April 19, 2010
Starting next month, the Koch Institute and the MIT BioMicro Center will be hosting a Nanostring nCounter system on a trial basis. The nCounter system is a single molecule visualizer that quantitatively detects RNA and other nucleic acids using a hybridization system in a highly multiplex manner. The system uses color-coded molecular barcodes to digitally count nucleic acid molecules in solution. The system does not use enzymes for detecting the nucleic acids, allowing direct measurements of mRNA from a variety of input materials including degraded RNA or crude cell lysates.
The instruments will be located in the BioMicro Center and will be available to all members of the MIT community. We will be using this time to gauge the level of interest in the nCounter and whether it would be a viable system to purchase.
If you are interested in learning more, we will be hosting a seminar with Nanostring representatives on April 30th. The seminar will be at 11am in 68-181. Nanostring representatives will be available after the talk to discuss the instruments and specific applications. More information is in the ABSTRACT for the talk.
April 1, 2010
Thanks to the generosity of a grant from the Moore Foundation to Dr. Penny Chisholm and ARRA funding to Dr. Chris Burge the BioMicro Center will be significantly expanding our sequencing capacity over the next 4-6 months. The first step of this expansion begins today with the addition of a new GAIIx and cBot from Illumina. We are working hard to get "Ryland" and "Agustus Gloop" up and running and we hope to be able to shorten our queue times very soon!
Due to an increase in the cost of Illumina reagents, increase in data file size, and the complexity of sample preps, we have had to increase, slightly, the cost of Illumina sequencing. These new rates will be effective on samples submitted after April 1, 2010. Samples already in the queue will use the current rates.
| ILLUMINA SEQUENCING
|| MIT - current
|| MIT - April 1, 2010
| Single End (36nt)
|| per lane
|| inlcudes quality control (RT-PCR or BioAnalyzer), sequencing, genome alignment and data storage of Firecrest files for 2 yrs.
| Paired End (36+36nt)
|| per lane
| Add'l 36nt
|| per lane
| Sample Multiplexing
|| per 10 samples combined
|| Combined over the whole order. Only applies to samples combined by BCM technicians.
|| per flow cell
|| Uses 6nt. Cost is divided by number of lanes requiring repriming. Repriming is required for standard Illumina multiplexing.
Non-MIT rates are 30% higher for academic institutions and 50% highers for non-academic institutions. Priority is given to samples from CORE facilities.