Difference between revisions of "BioMicroCenter:Pricing"

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{{BioMicroCenter}}
 
{{BioMicroCenter}}
== [[BioMicroCenter:Sequencing|ILLUMINA SEQUENCING]] ==
+
== SEQUENCING ==
 +
Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB''']]s will be given priority on the HiSeq.
  
=== SEQUENCING ===
+
{| border=1  
Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB''']]s will be given priority on the Genome Analyzer.
+
  ! ILLUMINA SEQUENCING
 
+
  !width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
{| border=1 align="left"
+
!width=80| MIT
  ! ILLUMINA SEQUENCING:HiSeq
+
  !width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
  !width=100| [[BioMicroCenter:CoreDeps|CORE LAB]]/MIT
+
  !width=80| unit
  !width=100| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
 
  !width=100| unit
 
 
  ! Notes
 
  ! Notes
 
  |-
 
  |-
  | colspan="5" align=center |<BR>'''HiSEQ 2000''' sequencing (minimum 100m reads/lane. Average ~160-240m reads/lane)
+
  | colspan="6" align=center |<BR>'''HiSEQ 2000''' sequencing (minimum 150m reads/lane*. Average ~160-240m reads/lane)
 
  |-
 
  |-
 
  | Single End (40nt)
 
  | Single End (40nt)
  |align="right"| $1,060
+
  |align="right"| $1,140
  |align="right"| $1,590
+
  |align="right"| $1,254
 +
|align="right"| $1,710
 
  |align="center"| per lane
 
  |align="center"| per lane
  | Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1y.
+
  | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1y.
 
  |-
 
  |-
 
  | Paired End (40+40nt)
 
  | Paired End (40+40nt)
  |align="right"| $1,575
+
  |align="right"| $1,710
  |align="right"| $2,363
+
|align="right"| $1,881
 +
  |align="right"| $2,565
 
  |align="center"| per lane
 
  |align="center"| per lane
 
  |  
 
  |  
 
  |-
 
  |-
 
  | Add'l 40nt
 
  | Add'l 40nt
  |align="right"| $300
+
  |align="right"| $330
  |align="right"| $450
+
  |align="right"| $363
 +
|align="right"| $495
 
  |align="center"| per lane
 
  |align="center"| per lane
  |   
+
  |
 +
  |-
 +
| colspan="6" align=center |<BR>'''NextSeq''' sequencing (minimum 250m reads/lane. maximum ~500m reads/lane)
 
  |-
 
  |-
  | colspan="5" align=center |<BR>'''MiSeq v2''' sequencing (minimum 8m reads/lane. maximum ~15m reads/lane)
+
| 91nt kit <BR> (eg. 40PE or 50SE)
 +
|align="right"| $2,100
 +
|align="right"| $2,310
 +
|align="right"| $3,150
 +
|align="center"| per flowcell
 +
| Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year <BR> ** THESE RUNS WILL HAVE LOW PRIORITY
 +
|-
 +
| 166nt kit <BR> (eg. 80PE or 150SE)
 +
|align="right"| $3,150
 +
|align="right"| $3,465
 +
|align="right"| $4,725
 +
|align="center"| per flowcell
 +
| Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year
 +
|-
 +
| 316nt kit <BR> (eg. 150PE or 300SE)
 +
|align="right"| $4,725
 +
|align="right"| $5,200
 +
|align="right"| $7,088
 +
|align="center"| per flowcell
 +
| Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year
 +
|-
 +
  | colspan="6" align=center |<BR>'''MiSeq v2''' sequencing (minimum 8m reads/lane. maximum ~15m reads/lane)
 
  |-
 
  |-
  | 70nt Run
+
  | 70nt Run <BR> (eg. 30PE or 70SE)
  |align="right"| $1,100
+
  |align="right"| $1,155
  |align="right"| $1,650
+
  |align="right"| $1,270
 +
|align="right"| $1,732
 
  |align="center"| per lane
 
  |align="center"| per lane
  | Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year
+
  | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year
 
  |-
 
  |-
  | 300nt Run (PE or SE)
+
  | 300nt Run <BR> (eg. 150PE)
  |align="right"| $1,300
+
  |align="right"| $1,365
  |align="right"| $1,950
+
  |align="right"| $1,500
 +
|align="right"| $2,050
 
  |align="center"| per lane
 
  |align="center"| per lane
  | Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year
+
  | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year
 
  |-
 
  |-
  | 500nt Run (PE or SE)
+
  | 500nt Run <BR> (eg. 250PE)
  |align="right"| $1,500
+
  |align="right"| $1,575
  |align="right"| $2,250
+
|align="right"| $1,732
 +
  |align="right"| $2,362
 
  |align="center"| per lane
 
  |align="center"| per lane
  | Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year
+
  | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year
 
  |-
 
  |-
  | colspan="5" align=center |<BR>'''MiSeq v3''' sequencing (minimum 8m reads/lane. Expected ~25m reads/lane) -- minimum will rise
+
  | colspan="6" align=center |<BR>'''MiSeq v3''' sequencing (minimum 15m reads/lane. Expected ~25m reads/lane)
 
  |-
 
  |-
  | 150nt Run
+
  | 150nt Run <BR> (eg. 75PE)
  |align="right"| $1,300
+
  |align="right"| $1,365
  |align="right"| $1,950
+
  |align="right"| $1,500
 +
|align="right"| $2,050
 
  |align="center"| per lane
 
  |align="center"| per lane
  | Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year
+
  | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year
 
  |-
 
  |-
  | 600nt Run (PE or SE)
+
  | 600nt Run <BR> (eg. 300PE)
  |align="right"| $1,800
+
  |align="right"| $1,890
  |align="right"| $2,700
+
  |align="right"| $2,080
 +
|align="right"| $2,835
 
  |align="center"| per lane
 
  |align="center"| per lane
  | Includes quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year
+
  | Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year
 +
|-
 +
| colspan="6" align=center |<BR>'''Additional Services'''
 +
|-
 +
| colspan="6" align=center |''These services apply to samples submitted for all platforms.''
 +
|-
 +
| Quality Control Only -only one QC is included per sequencing lane.
 +
|align="right"| $20
 +
|align="right"| $26
 +
|align="right"| $30
 +
|align="center"| per sample
 +
| Includes RT-PCR and Frag.Analyzer
 +
|-
 +
| Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling.
 +
|align="right"| $10
 +
|align="right"| $13
 +
|align="right"| $15
 +
|align="center"| per sample
 +
| Includes Fluorometric quantification and Frag.Analyzer
 +
|}
 +
 
 +
<BR><BR>
 +
Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB''']]s will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.''<BR>
 +
 
 +
== LIBRARY GENERATION ==
 +
=== SAMPLE PREPARATION - DNA ===
 +
 
 +
{| border=1
 +
! ILLUMINA Sample Preparation
 +
!width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
 +
!width=80| MIT
 +
!width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
 +
!width=160| unit
 +
!width=160| INPUT REQUIREMENT
 +
! Notes
 +
|-
 +
| colspan="4" | '''STANDARD THROUGHPUT DNA''' <BR> Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
 +
| colspan="3" style="color: blue;" | '''HIGH THROUGHPUT DNA''' <BR> Prices for individual samples. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff.
 +
|-
 +
| NEOPREP DNA <BR> * Neoprep will be retired 7/17
 +
|align="right"| $70
 +
|align="right"| $77
 +
|align="right"| $105
 +
|align="center"| per sample
 +
| >10uL of sample
 +
| Includes adapter ligation, barcoding, enrichment, and quality control (SYBR and FA). Insert sizes above 250bp.
 +
|-
 +
| Full Service Sheared DNA (SPRIWORKS)
 +
|align="right"| $130
 +
|align="right"| $143
 +
|align="right"| $195
 +
|align="center"| per sample
 +
| >10uL of sample
 +
| Includes adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
 +
|-
 +
| ''SPRI-TE use only''
 +
|align="right"| $85
 +
|align="right"| $95
 +
|align="right"| N/A
 +
|align="center"| per sample
 +
| <100uL
 +
| User must provide all primers. Samples will be returned for amplification.
 
  |-
 
  |-
  | colspan="5" align=center |<BR>'''GAII / GAIIx''' sequencing (Average ~25-40m reads/lane)
+
  | Intact Genomic DNA (NEXTERA) - 50ng input
 +
|align="right"| $150
 +
|align="right"| $165
 +
|align="right"| $225
 +
|align="center"| per sample
 +
| >10ul >10ng/uL
 +
| Includes clean up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
 
  |-
 
  |-
  | colspan="5" align=center |''GAII Services are now completely a la carte to allow us to run partial lanes. No guarantees are made on partial flowcell runs. Price includes data storage for two years but does not include quality control. PhiX lanes must be included in your purchased lanes if you want to use control normalization.''
+
  | rowspan="2" | Low Input Intact Genomic DNA (NEXTERA-XT) - 1ng input
 +
|align="right"| $100
 +
|align="right"| $110
 +
|align="right"| $150
 +
|align="center"| ''1'' Sample
 +
| >10uL
 +
| rowspan="4" | Includes tagmentation, indexing, enrichment, and quality control (SYBR and FA).  
 +
|-
 +
|align="right" style="color: blue;" | $1000
 +
|align="right" style="color: blue;" | $1100
 +
|align="right" style="color: blue;" | $1500
 +
|align="center" style="color: blue;" | ''16'' <BR>(2 column)<BR> TECAN
 +
|style="color: blue;" | >7ul at 0.2ng/ul in Axygen plate
 
  |-
 
  |-
  | 8 Lanes (SE/PE)
+
  | rowspan="2" | Low Input Intact Genomic DNA (NEXTERA-XT) - 80pg input
  |align="right"| $5,200 / $7,550
+
  |align="right" style="color: blue;" | $1500
  |align="right"| $7,800 / $11,325
+
|align="right" style="color: blue;" | $1650
  |align="center"| 1 day
+
  |align="right" style="color: blue;" | $2250
  | 24nt per day. 45nt per seq kit.
+
  |align="center" style="color: blue;" | ''96'' <BR> Mosquito
 +
  |style="color: blue;" | >5ul at 0.2ng/ul in Axygen plate
 
  |-
 
  |-
  | 4 Lanes (SE/PE)
+
  |align="right" style="color: blue;" | $3000
  |align="right"| $3,800 / $5,075
+
  |align="right" style="color: blue;" | $3300
  |align="right"| $5,700 / $7,613
+
  |align="right" style="color: blue;" | $4500
  |align="center"| 1 day
+
  |align="center" style="color: blue;" | ''384'' <BR> Mosquito
  | 36nt per day. 66nt per seq kit.
+
  |style="color: blue;" | >5ul at 0.2ng/ul in Axygen plate
 
  |-
 
  |-
  | 2 Lanes (SE/PE)
+
  |style="color: blue;" | 16S Amplicon
  |align="right"| $3,100 / $4,375
+
  |align="right" style="color: blue;" | $1,800
  |align="right"| $4,650 / $6,563
+
|align="right" style="color: blue;" | $1,980
  |align="center"| 1 day
+
  |align="right" style="color: blue;" | $2,700
  | 48nt per day. 102nt per seq kit.
+
  |align="center"|48 samples <BR>(6 columns)
 +
  |style="color: blue;" | >5ul, must amplify with Ct <20 by qPCR.
 +
|style="color: blue;" | Includes normalization, enrichment, pooling and quality control (SYBR and FA).  
 
  |-
 
  |-
  | 1 Lane (SE/PE)
+
  |style="color: blue;" | Other Amplicon
  |align="right"| $2,750 / NA
+
|align="right" style="color: blue;" | $1,440
  |align="right"| $4,125 / NA
+
  |align="right" style="color: blue;" | $1,584
  |align="center"| 1 day
+
  |align="right" style="color: blue;" | $2,160
  | 72nt per day. 140nt per seq kit.
+
  |align="center" style="color: blue;" |48 samples <BR>(6 columns)
 +
|style="color: blue;" | >5ul, must amplify with Ct <20 by qPCR.
 +
  |style="color: blue;" | Includes normalization, enrichment, pooling and quality control (SYBR and FA). <BR> Samples *must* be submitted amplified with appropriate linkers.
 
  |-
 
  |-
  | Add'l Day
+
  | colspan="7" | '''ADDITIONAL FEES''' <BR> Samples not in proper input format will be subject to these additional charges.
|align="right"| $600
 
|align="right"| $900
 
|align="center"| 1 day
 
| covers service contract and data storage requirements.
 
 
  |-
 
  |-
  | Add'l Sequencing Kit
+
  |style="color: blue;" | High Throughput Setup
  |align="right"| $1,450
+
  |align="right" style="color: blue;" | $50
  |align="right"| $2,175
+
  |align="right" style="color: blue;" | $55
  |align="center"| 1 Kit
+
|align="right" style="color: blue;" | $75
 +
  |align="center" style="color: blue;" |48 samples
 +
|  
 
  |  
 
  |  
 
  |-
 
  |-
  | colspan="5" align=center |<BR>'''Additional Services'''
+
  |Sample Cleanup
  |-
+
  |align="right"| $5
| colspan="5" align=center |''These services apply to samples submitted for all platforms.''
+
  |align="right"| $5.5
|-
+
  |align="right"| $7.5
| Sample Multiplexing
+
  |align="center"|per sample
  |align="right"| $50
+
|
  |align="right"| $75
+
  |SPRI cleanup of samples to remove impurities.  
  |align="center"| per 10 samples
 
  | Combined over the whole order. Only applies to samples combined by BMC technicians.
 
 
  |-
 
  |-
  | Add'l Repriming
+
  |style="color: blue;" |Quantification
  |align="right"| $200
+
  |align="right" style="color: blue;" | $30
  |align="right"| $300
+
  |align="right" style="color: blue;" | $33
  |align="center"| per flow cell
+
|align="right" style="color: blue;" | $45
  | Uses 6-8nt. Cost is divided by number of lanes requiring repriming. Repriming is required for standard Illumina multiplexing.
+
  |align="center" style="color: blue;" |12 samples
 +
|
 +
  |Quantification of samples (FA) prior to arraying.
 
  |-
 
  |-
| Quality Control Only
 
|align="right"| $25
 
|align="right"| $38
 
|align="center"| per sample
 
| Includes RT-PCR and BioAnalyzer
 
|}
 
<BR>
 
  
<BR>
+
|}
Prices effective 7.1.2013 <BR><BR>
+
 
Please note that samples submitted from [[BioMicroCenter:CoreDeps|'''CORE LAB''']]s will be given priority on the Hi-Seq. The BioMicro Center reserves the right to reject samples for sample preparations.
 
  
=== SAMPLE PREPARATION ===
+
=== SAMPLE PREPARATION - RNA ===
  
{| border=1 align="left"
+
{| border=1
 
  ! ILLUMINA Sample Preparation
 
  ! ILLUMINA Sample Preparation
  !width=100| [[BioMicroCenter:CoreDeps|CORE LAB]]/MIT
+
  !width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
  !width=100| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
+
!width=80| MIT
  !width=100| unit
+
  !width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
 +
  !width=160| unit
 +
!width=160| INPUT REQUIREMENT
 
  ! Notes
 
  ! Notes
 
  |-
 
  |-
  | colspan="5" | '''STARTING WITH DNA'''  
+
  | colspan="7" | '''STANDARD RNA''' <BR> Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
 +
|-
 +
| colspan="7" style="color: blue;" | '''HIGH THROUGHPUT RNA''' <BR> Prices for individual samples. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff.
 +
|-
 +
| Illumina NEOPREP <BR> * Neoprep will be retired 7/17
 +
|align="right"| $100
 +
|align="right"| $110
 +
|align="right"| $150
 +
|align="center"| per sample
 +
|
 +
| Performed using Neoprep system. <br> Requires polyA+ total RNA as input. No other inputs allowed. <BR>Includes purifying mRNA, generation of fragmented dsDNA, adaptor ligation, size selection, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer)
 
  |-
 
  |-
  | Full Service Sheared DNA (SPRIWORKS)
+
  |rowspan="2" | Standard polyA based isolation <BR> Kapa mRNA Hyperprep
 
  |align="right"| $150
 
  |align="right"| $150
 +
|align="right"| $165
 
  |align="right"| $225
 
  |align="right"| $225
 
  |align="center"| per sample
 
  |align="center"| per sample
  | Includes adapter ligation, size selection, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer).
+
| >10ul, >10ng/ul
 +
  |Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
 
  |-
 
  |-
  | ''SPRI-TE use only''
+
  |align="right" style="color: blue;" | $2000
  |align="right"| $100
+
|align="right" style="color: blue;" | $2200
  |align="right"| N/A
+
|align="right" style="color: blue;" | $3000
  |align="center"| per sample
+
|align="center" style="color: blue;" | ''24'' <br> TECAN
  | User must provide all primers. Samples will be returned for amplification.
+
| >10ul, >10ng/ul
 +
|Includes polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
 +
|-
 +
| rowspan="2"  style="color: blue;" | High Throughput 3' Digital Gene Expression (HT3DGE)
 +
|align="right" style="color: blue;" | $1200
 +
  |align="right" style="color: blue;" | $1320
 +
  |align="right" style="color: blue;" | $1800
 +
  |align="center" style="color: blue;" | ''24'' <br> TECAN
 +
| rowspan="2"  style="color: blue;" | 5ul at 4 ng/uL of RIN7+ polyA+ RNA.
 +
  | rowspan="2"  style="color: blue;" | Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
 +
|-
 +
|align="right" style="color: blue;" | $4000
 +
|align="right" style="color: blue;" | $4400
 +
|align="right" style="color: blue;" | $6000
 +
|align="center" style="color: blue;" | ''96'' <br> TECAN
 
  |-
 
  |-
  | Full Service Intact Genomic DNA (NEXTERA)
+
  |Total RNA sequencing:Mammalian <BR> Kapa Hyperprep + RiboErase
|align="right"| $150
 
 
  |align="right"| $225
 
  |align="right"| $225
 +
|align="right"| $247.5
 +
|align="right"| $337.5
 
  |align="center"| per sample
 
  |align="center"| per sample
  | Includes adapter ligation, size selection, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer).
+
| >10ul of >50ng/ul RNA
 +
  | Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.  
 
  |-
 
  |-
  | colspan="5" | '''STARTING WITH RNA'''
+
  |Total RNA sequencing:Other <BR> Kapa mRNA Hyperprep + Illumina RiboZero
 +
|align="right"| $250
 +
|align="right"| $275
 +
|align="right"| $375
 +
|align="center"| per sample
 +
| >10ul of >100ng/ul RNA
 +
| Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control. 
 
  |-
 
  |-
  | Illumina TruSeq
+
  |rowspan="4"| Low Input polyA+ <BR> Clontech SMARTseq v4 + NexteraXT
 
  |align="right"| $300
 
  |align="right"| $300
 +
|align="right"| $330
 
  |align="right"| $450
 
  |align="right"| $450
  |align="center"| per sample
+
  |align="center"| ''1'' sample
  | Preformed using TruSeq AND SPRIworks system. <br> Includes purifying mRNA, generation of fragmented dsDNA, adaptor ligation, size selection, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer).
+
  | >10ul of any input.  
 +
| rowspan="4" | Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
 
  |-
 
  |-
  |''+strand specific''
+
  |align="right" style="color: blue;" | $1600
  |align="right"| $30
+
  |align="right" style="color: blue;" | $1760
  |align="right"| $45
+
  |align="right" style="color: blue;" | $2400
  |align="center"| per sample
+
  |align="center" style="color: blue;" | ''24'' <BR> Mosquito
  | Requires 1ug of RNA. Uses the dUTP method.
+
  | style="color: blue;" | 5ul in axygen 96w plate
 
  |-
 
  |-
  | Epicenter Ribozero<br>''no polyA pulldown''
+
  |align="right" style="color: blue;" | $2500
  |align="right"| $350
+
  |align="right" style="color: blue;" | $2750
  |align="right"| $525
+
  |align="right" style="color: blue;" | $3750
  |align="center"| per sample
+
  |align="center" style="color: blue;" | ''96'' <BR> Mosquito
  | Requires 1ug of RNA. Based on Illumina Truseq chemistry
+
  | style="color: blue;" | 5ul in axygen 96w plate
 
  |-
 
  |-
  | Low Input (Clontech)
+
  |align="right" style="color: blue;" | $5000
  |align="right"| $400
+
  |align="right" style="color: blue;" | $5500
  |align="right"| $600
+
  |align="right" style="color: blue;" | $7500
  |align="center"| per sample
+
  |align="center" style="color: blue;" | ''384'' <BR> Mosquito
  | Can work on single cells
+
  | style="color: blue;" | 5ul in axygen 96w plates
 
  |-
 
  |-
  | Low Input (NuGen)
+
  |rowspan="1"| Low Input Mammalian Total <BR> Clontech ZapR Kit
  |align="right"| $430
+
|align="right"| $200
  |align="right"| $645
+
  |align="right"| $220
  |align="center"| per sample
+
  |align="right"| $300
  | Can work on degraded RNA.
+
  |align="center"| ''1'' sample
 +
  | >5ul of any input.
 +
| rowspan="1" | Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA). Single sample includes initial QC.
 
  |-
 
  |-
 
  | Tight Size Cut
 
  | Tight Size Cut
 
  |align="right"| $85
 
  |align="right"| $85
 +
|align="right"| $94
 
  |align="right"| $130
 
  |align="right"| $130
 
  |align="center"| per pool
 
  |align="center"| per pool
 +
|
 
  | +/- 40bp using PippinPrep
 
  | +/- 40bp using PippinPrep
 
  |-
 
  |-
 
  | Small RNA
 
  | Small RNA
 
  | align="right"|$275
 
  | align="right"|$275
 +
| align="right"|$303
 
  | align="right"|$413
 
  | align="right"|$413
 
  | align="center"|per sample
 
  | align="center"|per sample
  | Uses NEB small RNA kit
+
|
 +
  | Uses NEB, BIOO or QIAGEN small RNA kit
 +
|-
 +
| colspan="7" | '''ADDITIONAL FEES''' <BR> Samples not in proper input format will be subject to these additional charges.
 +
|-
 +
|style="color: blue;" | High Throughput Setup
 +
|align="right" style="color: blue;" | $50
 +
|align="right" style="color: blue;" | $55
 +
|align="right" style="color: blue;" | $75
 +
|align="center" style="color: blue;" |48 samples
 +
|
 +
|
 +
|-
 +
|Sample Cleanup
 +
|align="right"| $5
 +
|align="right"| $5.5
 +
|align="right"| $7.5
 +
|align="center"|per sample
 +
|
 +
|SPRI cleanup of samples to remove impurities.
 
  |-
 
  |-
  | colspan="5" | '''STARTING WITH CHROMATIN''' Note: User must supply antibodies.
+
|style="color: blue;" |Quantification
 +
|align="right" style="color: blue;" | $30
 +
|align="right" style="color: blue;" | $33
 +
|align="right" style="color: blue;" | $45
 +
|align="center" style="color: blue;" |12 samples
 +
|
 +
|Quantification of samples (FA) prior to arraying.
 +
|-
 +
|}
 +
 
 +
=== SAMPLE PREPARATION - CHROMATIN ===
 +
 
 +
{| border=1
 +
! ILLUMINA Sample Preparation
 +
!width=80| [[BioMicroCenter:CoreDeps|CORE LAB]]
 +
!width=80| MIT
 +
!width=80| [[BioMicroCenter:FAQ#NON_MIT_USERS|Non-MIT]]
 +
!width=80| unit
 +
! Notes
 +
|-
 +
  | colspan="6" | Note: User must supply antibodies.
 
  |-
 
  |-
 
  | Full Service ChIP-seq
 
  | Full Service ChIP-seq
 
  |align="right"| $210
 
  |align="right"| $210
 +
|align="right"| $231
 
  |align="right"| $315
 
  |align="right"| $315
 
  |align="center"| per sample
 
  |align="center"| per sample
  | Preformed using IP-Star AND SPRIworks system. <br> Includes ChIP, library contruction and barcoding.
+
  | Performed using IP-Star AND SPRIworks system. <br> Includes ChIP, library contruction and barcoding.
 
  |-
 
  |-
 
  | ''IP-Star Only''
 
  | ''IP-Star Only''
 
  |align="right"| $300
 
  |align="right"| $300
 +
|align="right"| $330
 
  |align="right"| $450
 
  |align="right"| $450
 
  |align="center"| per batch (1-8 samples)
 
  |align="center"| per batch (1-8 samples)
  | Preformed using IP-Star
+
  | Performed using IP-Star
 +
|-
 +
| Covaris Sonicator
 +
|align="right"| $15
 +
|align="right"| $20
 +
|align="right"| NA
 +
|align="center"| per hour
 +
| Training Required
 
  |-
 
  |-
 
|}
 
|}
<BR>
+
 
<BR>
 
<BR>
 
 
.
 
.
<BR>
 
<BR>
 
<BR>
 
<BR>
 
<BR>
 
  
 
== [[BioMicroCenter:MicroArrays|MICROARRAYS]] ==
 
== [[BioMicroCenter:MicroArrays|MICROARRAYS]] ==
Line 246: Line 434:
 
  |-
 
  |-
 
  | Eukaryote (3' or Exon: >20ng)
 
  | Eukaryote (3' or Exon: >20ng)
  |align="right"| $300
+
  |align="right"| $200
  |align="right"| $380
+
  |align="right"| $250
  |align="right"| $570
+
  |align="right"| $325
 
  |align="center"| per sample
 
  |align="center"| per sample
  | Using NuGEN sample labeling kit.
+
  | Using AFFYMETRIX sample labeling kit. For NuGEN reagents, please refer to 2015 pricing.
 
  |-
 
  |-
 
  | Eukaryote (<20ng)
 
  | Eukaryote (<20ng)
Line 268: Line 456:
 
  | colspan="6" | '''AGILENT PROCESSING'''
 
  | colspan="6" | '''AGILENT PROCESSING'''
 
  |-
 
  |-
  | Expression - ONE COLOR - Eukaryote
+
  | colspan="6" align=center |Agilent array services are being phased out in FY2016. Requests that must be processed on Agilent will be priced per experiment.
|align="right"| $200
 
|align="right"| $250
 
|align="right"| $375
 
|align="center"| per array
 
|
 
|-
 
| Expression - TWO COLOR - Eukaryote
 
|align="right"| $290
 
|align="right"| $340
 
|align="right"| $510
 
|align="center"| per array
 
| Includes both Cy5 and Cy3
 
|-
 
| miRNA - ONE COLOR
 
|align="right"| $260
 
|align="right"| $320
 
|align="right"| $480
 
|align="center"| per array
 
|
 
|-
 
| Prokaryote - ONE COLOR
 
|align="right"| $200
 
|align="right"| $260
 
|align="right"| $390
 
|align="center"| per array
 
|
 
 
  |-  
 
  |-  
 
  | Scanning Only
 
  | Scanning Only
Line 304: Line 466:
 
  |-  
 
  |-  
 
|}
 
|}
prices effective 7.1.2013
+
prices effective 7.1.2015
 
<br>
 
<br>
  
Line 313: Line 475:
 
  $400  - Eukaryote Exon or 3' (high density)      Human U133+ 2.0 / Mouse 430 2.0 / Human or Mouse Exon 1.0 ST
 
  $400  - Eukaryote Exon or 3' (high density)      Human U133+ 2.0 / Mouse 430 2.0 / Human or Mouse Exon 1.0 ST
 
  Full Price List:            [[Media:Affy_Arrays_081218.pdf| Affymetrix Prices]]
 
  Full Price List:            [[Media:Affy_Arrays_081218.pdf| Affymetrix Prices]]
* available at cost to academic labs only. shipping fees may be added. Non-academic labs should order their own arrays and have them shipped to the BioMicro Center.  
+
* available at cost to academic labs only. shipping fees may be added. Non-academic labs should order their own arrays and have them shipped to the BioMicro Center.
  
 
== [[BioMicroCenter:2100BioAnalyzer|AGILENT BIOANALYZER]] ==
 
== [[BioMicroCenter:2100BioAnalyzer|AGILENT BIOANALYZER]] ==
Line 331: Line 493:
 
  | AA - Preloaded plate
 
  | AA - Preloaded plate
 
  |align="right"| $30
 
  |align="right"| $30
  |align="right"|  
+
  |align="right"| N/A
 
  |align="center"| per row (12)
 
  |align="center"| per row (12)
 
  | 2ul per sample in each well using plates from BMC
 
  | 2ul per sample in each well using plates from BMC
Line 348: Line 510:
 
  |-
 
  |-
 
|}
 
|}
prices effective 7.1.2012
+
prices effective 7.1.2015
 
<br>.
 
<br>.
  
Line 360: Line 522:
 
  ! SIGN UP
 
  ! SIGN UP
 
  ! Notes
 
  ! Notes
|-
 
| colspan="6" align="center"| '''FLUIDIGM'''
 
|-
 
| Fluidigm BioMark
 
|align="right"| $150
 
|align="right"| $225
 
|align="center"| per use
 
| [[BioMicroCenter:Forms#Calenders|CALENDER]]
 
| Contact [[BioMicroCenter:People|Shmulik]]
 
|-
 
| 48.48 Dynamic Array Chip
 
|align="right"| $250
 
|align="right"| N/A
 
|align="center"| per cassette
 
| Cassettes are available in the BMC.
 
|
 
|-
 
| 96.96 Dynamic Array Chip
 
|align="right"| $700
 
|align="right"| N/A
 
|align="center"| per cassette
 
| Cassettes are available in the BMC.
 
|
 
|-
 
| WAFERGEN SMART CHIP
 
|align="right"| $150
 
|align="right"| N/A
 
|align="center"| per chip
 
| Chips available from BMC
 
| Contact [[BioMicroCenter:People|Michael]]
 
|-
 
| WAFERGEN TRAINING
 
|align="right"| $100
 
|align="right"| N/A
 
|align="center"| per chip
 
| Mandatory
 
| Contact [[BioMicroCenter:People|Michael]]
 
|- | colspan="6" align="center"| '''RT-PCR'''
 
 
  |-
 
  |-
 
  | Roche LightCycler 480
 
  | Roche LightCycler 480
 
  |align="right"| $10
 
  |align="right"| $10
  |align="right"| NA
+
  |align="right"| N/A
 
  |align="center"| per plate
 
  |align="center"| per plate
 
  | [[BioMicroCenter:Forms#Calenders|CALENDER]] *available for CORE only
 
  | [[BioMicroCenter:Forms#Calenders|CALENDER]] *available for CORE only
  | Contact [[BioMicroCenter:People|Michael]] for training
+
  | Contact [[BioMicroCenter:People|Jon Penterman]] for training
 
  |-
 
  |-
 
  | SYBR Green (KAPA/Roche)
 
  | SYBR Green (KAPA/Roche)
  |align="right"| $35
+
  |align="right"| $36
  |align="right"| NA
+
  |align="right"| N/A
 
  |align="center"| per ml
 
  |align="center"| per ml
 
  |
 
  |
Line 415: Line 539:
 
  | 96-well PLATES
 
  | 96-well PLATES
 
  |align="right"| $6
 
  |align="right"| $6
  |align="right"| NA
+
  |align="right"| N/A
 
  |align="center"| per plate
 
  |align="center"| per plate
 
  |
 
  |
Line 421: Line 545:
 
  |-
 
  |-
 
|}
 
|}
prices effective 7.1.2013
 
 
.
 
.
 
<br>
 
<br>
Line 449: Line 572:
 
  |align="center"| per session
 
  |align="center"| per session
 
  | TBA
 
  | TBA
  | Contact [[BioMicroCenter:People|Shmulik]] for training.
+
  | Contact [[BioMicroCenter:People|Jon Penterman]] for training.
 
  |-
 
  |-
 
| colspan="6" align="center"| '''PLATE READER'''
 
| colspan="6" align="center"| '''PLATE READER'''
 
  |-
 
  |-
 
  | Varioskan
 
  | Varioskan
  |align="right"| $10
+
  |align="right"| $18
  |align="right"| $15
+
  |align="right"| $27
  |align="center"| per session
+
  |align="center"| per hour
 
  | [[BioMicroCenter:Forms#Calenders|CALENDER]]
 
  | [[BioMicroCenter:Forms#Calenders|CALENDER]]
  | Quick scans (those requiring less then 30 minutes) are free of charge but may not be scheduled on the calender. Users who have large experiments have priority. Contact [[BioMicroCenter:People|Kevin]] for training  
+
  | Contact [[BioMicroCenter:People|Jon Penterman]] for training  
 
  |-
 
  |-
 
  | colspan="6" align="center"| '''ROBOTICS'''
 
  | colspan="6" align="center"| '''ROBOTICS'''
 
  |-
 
  |-
 
  | Tecan EVO 150
 
  | Tecan EVO 150
  |align="right"| $60
+
  |align="right"| $50
  |align="right"| $150
+
  |align="right"| $55
  |align="center"| per 2h block - based on scheduled time.
+
  |align="center"| per 1h block - based on scheduled time.
 
  | [[BioMicroCenter:Forms#Calenders|CALENDER]]
 
  | [[BioMicroCenter:Forms#Calenders|CALENDER]]
 
  |  
 
  |  
Line 484: Line 607:
 
  |-
 
  |-
 
  | Training
 
  | Training
  |align="right"| $100
+
  |align="right"| $50
  |align="right"| $150
+
  |align="right"| $55
 
  |align="center"| per session
 
  |align="center"| per session
 
  | Contact [[BioMicroCenter:People|Stuart]]
 
  | Contact [[BioMicroCenter:People|Stuart]]
Line 500: Line 623:
 
  |-
 
  |-
 
|}
 
|}
prices effective 7.1.2013
 
 
<br><br>
 
<br><br>
  
Line 512: Line 634:
 
  |-
 
  |-
 
  | Informatics Support
 
  | Informatics Support
  | align="right"| $70
+
  | align="right"| $75
  | align="right"| $90 / $135
+
  | align="right"| $100 / $130
 
  | align="right"| per hour
 
  | align="right"| per hour
 
  | Limited support for non-MIT users
 
  | Limited support for non-MIT users
 
  |-
 
  |-
 
  | Informatics Share
 
  | Informatics Share
  | align="right"| $960
+
  | align="right"| $1,000
 
  | align="right"| N/A
 
  | align="right"| N/A
 
  | align="right"| per 0.1FTE
 
  | align="right"| per 0.1FTE
Line 524: Line 646:
 
  |-
 
  |-
 
  | Data storage (1 year)
 
  | Data storage (1 year)
  |align="right"| $400
+
|align="right"| $280
  |align="right"| N/A
+
  |align="right"| $400 / N/A
 +
|align="center"| per TB
 +
|
 +
|-
 +
| Archival Storage (1 year - read only. Unmutable)
 +
|align="right"| $140
 +
  |align="right"| $200 / N/A
 
  |align="center"| per TB
 
  |align="center"| per TB
 
  |  
 
  |  
 
  |-
 
  |-
  | ROUS node (1 year)
+
  | Tape Archive / Recovery
  |align="right"| $400
+
  |align="right"| $60
  |align="right"| N/A
+
  |align="right"| $70
|align="center"| 8 CPU
+
  |align="center"| per tape
| Provides a priority queue for the lab on ROUS.
+
  |  
|-
 
| Spotfire License (1 year)
 
|align="right"| ''Contact Stephen Goldman''
 
|align="right"| N/A
 
  |align="center"| per user
 
  | (Limited seats available)
 
 
  |-
 
  |-
 
|}
 
|}
  
 
+
[[BioMicroCenter:PricingFY2017 | FY2017 Pricing]]<BR>
 +
[[BioMicroCenter:PricingFY2016 | FY2016 Pricing]]<BR>
 +
[[BioMicroCenter:PricingFY2015 | FY2015 Pricing]]<BR>
 
[[BioMicroCenter:PricingFY2014 | FY2014 Pricing]]<BR>
 
[[BioMicroCenter:PricingFY2014 | FY2014 Pricing]]<BR>
 
[[BioMicroCenter:PricingFY2013 | FY2013 Pricing]]<BR>
 
[[BioMicroCenter:PricingFY2013 | FY2013 Pricing]]<BR>
 
[[BioMicroCenter:PricingFY2012 | FY2012 Pricing]]<BR>
 
[[BioMicroCenter:PricingFY2012 | FY2012 Pricing]]<BR>
 
[[BioMicroCenter:PricingFY2011 | FY2011 Pricing]]
 
[[BioMicroCenter:PricingFY2011 | FY2011 Pricing]]

Latest revision as of 10:07, 26 April 2017

BioMicroCenter-header6.jpg

HOME -- SEQUENCING -- LIBRARY PREP -- HIGH-THROUGHPUT -- COMPUTING -- OTHER TECHNOLOGY

SEQUENCING

Please note that samples submitted from CORE LABs will be given priority on the HiSeq.

ILLUMINA SEQUENCING CORE LAB MIT Non-MIT unit Notes

HiSEQ 2000 sequencing (minimum 150m reads/lane*. Average ~160-240m reads/lane)
Single End (40nt) $1,140 $1,254 $1,710 per lane Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1y.
Paired End (40+40nt) $1,710 $1,881 $2,565 per lane
Add'l 40nt $330 $363 $495 per lane

NextSeq sequencing (minimum 250m reads/lane. maximum ~500m reads/lane)
91nt kit
(eg. 40PE or 50SE)
$2,100 $2,310 $3,150 per flowcell Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year
** THESE RUNS WILL HAVE LOW PRIORITY
166nt kit
(eg. 80PE or 150SE)
$3,150 $3,465 $4,725 per flowcell Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year
316nt kit
(eg. 150PE or 300SE)
$4,725 $5,200 $7,088 per flowcell Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year

MiSeq v2 sequencing (minimum 8m reads/lane. maximum ~15m reads/lane)
70nt Run
(eg. 30PE or 70SE)
$1,155 $1,270 $1,732 per lane Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year
300nt Run
(eg. 150PE)
$1,365 $1,500 $2,050 per lane Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year
500nt Run
(eg. 250PE)
$1,575 $1,732 $2,362 per lane Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year

MiSeq v3 sequencing (minimum 15m reads/lane. Expected ~25m reads/lane)
150nt Run
(eg. 75PE)
$1,365 $1,500 $2,050 per lane Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year
600nt Run
(eg. 300PE)
$1,890 $2,080 $2,835 per lane Includes final pool quality control (RT-PCR and BioAnalyzer), sequencing, genome alignment and data storage of BAM files for 1 year

Additional Services
These services apply to samples submitted for all platforms.
Quality Control Only -only one QC is included per sequencing lane. $20 $26 $30 per sample Includes RT-PCR and Frag.Analyzer
Rapid Quality Control - Use SYBR for sample quantification for pooling instead of qPCR. Only available for pooling. $10 $13 $15 per sample Includes Fluorometric quantification and Frag.Analyzer



Please note that samples submitted from CORE LABs will be given priority on all equipment. The BioMicro Center reserves the right to reject samples for any reason.

LIBRARY GENERATION

SAMPLE PREPARATION - DNA

ILLUMINA Sample Preparation CORE LAB MIT Non-MIT unit INPUT REQUIREMENT Notes
STANDARD THROUGHPUT DNA
Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
HIGH THROUGHPUT DNA
Prices for individual samples. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff.
NEOPREP DNA
* Neoprep will be retired 7/17
$70 $77 $105 per sample >10uL of sample Includes adapter ligation, barcoding, enrichment, and quality control (SYBR and FA). Insert sizes above 250bp.
Full Service Sheared DNA (SPRIWORKS) $130 $143 $195 per sample >10uL of sample Includes adapter ligation, size selection, barcoding, enrichment, and quality control (SYBR and FA).
SPRI-TE use only $85 $95 N/A per sample <100uL User must provide all primers. Samples will be returned for amplification.
Intact Genomic DNA (NEXTERA) - 50ng input $150 $165 $225 per sample >10ul >10ng/uL Includes clean up, tagmentation, indexing, enrichment, and quality control (SYBR and FA).
Low Input Intact Genomic DNA (NEXTERA-XT) - 1ng input $100 $110 $150 1 Sample >10uL Includes tagmentation, indexing, enrichment, and quality control (SYBR and FA).
$1000 $1100 $1500 16
(2 column)
TECAN
>7ul at 0.2ng/ul in Axygen plate
Low Input Intact Genomic DNA (NEXTERA-XT) - 80pg input $1500 $1650 $2250 96
Mosquito
>5ul at 0.2ng/ul in Axygen plate
$3000 $3300 $4500 384
Mosquito
>5ul at 0.2ng/ul in Axygen plate
16S Amplicon $1,800 $1,980 $2,700 48 samples
(6 columns)
>5ul, must amplify with Ct <20 by qPCR. Includes normalization, enrichment, pooling and quality control (SYBR and FA).
Other Amplicon $1,440 $1,584 $2,160 48 samples
(6 columns)
>5ul, must amplify with Ct <20 by qPCR. Includes normalization, enrichment, pooling and quality control (SYBR and FA).
Samples *must* be submitted amplified with appropriate linkers.
ADDITIONAL FEES
Samples not in proper input format will be subject to these additional charges.
High Throughput Setup $50 $55 $75 48 samples
Sample Cleanup $5 $5.5 $7.5 per sample SPRI cleanup of samples to remove impurities.
Quantification $30 $33 $45 12 samples Quantification of samples (FA) prior to arraying.


SAMPLE PREPARATION - RNA

ILLUMINA Sample Preparation CORE LAB MIT Non-MIT unit INPUT REQUIREMENT Notes
STANDARD RNA
Prices for individual samples. Repreps will typically be attempted on failed samples without charge.
HIGH THROUGHPUT RNA
Prices for individual samples. Repreps will NOT be attempted on failed samples without charge. Low volume (<=5ul) submission must be coordinated with BMC staff.
Illumina NEOPREP
* Neoprep will be retired 7/17
$100 $110 $150 per sample Performed using Neoprep system.
Requires polyA+ total RNA as input. No other inputs allowed.
Includes purifying mRNA, generation of fragmented dsDNA, adaptor ligation, size selection, barcoding, enrichment, and quality control (RT-PCR and BioAnalyzer)
Standard polyA based isolation
Kapa mRNA Hyperprep
$150 $165 $225 per sample >10ul, >10ng/ul Includes QC, polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
$2000 $2200 $3000 24
TECAN
>10ul, >10ng/ul Includes polyA isolation, generation of cDNA, library construction, indexing and quality control (SYBR + FA).
High Throughput 3' Digital Gene Expression (HT3DGE) $1200 $1320 $1800 24
TECAN
5ul at 4 ng/uL of RIN7+ polyA+ RNA. Includes generation of cDNA, library construction, indexing and library quality control. Does not include sample QC.
$4000 $4400 $6000 96
TECAN
Total RNA sequencing:Mammalian
Kapa Hyperprep + RiboErase
$225 $247.5 $337.5 per sample >10ul of >50ng/ul RNA Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
Total RNA sequencing:Other
Kapa mRNA Hyperprep + Illumina RiboZero
$250 $275 $375 per sample >10ul of >100ng/ul RNA Includes sample QC, ribosomal depletion, generation of cDNA, library construction, indexing and library quality control.
Low Input polyA+
Clontech SMARTseq v4 + NexteraXT
$300 $330 $450 1 sample >10ul of any input. Includes generation of cDNA, amplification, library construction, indexing and quality control (SYBR + FA). Single sample includes initial QC.
$1600 $1760 $2400 24
Mosquito
5ul in axygen 96w plate
$2500 $2750 $3750 96
Mosquito
5ul in axygen 96w plate
$5000 $5500 $7500 384
Mosquito
5ul in axygen 96w plates
Low Input Mammalian Total
Clontech ZapR Kit
$200 $220 $300 1 sample >5ul of any input. Includes generation of cDNA, amplification, library construction, rRNA degradation, indexing and quality control (SYBR + FA). Single sample includes initial QC.
Tight Size Cut $85 $94 $130 per pool +/- 40bp using PippinPrep
Small RNA $275 $303 $413 per sample Uses NEB, BIOO or QIAGEN small RNA kit
ADDITIONAL FEES
Samples not in proper input format will be subject to these additional charges.
High Throughput Setup $50 $55 $75 48 samples
Sample Cleanup $5 $5.5 $7.5 per sample SPRI cleanup of samples to remove impurities.
Quantification $30 $33 $45 12 samples Quantification of samples (FA) prior to arraying.

SAMPLE PREPARATION - CHROMATIN

ILLUMINA Sample Preparation CORE LAB MIT Non-MIT unit Notes
Note: User must supply antibodies.
Full Service ChIP-seq $210 $231 $315 per sample Performed using IP-Star AND SPRIworks system.
Includes ChIP, library contruction and barcoding.
IP-Star Only $300 $330 $450 per batch (1-8 samples) Performed using IP-Star
Covaris Sonicator $15 $20 NA per hour Training Required

.

MICROARRAYS

Microarray processing prices do not include the cost of the array. In order to be sure we have arrays on hand, we encourage users to contact us several days in advance of submitting samples.

MICROARRAYS CORE LAB MIT Non-MIT unit Notes
AFFYMETRIX PROCESSING
Eukaryote (3' or Exon: >20ng) $200 $250 $325 per sample Using AFFYMETRIX sample labeling kit. For NuGEN reagents, please refer to 2015 pricing.
Eukaryote (<20ng) $400 $500 $750 per sample Using NuGEN sample labeling kit.
Prokaryote $365 $455 $683 per sample Using Ambion sample labeling kit.
AGILENT PROCESSING
Agilent array services are being phased out in FY2016. Requests that must be processed on Agilent will be priced per experiment.
Scanning Only $50 $50 N/A per slide Price per glass slide.

prices effective 7.1.2015

Common Affymetrix Arrays* (some may be in stock):

$175  - Prokaryote/Small Eukaryote                E.coli Genome 
$250  - Eukaryote 3' (low density)                Human U133A 2.0 / Mouse 430A 2.0
$400  - Eukaryote Exon or 3' (high density)       Human U133+ 2.0 / Mouse 430 2.0 / Human or Mouse Exon 1.0 ST
Full Price List:             Affymetrix Prices
  • available at cost to academic labs only. shipping fees may be added. Non-academic labs should order their own arrays and have them shipped to the BioMicro Center.

AGILENT BIOANALYZER

AGILENT BIOANALYZER / ADVANCED ANALYTICAL-FRAGMENT ANALYZER CORE LAB / MIT Non-MIT unit Notes
RNA / DNA on Advanced Analytical $8 $12 per sample
AA - Preloaded plate $30 N/A per row (12) 2ul per sample in each well using plates from BMC
All DNA & RNA on BIOANALYZER $15 $25 per sample
High Sensitivity protein $40 $60 per sample detection of proteins at < 30ng/ul.

prices effective 7.1.2015
.

REAL TIME PCR

OTHER LAB SERVICES CORE LAB / MIT Non-MIT unit SIGN UP Notes
Roche LightCycler 480 $10 N/A per plate CALENDER *available for CORE only Contact Jon Penterman for training
SYBR Green (KAPA/Roche) $36 N/A per ml Available for MIT only
96-well PLATES $6 N/A per plate Available for MIT only

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OTHER LAB SERVICES

OTHER LAB SERVICES CORE LAB / MIT Non-MIT unit SIGN UP Notes
PIPPIN PREP
Gel Isolation $50 $75 per gel (5 lanes) TBA
Training $25 $50 per session TBA Contact Jon Penterman for training.
PLATE READER
Varioskan $18 $27 per hour CALENDER Contact Jon Penterman for training
ROBOTICS
Tecan EVO 150 $50 $55 per 1h block - based on scheduled time. CALENDER
non-filter tips $6.00 $6.00 per box ** Please let us know if there is additional plasticware you would like us to stock.
Filter tips $8.00 $8.00 per box
Training $50 $55 per session Contact Stuart
NANODROP
Nanodrop -- -- Walk up service * no charge



COMPUTATIONAL SERVICES

COMPUTATIONAL SERVICES CORE LAB Other MIT / Non-MIT unit Notes
Informatics Support $75 $100 / $130 per hour Limited support for non-MIT users
Informatics Share $1,000 N/A per 0.1FTE MIT only. Priority access to informatics.
Data storage (1 year) $280 $400 / N/A per TB
Archival Storage (1 year - read only. Unmutable) $140 $200 / N/A per TB
Tape Archive / Recovery $60 $70 per tape

FY2017 Pricing
FY2016 Pricing
FY2015 Pricing
FY2014 Pricing
FY2013 Pricing
FY2012 Pricing
FY2011 Pricing