Difference between revisions of "BioMicroCenter:News"

From OpenWetWare
Jump to: navigation, search
(April 2010)
Line 4: Line 4:
 
{|
 
{|
 
|rowspan=2 valign=top style="width:55%;padding-right:10px;"|  
 
|rowspan=2 valign=top style="width:55%;padding-right:10px;"|  
 +
 +
=== April 19, 2010 ===
 +
 +
Starting next month, the [http://mit.edu/ki/index.html Koch Institute] and the MIT BioMicro Center will be hosting a [http://www.nanostring.com/ Nanostring] nCounter system on a trial basis. The [http://www.nanostring.com/products/ncounter/ nCounter system] is a [http://www.nanostring.com/applications/technology/ single molecule visualizer] that quantitatively detects RNA and other nucleic acids using a hybridization system in a highly multiplex manner. The system uses color-coded molecular barcodes to digitally count nucleic acid molecules in solution. The system does not use enzymes for detecting the nucleic acids, allowing direct measurements of mRNA from a variety of input materials including degraded RNA or crude cell lysates.
 +
 +
The instruments will be located in the BioMicro Center and will be available to all members of the MIT community. We will be using this time to gauge the level of interest in the nCounter and whether it would be a viable system to purchase.
 +
 +
If you are interested in learning more, we will be hosting a seminar with [http://www.nanostring.com/ Nanostring] representatives on April 30th. The seminar will be at 11am in 68-181. Nanostring representatives will be available after the talk to discuss the instruments and specific applications. More information is in the ABSTRACT for the talk.
 +
  
 
=== April 1, 2010 ===
 
=== April 1, 2010 ===

Revision as of 17:12, 19 April 2010

BioMicroCenter-header6.jpg

<html> <style type="text/css">

  1. menuh-container{

position: absolute; top: 1em; left: 1em; }

  1. menuh{

font-family: arial, helvetica, sans-serif; width:100%; float:left; margin:2em; margin-top: 1em; }

  1. menuh top_parent{

/* border-style: none; */ }

  1. menuh a{

text-align: center; display:block; white-space:nowrap; margin:0; padding: 0.3em; }

  1. menuh a:link, #menuh a:visited, #menuh a:active{ /* menu at rest */

color: black; background-color: white; text-decoration:none; }

  1. menuh a:hover{ /* menu at mouse-over */

color: black; background-color: #f0f0d1; text-decoration:none; }

  1. menuh a.top_parent, #menuh a.top_parent:hover{ /* attaches down-arrow to all top-parents */

font-size: 10pt; background-image: url(navdown_white.gif); background-position: right center; background-repeat: no-repeat; }

  1. menuh a.parent, #menuh a.parent:hover{ /* attaches side-arrow to all parents */

font-size: 10pt; background-image: url(nav_white.gif); background-position: right center; background-repeat: no-repeat; }

  1. menuh ul{

font-size: 8pt; font-family: arial, helvetica, sans-serif; list-style:none; margin:0; padding:0; float:left; width:13em; /* width of all menu boxes */ }

  1. menuh li{

position:relative; min-height: 1px; /* Sophie Dennis contribution for IE7 vertical-align: bottom; /* Sophie Dennis contribution for IE7 */ }

  1. menuh ul ul{

position:absolute; z-index:500; top:auto; display:none; padding: 1em; margin:-1em 0 0 -1em; }

  1. menuh ul ul ul{

top:0; left:100%; } div#menuh li:hover{ cursor:pointer; z-index:100; } div#menuh li:hover ul ul, div#menuh li li:hover ul ul, div#menuh li li li:hover ul ul, div#menuh li li li li:hover ul ul {display:none;}

div#menuh li:hover ul, div#menuh li li:hover ul, div#menuh li li li:hover ul, div#menuh li li li li:hover ul {display:block;}

/* End CSS Drop Down Menu */ </style> <style type="text/css" media="screen">

  1. menuh-container{

font-size: 1em; position: relative; top:0; left: 5%; width: 90%; margin: 0px; border: 1px solid white; } </style>

</html>


April 19, 2010

Starting next month, the Koch Institute and the MIT BioMicro Center will be hosting a Nanostring nCounter system on a trial basis. The nCounter system is a single molecule visualizer that quantitatively detects RNA and other nucleic acids using a hybridization system in a highly multiplex manner. The system uses color-coded molecular barcodes to digitally count nucleic acid molecules in solution. The system does not use enzymes for detecting the nucleic acids, allowing direct measurements of mRNA from a variety of input materials including degraded RNA or crude cell lysates.

The instruments will be located in the BioMicro Center and will be available to all members of the MIT community. We will be using this time to gauge the level of interest in the nCounter and whether it would be a viable system to purchase.

If you are interested in learning more, we will be hosting a seminar with Nanostring representatives on April 30th. The seminar will be at 11am in 68-181. Nanostring representatives will be available after the talk to discuss the instruments and specific applications. More information is in the ABSTRACT for the talk.


April 1, 2010

Thanks to the generosity of a grant from the Moore Foundation to Dr. Penny Chisholm and ARRA funding to Dr. Chris Burge the BioMicro Center will be significantly expanding our sequencing capacity over the next 4-6 months. The first step of this expansion begins today with the addition of a new GAIIx and cBot from Illumina. We are working hard to get "Ryland" and "Agustus Gloop" up and running and we hope to be able to shorten our queue times very soon!

March 2010

Due to an increase in the cost of Illumina reagents, increase in data file size, and the complexity of sample preps, we have had to increase, slightly, the cost of Illumina sequencing. These new rates will be effective on samples submitted after April 1, 2010. Samples already in the queue will use the current rates.

ILLUMINA SEQUENCING MIT - current MIT - April 1, 2010 unit Notes
Single End (36nt) $805 $860 per lane inlcudes quality control (RT-PCR or BioAnalyzer), sequencing, genome alignment and data storage of Firecrest files for 2 yrs.
Paired End (36+36nt) $1,385 $1,450 per lane
Add'l 36nt $295 $300 per lane
Sample Multiplexing $0 $50 per 10 samples combined Combined over the whole order. Only applies to samples combined by BCM technicians.
Repriming $200 $300 per flow cell Uses 6nt. Cost is divided by number of lanes requiring repriming. Repriming is required for standard Illumina multiplexing.


Non-MIT rates are 30% higher for academic institutions and 50% highers for non-academic institutions. Priority is given to samples from CORE facilities.


RECENT & UPCOMING CHANGES

Illumina/Solexa Sequencing -- *New*
New Affymetrix labeling -- *New*
Notes from the ABRF
Tecan EVO high-throughput -- Training Feb 12, 2009
BioMicro Center Renovation -- Coming Feb '09


PREVIOUS NEWSLETTERS

2009
May
January / February
2008
December
November


RECENT CHANGES TO THE WEBSITE <\BIG><\B>

26 September 2017

     04:06 

Butlin:Unix for Bioinformatics - advanced tutorial‎‎ (3 changes | history) . . (+334). . [Claudius E Kerth‎ (3×)]

     

04:06

(cur | prev) . . (+148). . Claudius E Kerth (talk | contribs) (Added troublehooting tip: when rename.pl download link does not work, go to my Dropbox)

     

02:44

(cur | prev) . . (+168). . Claudius E Kerth (talk | contribs) (added Troubleshooting tip)

 m   

02:36

(cur | prev) . . (+18). . Claudius E Kerth (talk | contribs) (TASK 5: I have split my reads by barcode and I have quality filtered them. Now I want to know how many reads I have left from each (barcoded) individual. How can I find that out?)

25 September 2017

     21:19 

BME100 f2017:Group12 W0800 L3‎‎ (12 changes | history) . . (+3,361). . [Priscilla Y. Han‎ (12×)]

     

21:19

(cur | prev) . . (+25). . Priscilla Y. Han (talk | contribs) (Descriptive Stats and Graph)

     

21:15

(cur | prev) . . (+70). . Priscilla Y. Han (talk | contribs) (Descriptive Stats and Graph)

     

21:11

(cur | prev) . . (-3). . Priscilla Y. Han (talk | contribs) (Experimental Design of Device)

     

21:10

(cur | prev) . . (-12). . Priscilla Y. Han (talk | contribs) (Experimental Design of Device)

     

21:10

(cur | prev) . . (+15). . Priscilla Y. Han (talk | contribs) (Experimental Design of Device)

     

21:09

(cur | prev) . . (+3,181). . Priscilla Y. Han (talk | contribs) (Experimental Design of Device)

     

21:05

(cur | prev) . . (+5). . Priscilla Y. Han (talk | contribs) (Descriptive Stats and Graph)

     

21:03

(cur | prev) . . (+25). . Priscilla Y. Han (talk | contribs) (Descriptive Stats and Graph)

     

21:01

(cur | prev) . . (+2). . Priscilla Y. Han (talk | contribs) (Descriptive Stats and Graph)

     

20:58

(cur | prev) . . (+29). . Priscilla Y. Han (talk | contribs) (Descriptive Stats and Graph)

     

20:57

(cur | prev) . . (-3). . Priscilla Y. Han (talk | contribs) (Descriptive Stats and Graph)

     

20:55

(cur | prev) . . (+27). . Priscilla Y. Han (talk | contribs) (Descriptive Stats and Graph)

     21:19 

(Upload log). .

[Ana C. Carr‎ (4×); Priscilla Y. Han‎ (7×)]

     

21:19

. . Priscilla Y. Han (talk | contribs) uploaded File:Pulsingbargraph12.png

     

21:18

. . Priscilla Y. Han (talk | contribs) uploaded File:Mypear sonheart12.png

     

21:18

. . Priscilla Y. Han (talk | contribs) uploaded File:Heartratespree12.png

     

21:04

. . Priscilla Y. Han (talk | contribs) uploaded File:Scrnshotspree12.png

     

20:59

. . Priscilla Y. Han (talk | contribs) uploaded File:2columnbarsspree.png

     

20:56

. . Priscilla Y. Han (talk | contribs) uploaded a new version of File:Mypear sonr.png

     

20:56

. . Priscilla Y. Han (talk | contribs) uploaded File:Mypear sonr.png

     

18:36

. . Ana C. Carr (talk | contribs) uploaded a new version of File:IMG 5562.jpg(In action)

     

18:36

. . Ana C. Carr (talk | contribs) uploaded a new version of File:IMG 5562.jpg(In action)

     

18:36

. . Ana C. Carr (talk | contribs) uploaded a new version of File:IMG 5562.jpg(In action)

     

18:36

. . Ana C. Carr (talk | contribs) uploaded a new version of File:IMG 5562.jpg(In action)

     20:33 

BME100 f2017:Group8 W1030 L3‎‎ (9 changes | history) . . (+501). . [Jennifer L. Brodsky‎ (9×)]

     

20:33

(cur | prev) . . (+5). . Jennifer L. Brodsky (talk | contribs) (Experimental Design of Own Device)

     

20:32

(cur | prev) . . (+112). . Jennifer L. Brodsky (talk | contribs) (Experimental Design of Own Device)

     

20:29

(cur | prev) . . (0). . Jennifer L. Brodsky (talk | contribs) (Experimental Design of Own Device)

     

20:28

(cur | prev) . . (+13). . Jennifer L. Brodsky (talk | contribs) (Experimental Design of Own Device)

     

20:28

(cur | prev) . . (-1). . Jennifer L. Brodsky (talk | contribs) (Experimental Design of Own Device)

     

20:26

(cur | prev) . . (+1). . Jennifer L. Brodsky (talk | contribs) (Experimental Design of Own Device)

     

20:26

(cur | prev) . . (+177). . Jennifer L. Brodsky (talk | contribs) (Experimental Design of Own Device)

     

20:25

(cur | prev) . . (+7). . Jennifer L. Brodsky (talk | contribs) (Experimental Design of Own Device)

     

20:24

(cur | prev) . . (+187). . Jennifer L. Brodsky (talk | contribs) (Experimental Design of Own Device)

N    20:17  BME100 f2017:Group10 W0800 L3‎ (diff | hist) . . (+767). . Whitney Hirano (talk | contribs) (Created page with "5. The mean value of heart rate calculated from the pulse ox was 98.3673 beats/min with a standard deviation of 22.2273, while the mean value of heart rate calculated from the...")
     19:56  BME100 f2017:Group9 W0800 L3‎ (diff | hist) . . (+3). . Irene Zhang (talk | contribs) (LAB 3 WRITE-UP)

     19:52 

BME100 f2017:Projects1‎‎ (3 changes | history) . . (+19). . [Yar‎; Karmella Haynes‎ (2×)]

     

19:52

(cur | prev) . . (0). . Yar (talk | contribs) (small change to force cache purge)

     

19:37

(cur | prev) . . (+21). . Karmella Haynes (talk | contribs)

     

19:11

(cur | prev) . . (-2). . Karmella Haynes (talk | contribs) (Lab 1: “Needs Identification”)

     19:19  CONJ606:Schedule‎ (diff | hist) . . (+23). . Maureen E. Hoatlin (talk | contribs) (Week-by-Week Schedule Summary)

     19:06 

BME100 f2017‎‎ (2 changes | history) . . (+1). . [Karmella Haynes‎ (2×)]

     

19:06

(cur | prev) . . (-8). . Karmella Haynes (talk | contribs)

     

19:06

(cur | prev) . . (+9). . Karmella Haynes (talk | contribs)

     17:38  User:Oww Bot‎ (diff | hist) . . (-956). . Yar (talk | contribs) (it me)
     17:36 (User rights log) . . Yar (talk | contribs) changed group membership for Oww Bot from bot and administrator to bot ‎
     17:35  User:Yar‎ (diff | hist) . . (+26). . Yar (talk | contribs) (my name)

     17:34 

(Move log). .

[Yar‎ (3×)]

     

17:34

. . Yar (talk | contribs) moved page User talk:Yardena Cohen to User talk:Yar(Automatically moved page while renaming the user "Yardena Cohen" to "Yar")

     

17:34

. . Yar (talk | contribs) moved page User:Yardena Cohen/Notebook/attempt/Entry Base to User:Yar/Notebook/attempt/Entry Base(Automatically moved page while renaming the user "Yardena Cohen" to "Yar")

     

17:34

. . Yar (talk | contribs) moved page User:Yardena Cohen/Notebook/attempt to User:Yar/Notebook/attempt(Automatically moved page while renaming the user "Yardena Cohen" to "Yar")

     17:34 (User rename log) . . Yar (talk | contribs) renamed user Yardena Cohen (108 edits) to Yar(shorter)