Difference between revisions of "BioMicroCenter:Protocols"

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(ILLUMINA SEQUENCING PROTOCOLS)
(ILLUMINA SEQUENCING PROTOCOLS)
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<B><BIG> ChIP Seq </BIG></B>
 
<B><BIG> ChIP Seq </BIG></B>
* [http://openwetware.org/images/8/85/ChIP-Seq_Sample_Prep_11257047_RevA.pdf Illumina Protocol]
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* [[Media:ChIP-Seq_Sample_Prep_11257047_RevA.pdf |Illumina Protocol]]
 
 
<B><BIG> Genomic DNA </BIG></B>
 
* [[Media:Genomic DNA Sample Prep 1003806 RevB.pdf | Illumina protocol]]
 
* [[Media:Genomic DNA Sample Prep OligoOnly 1003492 Rev A.pdf | Illumina protocol (oligos only protocol)]] - unlike the full kit, this kit provides only the adapters PCR primers. Users wishing to adapting current lab protocols may find this protocol more useful.
 
  
 
<B><BIG> Digital Gene Expression </BIG></B>
 
<B><BIG> Digital Gene Expression </BIG></B>
* [http://openwetware.org/images/4/47/GEX_NlaIII_Sample_Prep_1004240_RevA.pdf Illumina Protocol (NlaIII, full kit protocol)]
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* [[Media:GEX_NlaIII_Sample_Prep_1004240_RevA.pdf | Illumina Protocol (NlaIII, full kit protocol)]]
  
* [http://openwetware.org/images/e/ed/GeneExp_NlaIII_Sample_Prep_OligoOnly_1003652_Rev_A.pdf Illumina Protocol (NlaIII, oligos only protocol)]
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* [[Media:GeneExp_NlaIII_Sample_Prep_OligoOnly_1003652_Rev_A.pdf | Illumina Protocol (NlaIII, oligos only protocol)]]- unlike the full kit, this kit provides only the adapters PCR primers. Users wishing to adapting current lab protocols may find this protocol more useful.
  
* [http://openwetware.org/images/4/46/GEX_DpnII_Sample_Prep_1004241_RevA.pdf Illumina Protocol (DpnII, full kit protocol)]
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* [[Media:GEX_DpnII_Sample_Prep_1004241_RevA.pdf | Illumina Protocol (DpnII, full kit protocol)]]
  
* [http://openwetware.org/images/1/10/GeneExp_DpnII_Sample_Prep_OligoOnly_1003653_Rev_A.pdf Illumina Protocol (DpnII, olgios only protocol)]
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* [[Media:GeneExp_DpnII_Sample_Prep_OligoOnly_1003653_Rev_A.pdf | Illumina Protocol (DpnII, olgios only protocol)]]
  
  
 
<B><BIG> mRNA Seq </BIG></B>
 
<B><BIG> mRNA Seq </BIG></B>
* [http://openwetware.org/images/e/e5/MRNA-Seq_SamplePrep_1004898_RevA.pdf Illumina Protocol]
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* [[Media:MRNA-Seq_SamplePrep_1004898_RevA.pdf | Illumina Protocol]]
  
  
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* [http://www.umassmed.edu/uploadedFiles/nemo/Mellolab_smRNA_cloning_a_la_Tuschl_(071408).doc Tuschl Lab Protocol]
 
* [http://www.umassmed.edu/uploadedFiles/nemo/Mellolab_smRNA_cloning_a_la_Tuschl_(071408).doc Tuschl Lab Protocol]
  
* [http://openwetware.org/images/b/bb/GEX_SmallRNA_Sample_Prep_1004239_RevA.pdf Illumina Protocol (full kit protocol)]
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* [[Media:GEX_SmallRNA_Sample_Prep_1004239_RevA.pdf | Illumina Protocol (full kit protocol)]]
  
* [http://openwetware.org/images/b/bb/SmallRNA_Sample_Prep_OligoOnly_1003651_Rev_A.pdf Illumina Protocol (oligos only protocol)]
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* [[Media:SmallRNA_Sample_Prep_OligoOnly_1003651_Rev_A.pdf | Illumina Protocol (oligos only protocol)]]
  
* [http://openwetware.org/images/b/bb/SmallRNA_Altv1.5_SamplePrep_15002615_RevA.pdf Illumina Protocol (Alternate v1.5 protocol)]
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* [[Media:SmallRNA_Altv1.5_SamplePrep_15002615_RevA.pdf | Illumina Protocol (Alternate v1.5 protocol)]]
  
 +
<B><BIG> Genomic DNA </BIG></B>
 +
* [[Media:Genomic DNA Sample Prep 1003806 RevB.pdf | Generic Illumina protocol]]
  
<B><BIG> Paired End </BIG></B>
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* [[Media:Genomic DNA Sample Prep OligoOnly 1003492 Rev A.pdf | Illumina protocol (oligos only protocol)]]
 
 
* [http://openwetware.org/images/b/bb/Paired-End_Seq_SamplePrep_1005063_RevA.pdf Illumina Protocol]
 
 
 
* [http://openwetware.org/images/b/bb/MultiplexPE_SamplePrep_1005361_RevB.pdf Illumina Protocol (Paired End Multiplexing)]
 
 
 
  
 +
* [[Media:Paired-End_Seq_SamplePrep_1005063_RevA.pdf | Paired End Illumina Protocol]]
  
<B><BIG> Mate Paired Library </BIG></B>
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* [[Media:MultiplexPE_SamplePrep_1005361_RevB.pdf | Paired End Multiplexing Illumina Protocol]]
  
* [http://openwetware.org/images/b/bb/MatePair_2-5kbSamplePrep_1005363_RevB.pdf Illumina Protocol (2-5kb samples)]
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* [[Media:MatePair_2-5kbSamplePrep_1005363_RevB.pdf | Mate Pair Illumina Protocol (2-5kb samples)]]

Revision as of 13:35, 22 April 2009

BioMicroCenter-header6.jpg

HOME -- SEQUENCING -- LIBRARY PREP -- HIGH-THROUGHPUT -- COMPUTING -- OTHER TECHNOLOGY

BioMicro Center Protocol Testing

ILLUMINA SEQUENCING PROTOCOLS

Cluster Station and Genome Analyzer

ChIP Seq

Digital Gene Expression


mRNA Seq


Small RNA Seq

Genomic DNA