/Titech/Nano-Jugglers/Project: Difference between revisions
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==2.Background== | ==2.Background== | ||
:: Break through the limits of speed― It is one of the most difficult goal, and we are attracted on it. As well as its own attractive, speeding up is also greatly benefit to our lives, such as mass transport and signal transduction. | :: Break through the limits of speed― It is one of the most difficult goal, and we are attracted on it. As well as its own attractive, speeding up is also greatly benefit to our lives, such as mass transport and signal transduction. | ||
Revision as of 23:08, 3 October 2012
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} </style> </head> <BODY> <div id="biomodlink"> <<a href="http://openwetware.org/wiki/Biomod">BIOMOD</a>|<a href="http://openwetware.org/wiki/Biomod/2012">2012</a>|Titech Nano-Jugglers </div> <div id="header"> <div id="navigation"> <div id="menu"> <ul> <li><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers"><br>Home<br><br></a></li> <li><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers/Team/Students"><br>Team<br><br></a></li> <li><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers/Project"><br>Project<br><br></a></li> <li><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers/Results">Results<br>&<br>Methods</a></font></li> <li class="ach"><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers/Achievements"><br>Achievements<br><br></a> <li class="sup"><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers/Protocols"><br>Suppl. Info.<br><br></a></li> <li class="none"><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers/Acknowledgement"><br>Acknowledgements<br><br></a></li> </ul> </div> </div> </div> </BODY> </html>
Project
1.Goals
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We have set 3 goals high-speed rail-free photo-control |
2.Background
- Break through the limits of speed― It is one of the most difficult goal, and we are attracted on it. As well as its own attractive, speeding up is also greatly benefit to our lives, such as mass transport and signal transduction.
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- In this way, we came to the following conclusion that we should make a completely new molecular robot, 'Biomolecular Rocket'.
3.Idea
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Rocket is one of the fastest vehicle that can be controlled and not only these features, but also rocket have innovative features, 'Rail-Free'. That means, rocket can move around freely in a long distance, while other molecular robots need rails to move, so their movement is limited. |
- Upon building rocket, to product energy for rail-free movement, we looked at the function of the platinum catalyst. Platinum decompose H2O2 and emit H2O and O2 bubbles. The dissolved O2 surrounding a bubble continues to diffuse into the bubble causing it to grow while the buoyancy force and surface adhesion compete against one another. While O2 detached from platinum surface, a momentum change which induces a driving force away from the surface. So, by placing the platinum on the back of the rocket body, platinum will move forward. A new bubble will be generated and released as long as hydrogen peroxide is present, and so the platinum is continuously propelled in the solution.
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To conjugate platinum to the body, we took advantage of DNA hybridization and denaturation. We conjugated numerous nano-scale platinum particles to a micrometer-sized rocket body with the use of DNA. By this way, we can increase the emission of O2bubbles from the rocket.In addition, direction of the rail-free movement of our rocket can be controlled, since we designed the photoresponsive DNA for allowing detachment of the engines from the body upon the UV light irradiation in a region-specific manner. In other words, by using the DNA, not only our rocket will be speeding up, but also we will be able to handle the rocket easily. |
We have created the most simplest rocket that combines these three features.
- Why DNA?
- There are three reasons of using DNA. First of all, we can connect Platinum particles with Rocket body in a region-specific because DNA has complementarity. If we bind DNA single strand to the target point, the target molecular which has the complementary DNA, would hybridize and connect with the target point naturally.
- For the second, it is necessary to detach catalytic engine in a region-specific manner. While there are a variety of ways, using the denaturation of photoresponsive DNA can be done very easily than others.
- Thirdly, there is a merit that we can conjugate each material with use of only one way.If we use several ways to conjugate in response to each material, it is difficult for each conjugation to stay one another because their suitable conditions are different, such as pH and temperature.However, by using DNA, we can conjugate various materials at time due to the DNA’s characteristic, because DNA’s conjugation depends on the base sequences. So, if we design DNA’s base sequences, it enables each conjugation at the time
- This study embodies the concept to utilize and control non-biological reactions by designing biomolecules for achieving novel functions implemented by synthetic molecular systems.
4.Project Overview
0.Body construction
- Biomolecular Rocket is consisted of 10μm beads, platinum or catalase, and DNA. By using DNA, platinum or catalase particles are conjugated to 10μm beads. Beads have been addressed by the deposition of gold and chromium, so DNA was able to conjugate to beads region-specific. In addition, we designed the photoresponsive DNA for allowing detachment of the engines from the Biomolecular rocket’s body upon the UV light irradiation in a region-specific manner.
1,Energy production for rail-free movement of Biomolecular Rocket
- DNA spider moves forward by controlling of Brownian motion and kinesin motor gains energy of ATP hydrolysis. These movements are carried out through the rails. In other words, power and direction of these movements are bound by rails.
- Energy production of Biomolecular Rocket isn’t depended on rails. Our rocket utilizes H2O2 that is included in the solution. Platinum particles and catalase are catalysts that have the ability to decompose hydrogen peroxide into oxygen and water. Since the driving force created by divergence of bubbles, and rocket proceeds by dissociation of oxygen, rail does not require.
2,Speeding up of Biomolecular Rocket
- Platinum hemisphere emit sufficient amount of bubbles to move forward in a dilute H2O2 solution. Emission of the bubbles depends on the surface area of catalyst. If the surface area is expanded, it is obvious that our rocket will be able to emit more bubbles and speeding up. So, we conjugated numerous catalytic engines to a rocket body with the use of DNA in order to expend surface area of catalyst.
- We conjugate between 10 μm polystyrene beads and 0.15 ~ 0.40 μm sized platinum. In this condition, approximately 2000 of platinum particles can be conjugated to the hemisphere. This suggests that comparison the value of platinum surface area, platinum particles enable to get more surface than equally size of platinum hemisphere. Our rocket got of the surface area 3.6 times in the calculation
- That means, we can significantly increase the surface area of the catalytic reaction.
3,Directional control of Biomolecular Rocket
- Direction of the rail-free movement of our rocket can be controlled, since we designed the photoresponsive DNA. Photoresponsive DNA structure is changed by UV light irradiation, then denaturation of double strand DNA will happen. This reaction allows the detachment of the engines from the body upon the UV light irradiation in a region-specific manner. Difference in the amount of bubbles are generate at the position of the body. So, we can change the direction of our rocket by only irradiating UV light.
4-1 BODY
- The body is made of polystyrene beads, Pt beads and DNA. Polystyrene beads are processed to conjugate different kinds of DNA.
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Vapor deposition In order to have a positional specificity, We make metal-deposited beads. Hemispherical part of polystyrene bead is covered with Cr,1/4 part of spere is covered with Au and the other part is polystyrene. |
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Amino modified DNA and polystyrene conjugation |
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SAM(Self-assembled Monolayer) |
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Bond photoresponsive DNA with Au and bond normal DNA with Polystyrene.When we add Pt which has complementary DNA,we can connect JET's BODY with Pt regiospecifically. |
4-2 DRIVING FORCE
Hydrogen peroxide is decomposed into water and oxygen by the catalyst, and platinum and catalase cause the decomposition reaction. This reaction creates a high concentration of oxygen gas on the catalyst surface. The bubble continues to grow until it reaches the detachment radius and is released from the surface. The detachment induces a driving force from the catalyst surface.
4-3 CONTROL
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Photo switching system The formation of DNA which includes azobenzen can be changed by UV light irradiation. When we irradiate UV light on photoresponsive DNA, DNA will be dissociated. By adjusting the position of photoresponsive DNA, we can control the direction of biomolecular rocket’s moving. We bond photoresponsive DNA with Au, and normal DNA with polystyrene and also each complementary DNAs with Pt. |
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When we irradiate UV light on biomolecular rocket, Pt beads are cut off from Au and the power from Au side disappears and finally, the direction of JET’s power would be changed.
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