Difference between revisions of "Biomod/2012/Titech/Nano-Jugglers/Methods/Design of photoresponsive DNA"

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(3.1. Design of photoresponsive DNA)
(3.1. Design of photoresponsive DNA)
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1.This 11 bases DNA are a complementary sequence of photoresponsive DNA sequence S.
 
1.This 11 bases DNA are a complementary sequence of photoresponsive DNA sequence S.
 
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:&nbsp;&nbsp;&nbsp;&nbsp;And then, we confirmed that photoresponsive DNA could hybridize stably. For verifying hybridization, we use the value of absorbance.<br>
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:&nbsp;&nbsp;&nbsp;&nbsp;And then, we confirmed that photoresponsive DNA could hybridize stably. For verifying hybridization, we used the value of absorbance.<br>
 
:&nbsp;&nbsp;&nbsp;&nbsp;In spectroscopy, a relational expression about absorbance can be expressed as follows.
 
:&nbsp;&nbsp;&nbsp;&nbsp;In spectroscopy, a relational expression about absorbance can be expressed as follows.
 
:[[Image:Math.png|center|200px]]
 
:[[Image:Math.png|center|200px]]

Revision as of 03:21, 26 October 2012


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3.1. Design of photoresponsive DNA

    We incorporated 4 azobenzene units into photoresponsive DNA[1] strand so that we can quickly dissociated DNA duplex. We called it as Photoresponsive DNA sequence S.[5'-AATXACXCCXAGXCC-3' (X=Azobenzene)]
BIOMOD-TNJ-azobenzen5.png

Photoresponsive DNA sequence S

  • 5’ -(HS)-AATxACxCCxAGxCC-3’ (x=azobenzene)

1.We incorporated 4 azobenzene units into 11 bases DNA so that we can quickly dissociated DNA duplex.

BIOMOD-TNJ-azobenzen7.png

Photoresponsive DNA sequence S*

  • 5’-(HS)-GGCTGGGTATT-3’

1.This 11 bases DNA are a complementary sequence of photoresponsive DNA sequence S.

    And then, we confirmed that photoresponsive DNA could hybridize stably. For verifying hybridization, we used the value of absorbance.
    In spectroscopy, a relational expression about absorbance can be expressed as follows.
Math.png
    (Abs: absorbance, ε: the absorption coefficient, c: strength, d: length of a visual leg )
    The value of Abs is proportional to the concentration of corresponding materials. If photoresponsive DNA formed duplex, the concentration of ssDNA decrease, onthe other hand the concentration of dsDNA increase. Reserchin these corresponding values, we could determine whether they formed duplex or not.
Reference
[1]Hiroyuki Asanuma, Xingguo Liang, Takayuki Yoshida, and Makoto Komiyama,;CHEMBIOCHEM 2, 39-44(2001)
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