Difference between revisions of "Biomod/2011/TUM/TNT/LabbookA/list TEM pictures"

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<tr bgcolor="#f5f5f5"><td valign="top">C4</td><td valign="top">oct. 10th</td><td valign="top">BM2 1:40 without any DNA binders</td><td valign="top">4</td><td valign="top">5</td></tr>
 
<tr bgcolor="#f5f5f5"><td valign="top">C4</td><td valign="top">oct. 10th</td><td valign="top">BM2 1:40 without any DNA binders</td><td valign="top">4</td><td valign="top">5</td></tr>
 
<tr bgcolor="#f5f5f5"><td valign="top">C5</td><td valign="top">oct. 10th</td><td valign="top">BM2 1:40 without any DNA binders</td><td valign="top">4</td><td valign="top">5</td></tr>
 
<tr bgcolor="#f5f5f5"><td valign="top">C5</td><td valign="top">oct. 10th</td><td valign="top">BM2 1:40 without any DNA binders</td><td valign="top">4</td><td valign="top">5</td></tr>
<tr bgcolor="#f5f5f5"><td valign="top">C6</td><td valign="top">oct. 21th</td><td valign="top">BM24 15_65 dilution 1:20 without any DNA binders</td><td valign="top">oct. 21th</td><td valign="top">stain was bad and to high dilution, 17 images</td></tr>
+
<tr bgcolor="#f5f5f5"><td valign="top">C6</td><td valign="top">oct. 21th</td><td valign="top"><a href="http://openwetware.org/images/8/84/20111021_theU_BM24_2C6_15_65.pnghttp://openwetware.org/images/8/84/20111021_theU_BM24_2C6_15_65.png">BM24 15_65 dilution 1:20 without any DNA binders</a></td><td valign="top">oct. 21th</td><td valign="top">stain was bad and to high dilution, 17 images</td></tr>
 
<tr bgcolor="#f5f5f5"><td valign="top">C7</td><td valign="top">oct. 21th</td><td valign="top"><a href="http://openwetware.org/images/8/89/20111021_theU_BM24_2C7_2DH3ML.png">BM24 2DH3ML dilution 1:20 without any DNA binders </a></td><td valign="top">oct. 21th</td><td valign="top">stain was good but dilution slightly to high, 70 images</td></tr>
 
<tr bgcolor="#f5f5f5"><td valign="top">C7</td><td valign="top">oct. 21th</td><td valign="top"><a href="http://openwetware.org/images/8/89/20111021_theU_BM24_2C7_2DH3ML.png">BM24 2DH3ML dilution 1:20 without any DNA binders </a></td><td valign="top">oct. 21th</td><td valign="top">stain was good but dilution slightly to high, 70 images</td></tr>
 
<tr bgcolor="#f5f5f5"><td valign="top">C8</td><td valign="top">oct. 21th</td><td valign="top"><a href="http://openwetware.org/images/2/2b/20111021_theU_BM24_2C8_2D3HML.png">BM24 2DH3ML 1:20 without any DNA binders</a></td><td valign="top">oct. 21th</td><td valign="top">stain was good but dilution slightly to high</td></tr>
 
<tr bgcolor="#f5f5f5"><td valign="top">C8</td><td valign="top">oct. 21th</td><td valign="top"><a href="http://openwetware.org/images/2/2b/20111021_theU_BM24_2C8_2D3HML.png">BM24 2DH3ML 1:20 without any DNA binders</a></td><td valign="top">oct. 21th</td><td valign="top">stain was good but dilution slightly to high</td></tr>
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<tr bgcolor="#f5f5f5"><td valign="top">D3</td><td valign="top">oct. 12th</td><td valign="top"><a href="http://openwetware.org/images/0/09/20111012_theU_BM2_control_d3.png">BM2 1:25without any DNA binders </a></td><td valign="top">oct. 12th</td><td valign="top">good stain and dilution, 50 images</td></tr>
 
<tr bgcolor="#f5f5f5"><td valign="top">D3</td><td valign="top">oct. 12th</td><td valign="top"><a href="http://openwetware.org/images/0/09/20111012_theU_BM2_control_d3.png">BM2 1:25without any DNA binders </a></td><td valign="top">oct. 12th</td><td valign="top">good stain and dilution, 50 images</td></tr>
 
<tr bgcolor="#f5f5f5"><td valign="top">D4</td><td valign="top">oct. 14th</td><td valign="top"><a href="http://openwetware.org/images/5/5c/20111020_theU_BM2_2D4_432nM_DAPI.png">BM2 1:25 with 432 µM DAPI (corresponding to one molecule every 7bp)</a></td><td valign="top">oct. 20th</td><td valign="top">good stain and structur concentration but cluster, 62 images</td></tr>
 
<tr bgcolor="#f5f5f5"><td valign="top">D4</td><td valign="top">oct. 14th</td><td valign="top"><a href="http://openwetware.org/images/5/5c/20111020_theU_BM2_2D4_432nM_DAPI.png">BM2 1:25 with 432 µM DAPI (corresponding to one molecule every 7bp)</a></td><td valign="top">oct. 20th</td><td valign="top">good stain and structur concentration but cluster, 62 images</td></tr>
<tr bgcolor="#f5f5f5"><td valign="top">D5</td><td valign="top">oct. 14th</td><td valign="top">BM2 1:25 with 432 nM DAPI (corresponding to one molecule every 7bp)</td><td valign="top">oct. 20th</td><td valign="top">stain and dilution was good, cluster, 61 images</td></tr>
+
<tr bgcolor="#f5f5f5"><td valign="top">D5</td><td valign="top">oct. 14th</td><td valign="top"><a href="http://openwetware.org/images/1/18/20111020_theU_2d5_144nM_dapi.png">BM2 1:25 with 432 nM DAPI (corresponding to one molecule every 7bp)</a></td><td valign="top">oct. 20th</td><td valign="top">stain and dilution was good, cluster, 61 images</td></tr>
<tr bgcolor="#f5f5f5"><td valign="top">D6</td><td valign="top">oct. 27th</td><td valign="top">BM24 (15_65), 1:20 diluted with FOB20</td><td valign="top">oct. 27th</td><td valign="top">good stain but slightly to high dilution, 22 images</td></tr>
+
<tr bgcolor="#f5f5f5"><td valign="top">D6</td><td valign="top">oct. 27th</td><td valign="top"><a href="http://openwetware.org/images/3/32/20111027_theU_BM24_2D6_control.png">BM24 (15_65), 1:20 diluted with FOB20</a></td><td valign="top">oct. 27th</td><td valign="top">good stain but slightly to high dilution, 22 images</td></tr>
 
<tr bgcolor="#f5f5f5"><td valign="top">D7</td><td valign="top">oct. 27th</td><td valign="top">lost the grid before sample preparation</td><td valign="top">4</td><td valign="top">5</td></tr>
 
<tr bgcolor="#f5f5f5"><td valign="top">D7</td><td valign="top">oct. 27th</td><td valign="top">lost the grid before sample preparation</td><td valign="top">4</td><td valign="top">5</td></tr>
 
<tr bgcolor="#f5f5f5"><td valign="top">D8</td><td valign="top">oct. 27th</td><td valign="top"><a href="http://openwetware.org/images/9/9d/20111027_theU_BM24_2D8_control024.png">BM24 (15_65) 1:10 without any DNA binders</a></td><td valign="top">oct. 27th</td><td valign="top">low sample concentration, 24 images</td></tr>
 
<tr bgcolor="#f5f5f5"><td valign="top">D8</td><td valign="top">oct. 27th</td><td valign="top"><a href="http://openwetware.org/images/9/9d/20111027_theU_BM24_2D8_control024.png">BM24 (15_65) 1:10 without any DNA binders</a></td><td valign="top">oct. 27th</td><td valign="top">low sample concentration, 24 images</td></tr>
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<tr bgcolor="#f5f5f5"><td valign="top">E7</td><td valign="top">oct. 31th</td><td valign="top"><a href="http://openwetware.org/images/6/69/20111031_theU_B2_6.7uM_spermine_2E7.png">BM2 1:20 with 6.7µM spermine (corresponding to one molecule every 2bp)</a></td><td valign="top">oct. 31th</td><td valign="top">grid was fine, 112 images</td></tr>
 
<tr bgcolor="#f5f5f5"><td valign="top">E7</td><td valign="top">oct. 31th</td><td valign="top"><a href="http://openwetware.org/images/6/69/20111031_theU_B2_6.7uM_spermine_2E7.png">BM2 1:20 with 6.7µM spermine (corresponding to one molecule every 2bp)</a></td><td valign="top">oct. 31th</td><td valign="top">grid was fine, 112 images</td></tr>
 
<tr bgcolor="#f5f5f5"><td valign="top">E8</td><td valign="top">oct. 31th</td><td valign="top"><a href="http://openwetware.org/images/3/37/20111031_theU_B2_6.7uM_spermine_2E8.png">BM2 1:20 with 6.7µM spermine (corresponding to one molecule every 2bp)</a></td><td valign="top">oct. 31th</td><td valign="top">excellent grid, 80 images</td></tr>
 
<tr bgcolor="#f5f5f5"><td valign="top">E8</td><td valign="top">oct. 31th</td><td valign="top"><a href="http://openwetware.org/images/3/37/20111031_theU_B2_6.7uM_spermine_2E8.png">BM2 1:20 with 6.7µM spermine (corresponding to one molecule every 2bp)</a></td><td valign="top">oct. 31th</td><td valign="top">excellent grid, 80 images</td></tr>
<tr bgcolor="#f5f5f5"><td valign="top">E9</td><td valign="top">nov. 1st</td><td valign="top">BM2 1:20 with 12.1µM spermine (corresponding to one molecule every 1.5bp)</td><td valign="top">nov. 1st</td><td valign="top">good grid, 111 images</td></tr>
+
<tr bgcolor="#f5f5f5"><td valign="top">E9</td><td valign="top">nov. 1st</td><td valign="top"><a href="http://openwetware.org/images/e/e7/20111101_theU_B2_12_1umSpermin_2E9.png">BM2 1:20 with 12.1µM spermine (corresponding to one molecule every 1.5bp)</a></td><td valign="top">nov. 1st</td><td valign="top">good grid, 111 images</td></tr>
 
<tr bgcolor="#f5f5f5"><td valign="top">E10</td><td valign="top">nov. 1st</td><td valign="top">BM2 1:20 with 12.1µM spermine (corresponding to one molecule every 1.5bp)</td><td valign="top">nov. 1st</td><td valign="top">fine grid, 131 images</td></tr>
 
<tr bgcolor="#f5f5f5"><td valign="top">E10</td><td valign="top">nov. 1st</td><td valign="top">BM2 1:20 with 12.1µM spermine (corresponding to one molecule every 1.5bp)</td><td valign="top">nov. 1st</td><td valign="top">fine grid, 131 images</td></tr>
  
 
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TEM


All dilutions of the structures were made with FOB20. For the calculation of the intercalator concentration read more in the corresponding labbook entry. Due to a limitation in webspace we only uploaded a selection of 6 pictures for each grid.

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<tr bgcolor="#f5f5f5"><td><b>grid number&nbsp;&nbsp;&nbsp;</b></td><td><b>prepared&nbsp;&nbsp;&nbsp;</b></td><td><b>annotations&nbsp;&nbsp;&nbsp;</b></td><td><b>observed&nbsp;&nbsp;&nbsp;</b></td><td><b>annotation</b></td></tr>

<tr><td></td></tr> <tr bgcolor="#f5f5f5"><td>B2</td><td>sep. 16th</td><td><a href="http://openwetware.org/images/5/56/20110921_theU_BM2.png">BM2 1:5 control without DNA-binders</a></td><td>sep. 21th</td><td>fine grid</td></tr> <tr bgcolor="#f5f5f5"><td>B5</td><td>sep. 16th</td><td><a href="http://openwetware.org/images/9/99/20110921_theU_BM2_100uM_Spermine.png">BM2 1:5 with 100 µM spermine</a></td><td>sep. 21th</td><td>structures accumulated</td></tr> <tr bgcolor="#f5f5f5"><td>C2</td><td>sep. 16th</td><td><a href="http://openwetware.org/images/3/37/20110921_theU_BM2_25uM_EtBr.png">BM2 1:5 with 25 µM ethidium bromide</a></td><td>sep. 21th</td><td>massive deformation of the structures</td></tr> <tr bgcolor="#f5f5f5"><td>C3</td><td>sep. 16th</td><td><a href="http://openwetware.org/images/3/32/20110921_theU_BM2_75uM_EtBr.png">BM2 1:5 with 75 µM ethidium bromide</a></td><td>sep. 21th</td><td>broken grid</td></tr> <tr bgcolor="#f5f5f5"><td>C4</td><td>sep. 16th</td><td><a href="http://openwetware.org/images/a/af/20110921_theU_BM2_250uM_EtBr.png">BM2 1:5 with 250 µM ethidium bromide</a></td><td>sep. 21th</td><td>destroyed structures, not enough structures</td></tr>

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<tr bgcolor="#f5f5f5"><td valign="top"><b>grid number&nbsp;&nbsp;&nbsp;</b></td><td valign="top"><b>prepared&nbsp;&nbsp;&nbsp;</b></td><td valign="top"><b>annotations&nbsp;&nbsp;&nbsp;</b></td><td valign="top"><b>observed&nbsp;&nbsp;&nbsp;</b></td><td valign="top"><b>annotation</b></td></tr>

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<tr bgcolor="#f5f5f5"><td valign="top">A1</td><td valign="top">oct. 5th</td><td valign="top"><a href="http://openwetware.org/images/c/c3/20111007_theU_BM2_1_34UM_SPERMIN_A1.png">BM2 1:20 with 1.34 µM spermine (corresponding to one molecule every 7bp)</a></td><td valign="top">oct. 7th</td><td valign="top">good stain, slightly to low dilution, 62 images</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">A2</td><td valign="top">oct. 5th</td><td valign="top"><a href="http://openwetware.org/images/7/76/20111007_theU_BM2_1_34um_spermin_a2.png">BM2 1:20 with 1.34 µM spermine (corresponding to one molecule every 7bp)</a></td><td valign="top">oct. 7th</td><td valign="top">good stain, slightly to low dilution, 63 images</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">A3</td><td valign="top">oct. 5th</td><td valign="top"><a href="http://openwetware.org/images/b/b9/20111007_theU_BM2_1_34um_spermin_a3.png">BM2 1:20 with 1.34 µM spermine (corresponding to one molecule every 7bp)</a></td><td valign="top">oct. 7th</td><td valign="top">good stain, slightly to low dilution, 60 images</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">A4</td><td valign="top">oct. 5th</td><td valign="top"><a href="http://openwetware.org/images/9/9f/20111007_theU_BM2_0_42UMSPERMIN_A4.png">BM2 1:20 with 0.42 µM spermine (corresponding to one molecule every 21bp)</a></td><td valign="top">oct. 7th</td><td valign="top">good stain, slightly to low dilution, 64 images</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">A5</td><td valign="top">oct. 5th</td><td valign="top"><a href="http://openwetware.org/images/7/7e/20111007_theU_BM2_0_24uM_Spermin_a5.png">BM2 1:20 with 0.42 µM spermine (corresponding to one molecule every 21bp)</a></td><td valign="top">oct. 7th</td><td valign="top">good stain, slightly to low dilution, 63 images</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">A6</td><td valign="top">oct. 20th</td><td valign="top">BM2 1:25 with 432 nM DAPI (corresponding to one molecule every 7bp)</td><td valign="top">oct. 28st</td><td valign="top">no origamis on the grid, no images</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">A7</td><td valign="top">oct. 20th</td><td valign="top">BM2 1:25 with 144 nM DAPI (corresponding to one molecule every 21bp)</td><td valign="top">oct. 27th</td><td valign="top">no origamis on the grid, no images</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">A8</td><td valign="top">oct. 20th</td><td valign="top">BM2 1:25 with 144 nM DAPI (corresponding to one molecule every 21bp)</td><td valign="top">oct. 28th<td valign="top">no origamis on the grid, no images</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">A9</td><td valign="top">oct. 20th</td><td valign="top"><a href="http://openwetware.org/images/b/b1/20111021_theU_BM2_2A9_control.png">BM2 1:25 without any DNA binders</a></td><td valign="top">oct. 21th</td><td valign="top">good stain and dilution, 40 images</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">A10</td><td valign="top">oct. 20th</td><td valign="top"><a href="http://openwetware.org/images/5/59/20111021_theU_BM2_2A10_control.png">BM2 1:25 without any DNA binders</a></td><td valign="top">oct. 21th</td><td valign="top">good stain and dilution, 60 images</td></tr>

<tr><td valign="top"></td></tr> <tr bgcolor="#f5f5f5"><td valign="top">B1</td><td valign="top">oct. 5th</td><td valign="top"><a href="http://openwetware.org/images/d/d0/20111012_theU_BM2_0_42um_Spermin_b1.png">BM2 1:20 with 0.42 µM spermine (corresponding to one molecule every 21bp)</a></td><td valign="top">oct. 12th</td><td valign="top">grid was fine, 60 images</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">B2</td><td valign="top">oct. 10th</td><td valign="top"><a href="http://openwetware.org/images/d/dc/20111012_theU_BM2_2_27um_Etbr_b2.png">BM2 1:40 with 2.27 µM ethidium bromide (corresponding to one molecule every 7bp)</a></td><td valign="top">oct. 12th</td><td valign="top">too high dilution, 25 images</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">B3</td><td valign="top">oct. 10th</td><td valign="top"><a href="http://openwetware.org/images/7/7c/20111013_theU_BM2_2.27uM_EtBr_B3.png">BM2 1:40 with 2.27 µM ethidium bromide (corresponding to one molecule every 7bp)</a></td><td valign="top">oct. 13th</td><td valign="top">good stain and structure concentration, 36 images</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">B4</td><td valign="top">oct. 10th</td><td valign="top"><a href="http://openwetware.org/images/b/b5/20111014_theU_BM2_2.27uM_EtBr_B4.png">BM2 1:40 with 2.27 µM ethidium bromide (corresponding to one molecule every 7bp)</a></td><td valign="top">oct. 14th</td><td valign="top">good stain but slightly to low structure concentration, 35 images</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">B5</td><td valign="top">oct. 10th</td><td valign="top">BM2 1:40 with 0.69 µM ethidium bromide (corresponding to one molecule every 21bp)</td><td valign="top">4</td><td valign="top">5</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">B6</td><td valign="top">oct. 20th</td><td valign="top"><a href="http://openwetware.org/images/0/0a/20111021_theU_BM2_2B6_control.png">BM2 without any DNA binders, 1:25</a></td><td valign="top">oct. 21th</td><td valign="top">good stain and dilution, 66 images</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">B7</td><td valign="top">oct. 20th</td><td valign="top"><a href="http://openwetware.org/images/9/9a/20111021_theU_BM2_2B7_control.png">BM2 without any DNA binders, 1:25</a></td><td valign="top">oct. 21th</td><td valign="top">good stain and dilution, 80 images</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">B8</td><td valign="top">oct. 20th</td><td valign="top"><a href="http://openwetware.org/images/3/36/20111020_theU_BM24_2B8.png">BM24 15_65 1:25 without any DNA binders</a></td><td valign="top">oct. 20th</td><td valign="top">bad stain and to high dilution</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">B9</td><td valign="top">oct. 20th</td><td valign="top">BM24 2DH3ML 1:25 without any DNA binders </td><td valign="top">oct. 20th</td><td valign="top">bad stain, no structures on the grid, 3 images</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">B10</td><td valign="top">oct. 21th</td><td valign="top"><a href="http://openwetware.org/images/b/ba/20111021_theU_BM24_2B10_15_65.png">BM24 15_65 1:20 without any DNA binders</a></td><td valign="top">oct. 21th</td><td valign="top">good stain but slightly to high dilution, 61 images</td></tr>

<tr><td valign="top"></td></tr> <tr bgcolor="#f5f5f5"><td valign="top">C1</td><td valign="top">oct. 10th</td><td valign="top"><a href="http://openwetware.org/images/3/33/20111014_theU_BM2_0.69uM_EtBr_C1.png">BM2 1:40 with 0.69 µM ethidium bromide (corresponding to one molecule every 21bp)</a></td><td valign="top">oct. 14th</td><td valign="top">good stain but dilution slightly to low, 32 images</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">C2</td><td valign="top">oct. 10th</td><td valign="top"><a href="http://openwetware.org/images/4/44/20111014_theU_BM2_0.69uM_EtBr_C2.png">BM2 1:40 with 0.69 µM ethidium bromide (corresponding to one molecule every 21bp)</a></td><td valign="top">oct. 14th</td><td valign="top">good stain but dilution slightly to low, 31 images</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">C3</td><td valign="top">oct. 10th</td><td valign="top">BM2 1:40 without any DNA binders</td><td valign="top">4</td><td valign="top">5</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">C4</td><td valign="top">oct. 10th</td><td valign="top">BM2 1:40 without any DNA binders</td><td valign="top">4</td><td valign="top">5</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">C5</td><td valign="top">oct. 10th</td><td valign="top">BM2 1:40 without any DNA binders</td><td valign="top">4</td><td valign="top">5</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">C6</td><td valign="top">oct. 21th</td><td valign="top"><a href="http://openwetware.org/images/8/84/20111021_theU_BM24_2C6_15_65.pnghttp://openwetware.org/images/8/84/20111021_theU_BM24_2C6_15_65.png">BM24 15_65 dilution 1:20 without any DNA binders</a></td><td valign="top">oct. 21th</td><td valign="top">stain was bad and to high dilution, 17 images</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">C7</td><td valign="top">oct. 21th</td><td valign="top"><a href="http://openwetware.org/images/8/89/20111021_theU_BM24_2C7_2DH3ML.png">BM24 2DH3ML dilution 1:20 without any DNA binders </a></td><td valign="top">oct. 21th</td><td valign="top">stain was good but dilution slightly to high, 70 images</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">C8</td><td valign="top">oct. 21th</td><td valign="top"><a href="http://openwetware.org/images/2/2b/20111021_theU_BM24_2C8_2D3HML.png">BM24 2DH3ML 1:20 without any DNA binders</a></td><td valign="top">oct. 21th</td><td valign="top">stain was good but dilution slightly to high</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">C9</td><td valign="top">oct. 27th</td><td valign="top"><a href="http://openwetware.org/images/7/74/20111027_theU_BM2_2C9_control.png">BM2 1:25 without any DNA binders</a></td><td valign="top">oct. 27</td><td valign="top">good stain but to high dilution, 60 images</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">C10</td><td valign="top">oct. 27th</td><td valign="top"><a href="http://openwetware.org/images/3/31/20111027_theU_BM2_2C10_control059.png">BM2 1:25 without any DNA binders</a></td><td valign="top">oct. 27th</td><td valign="top">fine grid, 60 images</td></tr>

<tr><td valign="top"></td></tr> <tr bgcolor="#f5f5f5"><td valign="top">D1</td><td valign="top">oct. 12th</td><td valign="top"><a href="http://openwetware.org/images/3/38/20111012_theU_BM2_2_4_um_Etbr_d1.png">BM2 1:25 with 2.4 µM ethidium bromide (corresponding to one molecule every 7bp)</a></td><td valign="top">oct. 12th</td><td valign="top">good stain and dilution, 74 images</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">D2</td><td valign="top">oct. 12th</td><td valign="top"><a href="http://openwetware.org/images/0/0c/20111012_theU_BM2_0_74um_EtBr_d2.png">BM2 1:25 with 0.74 µM ethidium bromide (corresponding to one molecule every 21bp)</a></td><td valign="top">oct. 12th</td><td valign="top">good stain and dilution, 89 images, chosen for class average</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">D3</td><td valign="top">oct. 12th</td><td valign="top"><a href="http://openwetware.org/images/0/09/20111012_theU_BM2_control_d3.png">BM2 1:25without any DNA binders </a></td><td valign="top">oct. 12th</td><td valign="top">good stain and dilution, 50 images</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">D4</td><td valign="top">oct. 14th</td><td valign="top"><a href="http://openwetware.org/images/5/5c/20111020_theU_BM2_2D4_432nM_DAPI.png">BM2 1:25 with 432 µM DAPI (corresponding to one molecule every 7bp)</a></td><td valign="top">oct. 20th</td><td valign="top">good stain and structur concentration but cluster, 62 images</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">D5</td><td valign="top">oct. 14th</td><td valign="top"><a href="http://openwetware.org/images/1/18/20111020_theU_2d5_144nM_dapi.png">BM2 1:25 with 432 nM DAPI (corresponding to one molecule every 7bp)</a></td><td valign="top">oct. 20th</td><td valign="top">stain and dilution was good, cluster, 61 images</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">D6</td><td valign="top">oct. 27th</td><td valign="top"><a href="http://openwetware.org/images/3/32/20111027_theU_BM24_2D6_control.png">BM24 (15_65), 1:20 diluted with FOB20</a></td><td valign="top">oct. 27th</td><td valign="top">good stain but slightly to high dilution, 22 images</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">D7</td><td valign="top">oct. 27th</td><td valign="top">lost the grid before sample preparation</td><td valign="top">4</td><td valign="top">5</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">D8</td><td valign="top">oct. 27th</td><td valign="top"><a href="http://openwetware.org/images/9/9d/20111027_theU_BM24_2D8_control024.png">BM24 (15_65) 1:10 without any DNA binders</a></td><td valign="top">oct. 27th</td><td valign="top">low sample concentration, 24 images</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">D9</td><td valign="top">oct. 27th</td><td valign="top"><a href="">BM24 (15_65) 1:10 without any DNA binders</a></td><td valign="top">4</td><td valign="top">,stain was good but dilution slightly to low</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">D10</td><td valign="top">oct. 28st</td><td valign="top"><a href="http://openwetware.org/images/d/d1/20111028_theU_BM24_2D10_control.png">BM24 (15_65) 1:5 without any DNA binders</a></td><td valign="top">oct. 28st</td><td valign="top"> better sample concentration than D6 and D8, 60 images</td></tr>

<tr><td valign="top"></td></tr> <tr bgcolor="#f5f5f5"><td valign="top">E1</td><td valign="top">oct. 20th</td><td valign="top">BM2 1:25 with 2.4 µM EtBr (corresponding to one molecule every 7bp)</td><td valign="top">oct. 26th</td><td valign="top">good dilution, bad staining, 49 images</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">E2</td><td valign="top">oct. 20th</td><td valign="top"><a href="http://openwetware.org/images/a/a7/20111026_theU_BM2_2E2_2.4uM_EtBr049.png">BM2 1:25 with 2.4 µM EtBr (corresponding to one molecule every 7bp)</a></td><td valign="top">oct. 26th</td><td valign="top">good dilution but strong background, 50 images</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">E3</td><td valign="top">oct. 20th</td><td valign="top"><a href="http://openwetware.org/images/f/f9/20111026_theU_BM2_2E3_0.74uM_EtBr059.png">BM2 1:25 with 0.74 µM EtBr (corresponding to one molecule every 21bp)</a><td valign="top">oct. 26th</td><td valign="top">fine grid, 60 images</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">E4</td><td valign="top">oct. 20th</td><td valign="top"><a href="http://openwetware.org/images/e/ed/20111026_theU_BM2_2E4_0.74uM_EtBr049.png">BM2 1:25 with 0.74 µM EtBr (corresponding to one molecule every 21bp)</a><td valign="top">oct. 26th</td><td valign="top">fine grid, 50 images</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">E5</td><td valign="top">oct. 20th</td><td valign="top"><a href="http://openwetware.org/images/1/14/20111026_theU_BM2_2E5_432nM_DAPI.png">BM2 1:25 with 432 nM DAPI (corresponding to one molecule every 7bp)/a></td><td valign="top">oct. 26th</td><td valign="top">good dilution but weak stain, 60 images</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">E6</td><td valign="top">oct. 28st</td><td valign="top"><a href="http://openwetware.org/images/9/94/20111028_theU_BM24_E6_control.png">BM24 (15_65), 1:5</a></td><td valign="top">oct. 28st</td><td valign="top">fine grid, 413 images</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">E7</td><td valign="top">oct. 31th</td><td valign="top"><a href="http://openwetware.org/images/6/69/20111031_theU_B2_6.7uM_spermine_2E7.png">BM2 1:20 with 6.7µM spermine (corresponding to one molecule every 2bp)</a></td><td valign="top">oct. 31th</td><td valign="top">grid was fine, 112 images</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">E8</td><td valign="top">oct. 31th</td><td valign="top"><a href="http://openwetware.org/images/3/37/20111031_theU_B2_6.7uM_spermine_2E8.png">BM2 1:20 with 6.7µM spermine (corresponding to one molecule every 2bp)</a></td><td valign="top">oct. 31th</td><td valign="top">excellent grid, 80 images</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">E9</td><td valign="top">nov. 1st</td><td valign="top"><a href="http://openwetware.org/images/e/e7/20111101_theU_B2_12_1umSpermin_2E9.png">BM2 1:20 with 12.1µM spermine (corresponding to one molecule every 1.5bp)</a></td><td valign="top">nov. 1st</td><td valign="top">good grid, 111 images</td></tr> <tr bgcolor="#f5f5f5"><td valign="top">E10</td><td valign="top">nov. 1st</td><td valign="top">BM2 1:20 with 12.1µM spermine (corresponding to one molecule every 1.5bp)</td><td valign="top">nov. 1st</td><td valign="top">fine grid, 131 images</td></tr>

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