Biomod/2012/Titech/Nano-Jugglers/Methods/Metal area

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} </style> </head> <BODY> <div id="biomodlink"> <<a href="http://openwetware.org/wiki/Biomod">BIOMOD</a>|<a href="http://openwetware.org/wiki/Biomod/2012">2012</a>|Titech Nano-Jugglers </div> <div id="header"> <div id="navigation"> <div id="menu"> <ul> <li><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers"><br>Home<br><br></a></li> <li><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers/Team/Students"><br>Team<br><br></a></li> <li><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers/Project"><br>Project<br><br></a></li> <li><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers/Results">Results<br>&<br>Methods</a></font></li> <li class="ach"><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers/Achievements"><br>Achievements<br><br></a> <li class="sup"><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers/Protocols"><br>Suppl. Info.<br><br></a></li> <li class="none"><a href="http://openwetware.org/wiki/Biomod/2012/Titech/Nano-Jugglers/Acknowledgement"><br>Acknowledgements<br><br></a></li> </ul> </div> </div> </div> </BODY> </html>

0.3. Selective DNA conjugation to the microbead body of Biomolecular Rocket

0.3.2. Selective conjugation of DNA to metal surface area


<html><body><td align="center" width="150px"><img src="http://openwetware.org/images/0/01/BIOMODTNJSAM3.png" border=0 width=300 height=240></a></td></body></html>

    Selective conjugation of DNA to metal surface area is realized with Self-assembled monolayers (SAM)[1],[2]. SAM of molecules are molecular assemblies formed spontaneously on substrate surfaces by chemical adsorption. This chemical reactions can be organized into large ordered domains and it is also possible to cover the substrate with functional end group that has some modification. In addition, few steps and experimental technique are required. SAMs are created by adsorption of head groups onto a substrate followed by a slow organization of tail groups. First, head groups are absorbed onto substrate over. In this phase, the surface of substrate is patterned with spots by dsorbeate molecules rather than beautiful membranes.

   Over the period of disordering form, the head groups assemble together on the substrate, while the tail groups assemble far from the substrate. Areas of concentrated molecules nucleate and grow until the surface of the substrate is covered in a single monolayer.
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DNA conjugation onto gold
    Thiolated DNA sequence S* compound and gold substrate are one of the most famous research of SAMs[3]. Thiol modified DNA was conjugate to gold particles by this chemical reaction. Initially, substrate gold were cleaned by water or acid-base buffer in order to washout organic impurities. Gold particles were dissolved in 10 mM phosphate buffer pH8, 10 μM thiol modified DNA.

Reference
[1] A. Ulman, Chem Rev 96, 1533 (1996).
[2] G. E. Poirier, Chem Rev 97, 1117 (1997).
[3]Dmitri Y. Petrovykh,; Hiromi Kimura-Suda,; Aric Opdahl,; Lee J. Richter,; Michael J. Tarlov,; Lloyd J. Whitman.Langmuir 22, 2578-2587(2006)
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