Difference between revisions of "Beta-glucuronidase protocols"

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(Materials)
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==Plate Screen==
 
==Plate Screen==
 
===Materials===
 
===Materials===
 +
*Media of choice
 +
*X-gluc stock solution (0.1M = 52.2mg/mL)
  
 
===Method===
 
===Method===

Revision as of 21:30, 4 July 2011


Introduction

These are methods to screen for and assay the activity of the common reporter enzyme β-Glucuronidase (GUS) activity. Because the catalytic activity of β-Glucuronidase is very similar to β-Galactosidase (LacZ) these protocols are also very similar to the LacZ protocols.

Plate Screen

Materials

  • Media of choice
  • X-gluc stock solution (0.1M = 52.2mg/mL)

Method

Notes

Assay

Materials

Method

Notes

Staining