Difference between revisions of "Biomod/2012/Titech/Nano-Jugglers/Methods"

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(0.3.2. Selective conjugation of DNA to metal surface area)
(0.4. Catalyst attachment with DNA hybridization)
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&nbsp;&nbsp;&nbsp;&nbsp;'''We attached the catalyst to our rocket body with DNA hybridization.''' cDNA had adhered to the corresponding parts of the rocket body, so it enabled to attach catalyst engines in that we designed these DNA were thermodynamically stable when they hybridized.<br><br>
 
&nbsp;&nbsp;&nbsp;&nbsp;'''We attached the catalyst to our rocket body with DNA hybridization.''' cDNA had adhered to the corresponding parts of the rocket body, so it enabled to attach catalyst engines in that we designed these DNA were thermodynamically stable when they hybridized.<br><br>
&nbsp;&nbsp;&nbsp;&nbsp;First, we prepared DNA-conjugated 10 μm sized polystyrene beads which were deposited 1/4 Au and 1/2 Cr.  At the same time we prepared DNA-conjugated 0.15-0.40 μm sized platinum particles. Then, mixed these materials in 3×SSC buffer at 90℃, and cooled to room temperature slowly over time. Namely, annealing DNA by raising temperature at the first time, next make it easy to hybridize each other by downing to room temperature.
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&nbsp;&nbsp;&nbsp;&nbsp;First, we prepared DNA-conjugated 10 μm sized polystyrene beads which were deposited 1/4 Au and 1/2 Cr.  At the same time we prepared DNA-conjugated 0.15-0.40 μm sized platinum particles. Then, mixed these materials in 3×SSC buffer at 90 °C, and cooled to room temperature slowly over time. Namely, annealing DNA by raising temperature at the first time, next make it easy to hybridize each other by downing to room temperature.
 
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::::::::::::>>back to [[Biomod/2012/Titech/Nano-Jugglers/Results#0.4._Catalyst_attachment_with_DNA_hybridization|Results]]
 
::::::::::::>>back to [[Biomod/2012/Titech/Nano-Jugglers/Results#0.4._Catalyst_attachment_with_DNA_hybridization|Results]]
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