Biomod/2011/TUM/TNT/Results: Difference between revisions

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The purification of the structures was tried both with an agarose gel and with an Amicon size exclusion filter (molecular weight cutoff: 100kDa). According to general experience, the yield of purification via agarose gel is approximately 2 nM. The yield of the filter purification was definitively higher, since unexpected weak dilutions led to appropriate concentrations for TEM and fluorescence microscope. Therefore we estimate the yield to be roughly 10 nM.  
The purification of the structures was tried both with an agarose gel and with an Amicon size exclusion filter (molecular weight cutoff: 100kDa). According to general experience, the yield of purification via agarose gel is approximately 2 nM. The yield of the filter purification was definitively higher, since unexpected weak dilutions led to appropriate concentrations for TEM and fluorescence microscope. Therefore we estimate the yield to be roughly 10 nM.  
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<h1>TEM Image Analysis</h1>
<h1>TEM Image Analysis</h1>
When we inspected the structure in the TEM, we saw a spread of the arms in the uprightly orientated structures (figure 1). The magnitude of this spread seemed to be correlated to the amount of DNA binding molecules in solution.  
When we inspected the structure in the TEM, we saw a spread of the arms in the uprightly orientated structures (figure 3). The magnitude of this spread seemed to be correlated to the amount of DNA binding molecules.  


[[Image:No twist control side view.png|left |350px |thumb|Side view of BM2 without DNA-binders.]]
[[Image:No twist control side view.png|left |350px |thumb|Figure 3a Side view of BM2 without DNA-binders.]]
[[Image:Twist_EtBr_7bp_side_view.png|right|350px|thumb|Side view of BM2 with one EtBr molecule every 7bp.]]
[[Image:Twist_EtBr_7bp_side_view.png|right|350px|thumb|Figure 3b Side view of BM2 with one EtBr molecule every 7bp.]]


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