BioMicroCenter:BorealAurora: Difference between revisions
(New page: {{BioMicroCenter}} == Boreal Aurora == ''' The Boreal is at MIT under a trial agreement. It will not remain at MIT unless there is sufficient demand for its services'''<BR> The Boreal Aur...) |
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[[Image:BMC_AuroraMachine.png|thumb|right|400xp|Boreal Aurora]] | |||
== Boreal Aurora == | == Boreal Aurora == | ||
''' The Boreal is at MIT under a trial agreement. It will not remain at MIT unless there is sufficient demand for its services'''<BR> | ''' The Boreal is at MIT under a trial agreement. It will not remain at MIT unless there is sufficient demand for its services'''<BR> | ||
The Boreal Aurora system is a DNA/RNA purification system designed to remove contaminants and concentrate DNA & RNA samples. The Aurora system claims 100x contamination reduction and up to 100x sample concentration. The Aurora takes 4h-1d to run and can process samples between 300bp and 1.1Mbp of DNA and 500bp to 1.1Mbp for RNA. | The Boreal Aurora system is a DNA/RNA purification system designed to remove contaminants and concentrate DNA & RNA samples. The Aurora system claims 100x contamination reduction and up to 100x sample concentration. The Aurora takes 4h-1d to run and can process samples between 300bp and 1.1Mbp of DNA and 500bp to 1.1Mbp for RNA. | ||
=== | ===POSSIBLE APPLICATIONS=== | ||
* Ultra-high-MW DNA from cell culture / agarose plugs | * Ultra-high-MW DNA from cell culture / agarose plugs | ||
* purification from low copy number bacterial samples | * purification from low copy number bacterial samples | ||
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===LIMITS=== | ===LIMITS=== | ||
Samples must be very low salt to be successful and within the size range of the machine (300bp-1.1Mbp). The machine can only handle one sample at a time so has quite limited throughput. | [[Image:BMC_AuroraCHIP.png|thumb|right|200px|SCODA Chip]] | ||
Samples must be very low salt to be successful and within the size range of the machine (300bp-1.1Mbp). Up to 5ml can be loaded. The machine can only handle one sample at a time so has quite limited throughput. | |||
Revision as of 08:28, 28 May 2013
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Boreal Aurora
The Boreal is at MIT under a trial agreement. It will not remain at MIT unless there is sufficient demand for its services
The Boreal Aurora system is a DNA/RNA purification system designed to remove contaminants and concentrate DNA & RNA samples. The Aurora system claims 100x contamination reduction and up to 100x sample concentration. The Aurora takes 4h-1d to run and can process samples between 300bp and 1.1Mbp of DNA and 500bp to 1.1Mbp for RNA.
POSSIBLE APPLICATIONS
- Ultra-high-MW DNA from cell culture / agarose plugs
- purification from low copy number bacterial samples
SCODA
The Boreal Aurora works using "synchronous coefficient of drag alteration" or SCODA. SCODA uses a linear electric field to remove contaminants and a rotating electric field to concentrate charged molecules in a centeral buffer well.
LIMITS
Samples must be very low salt to be successful and within the size range of the machine (300bp-1.1Mbp). Up to 5ml can be loaded. The machine can only handle one sample at a time so has quite limited throughput.
