BioMicroCenter:Varioskan: Difference between revisions
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[[Image:BioMicroCenter_Varioskan.jpg|right]] | [[Image:BioMicroCenter_Varioskan.jpg|right]] | ||
== Varioskan Flash Reader == | == Varioskan Flash Reader == | ||
The Thermo Scientific Varioskan Flash Spectral Scanning Multimode Reader is a highly flexible plate reader capable of handling fluorometric (excitation/emission), luminometric (emission) and photometric (absorbance) measurements in 96+ well format. The Varioskan can handle a broad range of applications from reporter assays to FRET. The Varioskan is equipped with three on board fluid dispensers, incubator and shaker to allow for real time assays. Coupled with the [[BioMicroCenter:Tecan_Freedom_Evo|Tecan EVO]], the Varioskan should allow major improvements in high throughput experiment design. Currently the Varioskan is located in '''68-316'''. Please speak with ''' | The Thermo Scientific Varioskan Flash Spectral Scanning Multimode Reader is a highly flexible plate reader capable of handling fluorometric (excitation/emission), luminometric (emission) and photometric (absorbance) measurements in 96+ well format. The Varioskan can handle a broad range of applications from reporter assays to FRET. The Varioskan is equipped with three on board fluid dispensers, incubator and shaker to allow for real time assays. Coupled with the [[BioMicroCenter:Tecan_Freedom_Evo|Tecan EVO]], the Varioskan should allow major improvements in high throughput experiment design. Currently the Varioskan is located in '''68-316'''. Please speak with '''Shmulik Motola''' at the BioMicro Center about training if you wish to use the machine. | ||
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Revision as of 09:11, 14 March 2014
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Varioskan Flash Reader
The Thermo Scientific Varioskan Flash Spectral Scanning Multimode Reader is a highly flexible plate reader capable of handling fluorometric (excitation/emission), luminometric (emission) and photometric (absorbance) measurements in 96+ well format. The Varioskan can handle a broad range of applications from reporter assays to FRET. The Varioskan is equipped with three on board fluid dispensers, incubator and shaker to allow for real time assays. Coupled with the Tecan EVO, the Varioskan should allow major improvements in high throughput experiment design. Currently the Varioskan is located in 68-316. Please speak with Shmulik Motola at the BioMicro Center about training if you wish to use the machine.
Schedule time on the Varioskan
Specifications
| Fluorometry | Luminometry | Photometry | |
|---|---|---|---|
| Plate Density: | 6-1536 well | 6-1536 well 6-386 well spectral scan |
6-384 well |
| Wavelength: | Ex: 200-1000 nm Em: 270-840 nm |
360-670 nm 270-840 nm spectral scan |
200-1000 nM |
| Sensitivity: | <0.4 fmol flourescein/well | < amol ATP/well | +/- 2% or 0.003 Abs |
| Dynamic range: | > 6 decades | > 7 decades | 0-4 Abs @ 450 nm +/-2% |
Features:
- 3 onboard liquid dispensers
- 1ul – 10ml dispensing volumes
- Temperature control from ambient + 4C – 45C
- On board orbital shaker
Suggested Applications
- Apoptosis assays
- Ca2+ flux assays
- Cell proliferation
- Cellular assays
- Cytotoxicity and ADMETox
- DNA concentration
- ELISA/FIA/TRF-ELISA assays
- Enzyme kinetic studies
- Europium assays
- FRET assays
- TR-FRET assays
- BRET assays
- GPCR assays
- Ion channel assays
- Kinase assays
- Multilabel assays
- Reporter gene assays
- Signal transduction
- Tryptophan and tyrosine UV fluorescence
