Difference between revisions of "BioMicroCenter:Protocols"

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== [[BioMicroCenter:Sequencing|ILLUMINA SEQUENCING PROTOCOLS]] ==
 
== [[BioMicroCenter:Sequencing|ILLUMINA SEQUENCING PROTOCOLS]] ==
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To access the official Illumina protocols please got to the [http://illumina.com Illumina website] and create an iCom account. Once an iCom account has been created please feel free to use the links below to download specific protocols.
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[[Media: BioMicro_Single_Read_Illumina_Primer_Seq_WorkFlow.xlsx | Illumina Single Read and Multiplexing Sequence Workflow]] <br>
  
 
<B><BIG> Cluster Station and Genome Analyzer </BIG></B>
 
<B><BIG> Cluster Station and Genome Analyzer </BIG></B>
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*[https://icom.illumina.com/Download/Summary/6OVjeOd3X0aH0rzu0JGTYQ Cluster Station Operations Guide]
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*[https://icom.illumina.com/Download/Summary/T8b3pGVc_EumaUulIvCw_w Cluster Station User Guide]
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*[https://icom.illumina.com/Download/Summary/pR7OF2lK7029XIt0-dkSdQ Genome Analyzer II User Guide]
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*[[Media:LaneByLaneConverterV3.zip|Python script for Lane-by-lane GAII recipe conversion (compatible with SCS2.10 recipes).]]
  
* [[BioMicroCenter:Priming and Single Read Protocol|Priming and Single Rread Protocol]]
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<B><BIG> ChIP-Seq </BIG></B>
* [[BioMicroCenter:Priming and Paired-End Protocol| Priming and Paired-End Protocol]]
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*[https://icom.illumina.com/Download/Summary/Yy2liOS-nEWyTUnT5w9UMA ChIP-Seq Sample Prep Guide]
  
<B><BIG> ChIP Seq </BIG></B>
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<B><BIG> mRNA-Seq </BIG></B>
* [http://openwetware.org/images/8/85/ChIP-Seq_Sample_Prep_11257047_RevA.pdf Illumina Protocol]
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*[https://icom.illumina.com/Download/Summary/rOfzT_z3S0C4de1GFDL7qQ Directional RNA-Seq Library Prep Pre-Release]
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*[https://icom.illumina.com/Download/Summary/kUyOp1caIk6Sea-x5DN6hw DSN Normalization Sample Prep Guide]
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*[https://icom.illumina.com/Download/Summary/A4zkeV3eLUOO2mzisssR1g mRNA-Seq Sample Prep Guide]
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<B><BIG> SmallRNA-Seq </BIG></B>
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*[https://icom.illumina.com/Download/Summary/MBkxLGP9Q0u_psYnP0xQCw SmallRNAv1.5 Sample Prep Guide]
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*[https://icom.illumina.com/Download/Summary/hJKJ92FSg0-ggFSSFSOnpQ SmallRNA Oligo Only Kit Sample Prep Guide]
  
 
<B><BIG> Genomic DNA </BIG></B>
 
<B><BIG> Genomic DNA </BIG></B>
* [http://openwetware.org/images/6/6c/Genomic_DNA_Sample_Prep_1003806_RevB.pdf Illumina protocol]
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*[https://icom.illumina.com/Download/Summary/5vvsqmSoGUKq8t6SBWjjyg Genomic Sample Prep]
* [http://openwetware.org/images/6/6c/Genomic_DNA_Sample_Prep_OligoOnly_1003492_Rev_A.pdf Illumina protocol (oligos only protocol)] - unlike the full kit, this kit provides only the adapters PCR primers. Users wishing to adapting current lab protocols may find this protocol more useful.
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*[https://icom.illumina.com/Download/Summary/vCFO-bin9ES31x5cPVYFmA Paired End Sample Prep Guide]
 
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*[https://icom.illumina.com/Download/Summary/ABR93AL3D0eLNaP3P-yckw Multiplexing Sample Prep Guide]
<B><BIG> Digital Gene Expression </BIG></B>
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*[https://icom.illumina.com/Download/Summary/LAJkz5z08kOWWwwsoZFjmg Mate Pair 2-5Kb Sample Prep Guide]
* [http://openwetware.org/images/4/47/GEX_NlaIII_Sample_Prep_1004240_RevA.pdf Illumina Protocol (NlaIII, full kit protocol)]
 
 
 
* [http://openwetware.org/images/e/ed/GeneExp_NlaIII_Sample_Prep_OligoOnly_1003652_Rev_A.pdf Illumina Protocol (NlaIII, oligos only protocol)]
 
 
 
* [http://openwetware.org/images/4/46/GEX_DpnII_Sample_Prep_1004241_RevA.pdf Illumina Protocol (DpnII, full kit protocol)]
 
 
 
* [http://openwetware.org/images/1/10/GeneExp_DpnII_Sample_Prep_OligoOnly_1003653_Rev_A.pdf Illumina Protocol (DpnII, olgios only protocol)]
 
 
 
 
 
<B><BIG> mRNA Seq </BIG></B>
 
* [http://openwetware.org/images/e/e5/MRNA-Seq_SamplePrep_1004898_RevA.pdf Illumina Protocol]
 
 
 
 
 
<B><BIG> Small RNA Seq </BIG></B>
 
* [http://www.umassmed.edu/uploadedFiles/nemo/Mellolab_smRNA_cloning_a_la_Tuschl_(071408).doc Tuschl Lab Protocol]
 
 
 
* [http://openwetware.org/images/b/bb/GEX_SmallRNA_Sample_Prep_1004239_RevA.pdf Illumina Protocol (full kit protocol)]
 
 
 
* [http://openwetware.org/images/b/bb/SmallRNA_Sample_Prep_OligoOnly_1003651_Rev_A.pdf Illumina Protocol (oligos only protocol)]
 
 
 
* [http://openwetware.org/images/b/bb/SmallRNA_Altv1.5_SamplePrep_15002615_RevA.pdf Illumina Protocol (Alternate v1.5 protocol)]
 
 
 
 
 
<B><BIG> Paired End </BIG></B>
 
 
 
* [http://openwetware.org/images/b/bb/Paired-End_Seq_SamplePrep_1005063_RevA.pdf Illumina Protocol]
 
 
 
* [http://openwetware.org/images/b/bb/MultiplexPE_SamplePrep_1005361_RevB.pdf Illumina Protocol (Paired End Multiplexing)]
 
  
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== [[BioMicroCenter:Microarray|AGILENT MICROARRAY PROTOCOLS]] ==
  
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* [[BioMicroCenter:Microarray|aRNA Synthesis and Labeling]] - [[Media:aRNA_labeling.docx| DOWNLOAD]] <br>
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* [[BioMicroCenter:Microarray|Hybridization]] - [[Media:hybridization.docx| DOWNLOAD]] <br>
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* [[BioMicroCenter:Microarray|Wash and Scan]] - [[Media:wash_scan.docx| DOWNLOAD]]
  
<B><BIG> Mate Paired Library </BIG></B>
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== [[BioMicroCenter:Microarray|Caliper Information]] ==
  
* [http://openwetware.org/images/b/bb/MatePair_2-5kbSamplePrep_1005363_RevB.pdf Illumina Protocol (2-5kb samples)]
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* [[BioMicroCenter:Microarray|BioRad HSP dimensions]] - [[Media:BioRad HSP dimensions Feb 2011.pdf| DOWNLOAD]]

Revision as of 11:00, 29 August 2013