BioMicroCenter:Protocols: Difference between revisions
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== [[BioMicroCenter:Sequencing|ILLUMINA SEQUENCING PROTOCOLS]] == | == [[BioMicroCenter:Sequencing|ILLUMINA SEQUENCING PROTOCOLS]] == | ||
To access the official Illumina protocols please got to the | To access the official Illumina protocols please got to the [http://illumina.com Illumina website] and create an iCom account. Once an iCom account has been created please feel free to use the links below to download specific protocols. | ||
[[Media: BioMicro_Single_Read_Illumina_Primer_Seq_WorkFlow.xlsx | Illumina Single Read and Multiplexing Sequence Workflow]] <br> | |||
<B><BIG> Cluster Station and Genome Analyzer </BIG></B> | |||
*[https://icom.illumina.com/Download/Summary/6OVjeOd3X0aH0rzu0JGTYQ Cluster Station Operations Guide] | |||
*[https://icom.illumina.com/Download/Summary/T8b3pGVc_EumaUulIvCw_w Cluster Station User Guide] | |||
*[https://icom.illumina.com/Download/Summary/pR7OF2lK7029XIt0-dkSdQ Genome Analyzer II User Guide] | |||
*[[Media:LaneByLaneConverterV2.zip|Python script for Lane-by-lane GAII recipe conversion (compatible with SCS2.10 recipes).]] | |||
<B><BIG> ChIP-Seq </BIG></B> | |||
*[https://icom.illumina.com/Download/Summary/Yy2liOS-nEWyTUnT5w9UMA ChIP-Seq Sample Prep Guide] | |||
<B><BIG> mRNA-Seq </BIG></B> | |||
*[https://icom.illumina.com/Download/Summary/rOfzT_z3S0C4de1GFDL7qQ Directional RNA-Seq Library Prep Pre-Release] | |||
*[https://icom.illumina.com/Download/Summary/kUyOp1caIk6Sea-x5DN6hw DSN Normalization Sample Prep Guide] | |||
*[https://icom.illumina.com/Download/Summary/A4zkeV3eLUOO2mzisssR1g mRNA-Seq Sample Prep Guide] | |||
<B><BIG> SmallRNA-Seq </BIG></B> | |||
*[https://icom.illumina.com/Download/Summary/MBkxLGP9Q0u_psYnP0xQCw SmallRNAv1.5 Sample Prep Guide] | |||
*[https://icom.illumina.com/Download/Summary/hJKJ92FSg0-ggFSSFSOnpQ SmallRNA Oligo Only Kit Sample Prep Guide] | |||
<B><BIG> Genomic DNA </BIG></B> | |||
*[https://icom.illumina.com/Download/Summary/5vvsqmSoGUKq8t6SBWjjyg Genomic Sample Prep] | |||
*[https://icom.illumina.com/Download/Summary/vCFO-bin9ES31x5cPVYFmA Paired End Sample Prep Guide] | |||
*[https://icom.illumina.com/Download/Summary/ABR93AL3D0eLNaP3P-yckw Multiplexing Sample Prep Guide] | |||
*[https://icom.illumina.com/Download/Summary/LAJkz5z08kOWWwwsoZFjmg Mate Pair 2-5Kb Sample Prep Guide] | |||
== [[BioMicroCenter:Microarray|AGILENT MICROARRAY PROTOCOLS]] == | == [[BioMicroCenter:Microarray|AGILENT MICROARRAY PROTOCOLS]] == | ||
| Line 13: | Line 39: | ||
* [[BioMicroCenter:Microarray|Hybridization]] - [[Media:hybridization.docx| DOWNLOAD]] <br> | * [[BioMicroCenter:Microarray|Hybridization]] - [[Media:hybridization.docx| DOWNLOAD]] <br> | ||
* [[BioMicroCenter:Microarray|Wash and Scan]] - [[Media:wash_scan.docx| DOWNLOAD]] | * [[BioMicroCenter:Microarray|Wash and Scan]] - [[Media:wash_scan.docx| DOWNLOAD]] | ||
== [[BioMicroCenter:Microarray|Caliper Information]] == | |||
* [[BioMicroCenter:Microarray|BioRad HSP dimensions]] - [[Media:BioRad HSP dimensions Feb 2011.pdf| DOWNLOAD]] | |||
Revision as of 07:18, 12 April 2013
| HOME -- | SEQUENCING -- | LIBRARY PREP -- | HIGH-THROUGHPUT -- | COMPUTING -- | OTHER TECHNOLOGY |
BioMicro Center Protocol Testing
ILLUMINA SEQUENCING PROTOCOLS
To access the official Illumina protocols please got to the Illumina website and create an iCom account. Once an iCom account has been created please feel free to use the links below to download specific protocols.
Illumina Single Read and Multiplexing Sequence Workflow
Cluster Station and Genome Analyzer
- Cluster Station Operations Guide
- Cluster Station User Guide
- Genome Analyzer II User Guide
- Python script for Lane-by-lane GAII recipe conversion (compatible with SCS2.10 recipes).
ChIP-Seq
mRNA-Seq
- Directional RNA-Seq Library Prep Pre-Release
- DSN Normalization Sample Prep Guide
- mRNA-Seq Sample Prep Guide
SmallRNA-Seq
Genomic DNA
- Genomic Sample Prep
- Paired End Sample Prep Guide
- Multiplexing Sample Prep Guide
- Mate Pair 2-5Kb Sample Prep Guide
