Difference between revisions of "Biomod/2011/TUM/TNT/LabbookA/list TEM pictures"

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<tr bgcolor="#f5f5f5"><td valign="top">D7</td><td valign="top">oct. 27th</td><td valign="top">lost the grid before sample preparation</td><td valign="top"></td><td valign="top"></td></tr>
 
<tr bgcolor="#f5f5f5"><td valign="top">D7</td><td valign="top">oct. 27th</td><td valign="top">lost the grid before sample preparation</td><td valign="top"></td><td valign="top"></td></tr>
 
<tr bgcolor="#f5f5f5"><td valign="top">D8</td><td valign="top">oct. 27th</td><td valign="top"><a href="http://openwetware.org/images/9/9d/20111027_theU_BM24_2D8_control024.png">BM24 (15_65) 1:10 without any DNA binders</a></td><td valign="top">oct. 27th</td><td valign="top">low sample concentration, 24 images</td></tr>
 
<tr bgcolor="#f5f5f5"><td valign="top">D8</td><td valign="top">oct. 27th</td><td valign="top"><a href="http://openwetware.org/images/9/9d/20111027_theU_BM24_2D8_control024.png">BM24 (15_65) 1:10 without any DNA binders</a></td><td valign="top">oct. 27th</td><td valign="top">low sample concentration, 24 images</td></tr>
<tr bgcolor="#f5f5f5"><td valign="top">D9</td><td valign="top">oct. 27th</td><td valign="top">BM24 (15_65) 1:10 without any DNA binders</td><td valign="top"></td><td valign="top">,stain was good but dilution slightly to low</td></tr>
+
<tr bgcolor="#f5f5f5"><td valign="top">D9</td><td valign="top">oct. 27th</td><td valign="top">BM24 (15_65) 1:10 without any DNA binders</td><td valign="top"></td><td valign="top">stain was good but dilution slightly to low</td></tr>
 
<tr bgcolor="#f5f5f5"><td valign="top">D10</td><td valign="top">oct. 28st</td><td valign="top"><a href="http://openwetware.org/images/d/d1/20111028_theU_BM24_2D10_control.png">BM24 (15_65) 1:5 without any DNA binders</a></td><td valign="top">oct. 28st</td><td valign="top"> better sample concentration than D6 and D8, 60 images</td></tr>
 
<tr bgcolor="#f5f5f5"><td valign="top">D10</td><td valign="top">oct. 28st</td><td valign="top"><a href="http://openwetware.org/images/d/d1/20111028_theU_BM24_2D10_control.png">BM24 (15_65) 1:5 without any DNA binders</a></td><td valign="top">oct. 28st</td><td valign="top"> better sample concentration than D6 and D8, 60 images</td></tr>
  

Latest revision as of 23:18, 2 November 2011

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       TUM NanU - Home   

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TEM


All dilutions of the structures were made with FOB20. For the calculation of the intercalator concentration read more in the corresponding labbook entry. Due to a limitation in webspace we only uploaded a selection of 6 pictures for each grid.

BIOMOD1 Grid Box

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<colgroup> <col width=5%> <col width=5%> <col width=42,5%> <col width=5%> <col width=42,5%> </colgroup>
grid number   prepared   annotations   observed   annotation
B2sep. 16th<a href="http://openwetware.org/images/5/56/20110921_theU_BM2.png">BM2 1:5 control without DNA-binders</a>sep. 21thfine grid
B5sep. 16th<a href="http://openwetware.org/images/9/99/20110921_theU_BM2_100uM_Spermine.png">BM2 1:5 with 100 µM spermine</a>sep. 21thstructures accumulated
C2sep. 16th<a href="http://openwetware.org/images/3/37/20110921_theU_BM2_25uM_EtBr.png">BM2 1:5 with 25 µM ethidium bromide</a>sep. 21thmassive deformation of the structures
C3sep. 16th<a href="http://openwetware.org/images/3/32/20110921_theU_BM2_75uM_EtBr.png">BM2 1:5 with 75 µM ethidium bromide</a>sep. 21thbroken grid
C4sep. 16th<a href="http://openwetware.org/images/a/af/20110921_theU_BM2_250uM_EtBr.png">BM2 1:5 with 250 µM ethidium bromide</a>sep. 21thdestroyed structures, not enough structures

</html>

BIOMOD2 Grid Box

<html>

<colgroup> <col width=5%> <col width=5%> <col width=42,5%> <col width=5%> <col width=42,5%> </colgroup>
grid number   prepared   annotations   observed   annotation
A1oct. 5th<a href="http://openwetware.org/images/c/c3/20111007_theU_BM2_1_34UM_SPERMIN_A1.png">BM2 1:20 with 1.34 µM spermine (corresponding to one molecule every 7bp)</a>oct. 7thgood stain, slightly to low dilution, 62 images
A2oct. 5th<a href="http://openwetware.org/images/7/76/20111007_theU_BM2_1_34um_spermin_a2.png">BM2 1:20 with 1.34 µM spermine (corresponding to one molecule every 7bp)</a>oct. 7thgood stain, slightly to low dilution, 63 images
A3oct. 5th<a href="http://openwetware.org/images/b/b9/20111007_theU_BM2_1_34um_spermin_a3.png">BM2 1:20 with 1.34 µM spermine (corresponding to one molecule every 7bp)</a>oct. 7thgood stain, slightly to low dilution, 60 images
A4oct. 5th<a href="http://openwetware.org/images/9/9f/20111007_theU_BM2_0_42UMSPERMIN_A4.png">BM2 1:20 with 0.42 µM spermine (corresponding to one molecule every 21bp)</a>oct. 7thgood stain, slightly to low dilution, 64 images
A5oct. 5th<a href="http://openwetware.org/images/7/7e/20111007_theU_BM2_0_24uM_Spermin_a5.png">BM2 1:20 with 0.42 µM spermine (corresponding to one molecule every 21bp)</a>oct. 7thgood stain, slightly to low dilution, 63 images
A6oct. 20thBM2 1:25 with 432 nM DAPI (corresponding to one molecule every 7bp)oct. 28stno origamis on the grid, no images
A7oct. 20thBM2 1:25 with 144 nM DAPI (corresponding to one molecule every 21bp)oct. 27thno origamis on the grid, no images
A8oct. 20thBM2 1:25 with 144 nM DAPI (corresponding to one molecule every 21bp)oct. 28thno origamis on the grid, no images
A9oct. 20th<a href="http://openwetware.org/images/b/b1/20111021_theU_BM2_2A9_control.png">BM2 1:25 without any DNA binders</a>oct. 21thgood stain and dilution, 40 images
A10oct. 20th<a href="http://openwetware.org/images/5/59/20111021_theU_BM2_2A10_control.png">BM2 1:25 without any DNA binders</a>oct. 21thgood stain and dilution, 60 images
B1oct. 5th<a href="http://openwetware.org/images/d/d0/20111012_theU_BM2_0_42um_Spermin_b1.png">BM2 1:20 with 0.42 µM spermine (corresponding to one molecule every 21bp)</a>oct. 12thgrid was fine, 60 images
B2oct. 10th<a href="http://openwetware.org/images/d/dc/20111012_theU_BM2_2_27um_Etbr_b2.png">BM2 1:40 with 2.27 µM ethidium bromide (corresponding to one molecule every 7bp)</a>oct. 12thtoo high dilution, 25 images
B3oct. 10th<a href="http://openwetware.org/images/7/7c/20111013_theU_BM2_2.27uM_EtBr_B3.png">BM2 1:40 with 2.27 µM ethidium bromide (corresponding to one molecule every 7bp)</a>oct. 13thgood stain and structure concentration, 36 images
B4oct. 10th<a href="http://openwetware.org/images/b/b5/20111014_theU_BM2_2.27uM_EtBr_B4.png">BM2 1:40 with 2.27 µM ethidium bromide (corresponding to one molecule every 7bp)</a>oct. 14thgood stain but slightly to low structure concentration, 35 images
B5oct. 10thBM2 1:40 with 0.69 µM ethidium bromide (corresponding to one molecule every 21bp)
B6oct. 20th<a href="http://openwetware.org/images/0/0a/20111021_theU_BM2_2B6_control.png">BM2 without any DNA binders, 1:25</a>oct. 21thgood stain and dilution, 66 images
B7oct. 20th<a href="http://openwetware.org/images/9/9a/20111021_theU_BM2_2B7_control.png">BM2 without any DNA binders, 1:25</a>oct. 21thgood stain and dilution, 80 images
B8oct. 20th<a href="http://openwetware.org/images/3/36/20111020_theU_BM24_2B8.png">BM24 15_65 1:25 without any DNA binders</a>oct. 20thbad stain and to high dilution
B9oct. 20thBM24 2DH3ML 1:25 without any DNA binders oct. 20thbad stain, no structures on the grid, 3 images
B10oct. 21th<a href="http://openwetware.org/images/b/ba/20111021_theU_BM24_2B10_15_65.png">BM24 15_65 1:20 without any DNA binders</a>oct. 21thgood stain but slightly to high dilution, 61 images
C1oct. 10th<a href="http://openwetware.org/images/3/33/20111014_theU_BM2_0.69uM_EtBr_C1.png">BM2 1:40 with 0.69 µM ethidium bromide (corresponding to one molecule every 21bp)</a>oct. 14thgood stain but dilution slightly to low, 32 images
C2oct. 10th<a href="http://openwetware.org/images/4/44/20111014_theU_BM2_0.69uM_EtBr_C2.png">BM2 1:40 with 0.69 µM ethidium bromide (corresponding to one molecule every 21bp)</a>oct. 14thgood stain but dilution slightly to low, 31 images
C3oct. 10thBM2 1:40 without any DNA binders
C4oct. 10thBM2 1:40 without any DNA binders
C5oct. 10thBM2 1:40 without any DNA binders
C6oct. 21th<a href="http://openwetware.org/images/8/84/20111021_theU_BM24_2C6_15_65.pnghttp://openwetware.org/images/8/84/20111021_theU_BM24_2C6_15_65.png">BM24 15_65 dilution 1:20 without any DNA binders</a>oct. 21thstain was bad and to high dilution, 17 images
C7oct. 21th<a href="http://openwetware.org/images/8/89/20111021_theU_BM24_2C7_2DH3ML.png">BM24 2DH3ML dilution 1:20 without any DNA binders </a>oct. 21thstain was good but dilution slightly to high, 70 images
C8oct. 21th<a href="http://openwetware.org/images/2/2b/20111021_theU_BM24_2C8_2D3HML.png">BM24 2DH3ML 1:20 without any DNA binders</a>oct. 21thstain was good but dilution slightly to high
C9oct. 27th<a href="http://openwetware.org/images/7/74/20111027_theU_BM2_2C9_control.png">BM2 1:25 without any DNA binders</a>oct. 27good stain but to high dilution, 60 images
C10oct. 27th<a href="http://openwetware.org/images/3/31/20111027_theU_BM2_2C10_control059.png">BM2 1:25 without any DNA binders</a>oct. 27thfine grid, 60 images
D1oct. 12th<a href="http://openwetware.org/images/3/38/20111012_theU_BM2_2_4_um_Etbr_d1.png">BM2 1:25 with 2.4 µM ethidium bromide (corresponding to one molecule every 7bp)</a>oct. 12thgood stain and dilution, 74 images
D2oct. 12th<a href="http://openwetware.org/images/0/0c/20111012_theU_BM2_0_74um_EtBr_d2.png">BM2 1:25 with 0.74 µM ethidium bromide (corresponding to one molecule every 21bp)</a>oct. 12thgood stain and dilution, 89 images, chosen for class average
D3oct. 12th<a href="http://openwetware.org/images/0/09/20111012_theU_BM2_control_d3.png">BM2 1:25without any DNA binders </a>oct. 12thgood stain and dilution, 50 images
D4oct. 14th<a href="http://openwetware.org/images/5/5c/20111020_theU_BM2_2D4_432nM_DAPI.png">BM2 1:25 with 432 µM DAPI (corresponding to one molecule every 7bp)</a>oct. 20thgood stain and structur concentration but cluster, 62 images
D5oct. 14th<a href="http://openwetware.org/images/1/18/20111020_theU_2d5_144nM_dapi.png">BM2 1:25 with 432 nM DAPI (corresponding to one molecule every 7bp)</a>oct. 20thstain and dilution was good, cluster, 61 images
D6oct. 27th<a href="http://openwetware.org/images/3/32/20111027_theU_BM24_2D6_control.png">BM24 (15_65), 1:20 diluted with FOB20</a>oct. 27thgood stain but slightly to high dilution, 22 images
D7oct. 27thlost the grid before sample preparation
D8oct. 27th<a href="http://openwetware.org/images/9/9d/20111027_theU_BM24_2D8_control024.png">BM24 (15_65) 1:10 without any DNA binders</a>oct. 27thlow sample concentration, 24 images
D9oct. 27thBM24 (15_65) 1:10 without any DNA bindersstain was good but dilution slightly to low
D10oct. 28st<a href="http://openwetware.org/images/d/d1/20111028_theU_BM24_2D10_control.png">BM24 (15_65) 1:5 without any DNA binders</a>oct. 28st better sample concentration than D6 and D8, 60 images
E1oct. 20th<a href="http://openwetware.org/images/1/18/20111101_theU_Bm2_2e1_2_4um_etbr.png">BM2 1:25 with 2.4 µM EtBr (corresponding to one molecule every 7bp)</a>oct. 26thgood dilution, bad staining, 49 images
E2oct. 20th<a href="http://openwetware.org/images/a/a7/20111026_theU_BM2_2E2_2.4uM_EtBr049.png">BM2 1:25 with 2.4 µM EtBr (corresponding to one molecule every 7bp)</a>oct. 26thgood dilution but strong background, 50 images
E3oct. 20th<a href="http://openwetware.org/images/f/f9/20111026_theU_BM2_2E3_0.74uM_EtBr059.png">BM2 1:25 with 0.74 µM EtBr (corresponding to one molecule every 21bp)</a>oct. 26thfine grid, 60 images
E4oct. 20th<a href="http://openwetware.org/images/e/ed/20111026_theU_BM2_2E4_0.74uM_EtBr049.png">BM2 1:25 with 0.74 µM EtBr (corresponding to one molecule every 21bp)</a>oct. 26thfine grid, 50 images
E5oct. 20th<a href="http://openwetware.org/images/1/14/20111026_theU_BM2_2E5_432nM_DAPI.png">BM2 1:25 with 432 nM DAPI (corresponding to one molecule every 7bp)</a>oct. 26thgood dilution but weak stain, 60 images
E6oct. 28st<a href="http://openwetware.org/images/9/94/20111028_theU_BM24_E6_control.png">BM24 (15_65), 1:5</a>oct. 28stfine grid, 413 images
E7oct. 31th<a href="http://openwetware.org/images/6/69/20111031_theU_B2_6.7uM_spermine_2E7.png">BM2 1:20 with 6.7µM spermine (corresponding to one molecule every 2bp)</a>oct. 31thgrid was fine, 112 images
E8oct. 31th<a href="http://openwetware.org/images/3/37/20111031_theU_B2_6.7uM_spermine_2E8.png">BM2 1:20 with 6.7µM spermine (corresponding to one molecule every 2bp)</a>oct. 31thexcellent grid, 80 images
E9nov. 1st<a href="http://openwetware.org/images/e/e7/20111101_theU_B2_12_1umSpermin_2E9.png">BM2 1:20 with 12.1µM spermine (corresponding to one molecule every 1.5bp)</a>nov. 1stgood grid, 111 images
E10nov. 1st<a href="http://openwetware.org/images/a/ae/20111101_theU_B2_12_1umSpermin_2E10.png">BM2 1:20 with 12.1µM spermine (corresponding to one molecule every 1.5bp)</a>nov. 1stfine grid, 131 images
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