Difference between revisions of "Beta-lactamase"

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(Plasmid stabilization)
 
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==Colorimetric reporter for B-lac activity==
 
==Colorimetric reporter for B-lac activity==
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==Plasmid stabilization==
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*Beta-lactamase gene ''bla'' or ''ampR'' is used in molecular cloning as a selection marker, since cells bearing plasmids encoding the gene are resistant to ampicillin and [[carbenicillin]].
  
 
NITROCEFIN (GLAXO RESEARCH 87/312)<br>
 
NITROCEFIN (GLAXO RESEARCH 87/312)<br>

Latest revision as of 10:28, 5 August 2009

Colorimetric reporter for B-lac activity

Plasmid stabilization

  • Beta-lactamase gene bla or ampR is used in molecular cloning as a selection marker, since cells bearing plasmids encoding the gene are resistant to ampicillin and carbenicillin.

NITROCEFIN (GLAXO RESEARCH 87/312)
Code: SR0112
For the rapid chromogenic detection of beta-lactamase activity.
Oxiod Inc. page on Nitrocefin

References

  1. ANDERSON ES and DATTA N. RESISTANCE TO PENICILLINS AND ITS TRANSFER IN ENTEROBACTERIACEAE. Lancet. 1965 Feb 20;1(7382):407-9. PubMed ID:14238093 | HubMed [Anderson-Lancet-1965]
  2. Datta N and Richmond MH. The purification and properties of a penicillinase whose synthesis is mediated by an R-factor in Escherichia coli. Biochem J. 1966 Jan;98(1):204-9. PubMed ID:5328167 | HubMed [Datta-BiochemJ-1966]
  3. Evans J, Galindo E, Olarte J, and Falkow S. Beta-lactamase of R factors. J Bacteriol. 1968 Oct;96(4):1441-2. PubMed ID:4971890 | HubMed [Evans-JBacteriol-1968]
  4. ISBN:0-87969-577-3 [MolecularCloning]
  5. Gao W, Xing B, Tsien RY, and Rao J. Novel fluorogenic substrates for imaging beta-lactamase gene expression. J Am Chem Soc. 2003 Sep 17;125(37):11146-7. DOI:10.1021/ja036126o | PubMed ID:16220906 | HubMed [Gao-JACS-2003]
All Medline abstracts: PubMed | HubMed

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