Difference between revisions of "Biomod/2012/Titech/Nano-Jugglers/Methods/Design of photoresponsive DNA"

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(3-1.Design of photoresponsive DNA)
(3.1. Design of photoresponsive DNA)
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{{Titech/Nano-Jugglers/HEAD}}
 
{{Titech/Nano-Jugglers/HEAD}}
 
==3.1. Design of photoresponsive DNA==
 
==3.1. Design of photoresponsive DNA==
:&nbsp;&nbsp;&nbsp;&nbsp;We incorporated 4 azobenzene units into photoresponsive DNA<sup>[1]</sup> strand so that we can quickly dissociated DNA duplex.{Photo-switchable DNA 5'-AATXACXCCXAGXCC-3' (X=Azobenzene)}
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:&nbsp;&nbsp;&nbsp;&nbsp;We incorporated 4 azobenzene units into photoresponsive DNA<sup>[1]</sup> strand so that we can quickly dissociated DNA duplex. We called it as Photoresponsive DNA sequence S.[5'-AATXACXCCXAGXCC-3' (X=Azobenzene)]
 
:{|
 
:{|
 
|-
 
|-
 
|width="150px"|[[Image:BIOMOD-TNJ-azobenzen5.png|left|150px]]
 
|width="150px"|[[Image:BIOMOD-TNJ-azobenzen5.png|left|150px]]
 
|
 
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photoresponsive DNA<br>
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Photoresponsive DNA sequence S<br>
 
*5’ -(HS)-AATxACxCCxAGxCC-3’ (x=azobenzene)
 
*5’ -(HS)-AATxACxCCxAGxCC-3’ (x=azobenzene)
 
1.We incorporated 4 azobenzene units into 11 bases DNA so that we can quickly dissociated DNA duplex.
 
1.We incorporated 4 azobenzene units into 11 bases DNA so that we can quickly dissociated DNA duplex.
 
|width="150px"|[[Image:BIOMOD-TNJ-azobenzen7.png|left|150px]]
 
|width="150px"|[[Image:BIOMOD-TNJ-azobenzen7.png|left|150px]]
 
|
 
|
cDNA of photoresponsive DNA<br>
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Photoresponsive DNA sequence S<sup>*<sup><br>
 
*5’-(HS)-GGCTGGGTATT-3’
 
*5’-(HS)-GGCTGGGTATT-3’
1.This 11 bases DNA are a complementary sequence of DNAⅰ(photoresponsive DNA).
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1.This 11 bases DNA are a complementary sequence of photoresponsive DNA sequence S.
 
|}
 
|}
:&nbsp;&nbsp;&nbsp;&nbsp;And then, we confirmed that photoresponsive DNA could hybridize stably at consistent temperature. For verifying hybridization, we use the value of absorbance.<br>
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:&nbsp;&nbsp;&nbsp;&nbsp;And then, we confirmed that photoresponsive DNA could hybridize stably. For verifying hybridization, we use the value of absorbance.<br>
 
:&nbsp;&nbsp;&nbsp;&nbsp;In spectroscopy, a relational expression about absorbance can be expressed as follows.
 
:&nbsp;&nbsp;&nbsp;&nbsp;In spectroscopy, a relational expression about absorbance can be expressed as follows.
 
:[[Image:Math.png|center|200px]]
 
:[[Image:Math.png|center|200px]]

Revision as of 22:49, 25 October 2012


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