Biomod/2011/Slovenia/BioNanoWizards/methafm

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     <td style="text-align: justify;">Figure 49.<span
style="font-weight: normal;"> Vid operating our atomic force

microscope</span></td>

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 </tbody>

</table> <span style="font-family: Arial;"> We deposited the sample (typically 5 &micro;l with ~5 nM DNA origami purified of excess staple strands) containing rectangles with DNA origami add-ons on a freshly cleaved mica (grade V1, Ted Pella), incubated for 60 s and then rinsed with 100 &micro;l of buffer contained in the sample and then two times with 100 &micro;l of MQ water. The sample was dried under nitrogen stream. Atomic force microscopy measurements were performed on dried samples in tapping mode on Agilent Technologies 5500 Scanning Probe Microscope using either silicon cantilevers ARROW-NCR or SSS-NCH (Nanoworld). Scan rate was generally 1 Hz and below, with engaging amplitude of 300-400 mV. </span><br

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