Difference between revisions of "BioMicroCenter:Varioskan"

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[[Image:BioMicroCenter_Varioskan.jpg|right]]
 
[[Image:BioMicroCenter_Varioskan.jpg|right]]
 
== Varioskan Flash Reader ==
 
== Varioskan Flash Reader ==
The Thermo Sientific Varioskan Flash Spectral Scanning Multimode Reader is a highly flexible plate reader capable of handling fluorometric (excitation/emission), luminometric  (emission) and photometric (absorbance) measurements in 96+ well format. The Varioskan can handle a broad range of applications from reporter assays to FRET. The Varioskan is equipped with three on board fluid dispensers, incubator and shaker to allow for real time assays. Coupled with the [[BioMicroCenter:Tecan_Freedom_Evo|Tecan EVO]], the Varioskan should allow major improvements in high throughput experiment design. Currently the Varioskan is located in '''68-252d'''. Please speak with '''Linsday Julien''' about training if you wish to use the machine.
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The Thermo Scientific Varioskan Flash Spectral Scanning Multimode Reader is a highly flexible plate reader capable of handling fluorometric (excitation/emission), luminometric  (emission) and photometric (absorbance) measurements in 96+ well format. The Varioskan can handle a broad range of applications from reporter assays to FRET. The Varioskan is equipped with three on board fluid dispensers, incubator and shaker to allow for real time assays. Coupled with the [[BioMicroCenter:Tecan_Freedom_Evo|Tecan EVO]], the Varioskan should allow major improvements in high throughput experiment design. Currently the Varioskan is located in '''68-316'''. Please speak with '''Shmulik Matola''' at the BioMicro Center about training if you wish to use the machine.
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<br>
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'''[[BioMicroCenter:Vario_CAL|Schedule time on the Varioskan]]'''
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<br><br>
  
 
== Specifications ==
 
== Specifications ==
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* 3 onboard liquid dispensers
 
* 3 onboard liquid dispensers
 
* 1ul – 10ml dispensing volumes
 
* 1ul – 10ml dispensing volumes
* Tempurature control from 4C – 45C
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* Temperature control from ambient + 4C – 45C
 
* On board orbital shaker
 
* On board orbital shaker
  
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* Cellular assays
 
* Cellular assays
 
* Cytotoxicity and ADMETox
 
* Cytotoxicity and ADMETox
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* [[BioMicroCenter:Varioskan_DNA_Test|DNA concentration]]
 
* ELISA/FIA/TRF-ELISA assays
 
* ELISA/FIA/TRF-ELISA assays
 
* Enzyme kinetic studies
 
* Enzyme kinetic studies

Revision as of 07:50, 12 July 2012

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BioMicroCenter Varioskan.jpg

Varioskan Flash Reader

The Thermo Scientific Varioskan Flash Spectral Scanning Multimode Reader is a highly flexible plate reader capable of handling fluorometric (excitation/emission), luminometric (emission) and photometric (absorbance) measurements in 96+ well format. The Varioskan can handle a broad range of applications from reporter assays to FRET. The Varioskan is equipped with three on board fluid dispensers, incubator and shaker to allow for real time assays. Coupled with the Tecan EVO, the Varioskan should allow major improvements in high throughput experiment design. Currently the Varioskan is located in 68-316. Please speak with Shmulik Matola at the BioMicro Center about training if you wish to use the machine.


Schedule time on the Varioskan

Specifications

Fluorometry Luminometry Photometry
Plate Density: 6-1536 well 6-1536 well
6-386 well spectral scan
6-384 well
Wavelength: Ex: 200-1000 nm
Em: 270-840 nm
360-670 nm
270-840 nm spectral scan
200-1000 nM
Sensitivity: <0.4 fmol flourescein/well < amol ATP/well +/- 2% or 0.003 Abs
Dynamic range: > 6 decades > 7 decades 0-4 Abs @ 450 nm +/-2%

Features:

  • 3 onboard liquid dispensers
  • 1ul – 10ml dispensing volumes
  • Temperature control from ambient + 4C – 45C
  • On board orbital shaker

Suggested Applications

  • Apoptosis assays
  • Ca2+ flux assays
  • Cell proliferation
  • Cellular assays
  • Cytotoxicity and ADMETox
  • DNA concentration
  • ELISA/FIA/TRF-ELISA assays
  • Enzyme kinetic studies
  • Europium assays
  • FRET assays
  • TR-FRET assays
  • BRET assays
  • GPCR assays
  • Ion channel assays
  • Kinase assays
  • Multilabel assays
  • Reporter gene assays
  • Signal transduction
  • Tryptophan and tyrosine UV fluorescence