Difference between revisions of "Beta-glucuronidase protocols"

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(New page: ==Introduction== These are methods to screen for and assay the activity of the common reporter enzyme β-Glucuronidase (GUS) activity. Because the catalytic activity of β-Glucuronidase...)
 
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Revision as of 21:21, 4 July 2011


Introduction

These are methods to screen for and assay the activity of the common reporter enzyme β-Glucuronidase (GUS) activity. Because the catalytic activity of β-Glucuronidase is very similar to β-Galactosidase (LacZ) these protocols are also very similar to the LacZ protocols.

Plate Screen

Materials

Method

Notes

Assay

Materials

Method

Notes

Staining