Difference between revisions of "Beta-galactosidase"
|Line 16:||Line 16:|
Revision as of 09:01, 26 July 2007
Some interesting facts 
- A tetramer of 4 identical subunits
- Each subunit is 120kD.
- Active only as a tetramer.
- Mutations in some of the codons of the N-terminal 60aa or C-terminal 100aa results in an inactive, dimeric β-galactosidase. 
- If the above sequences are deleted, the missing protein fragment can be replaced by the corresponding peptide. This is called intracistronic alpha or omega complementation respectively.
- Its N-terminal 23 residues can be replaced by any amino acid residues without affecting the enzymatic activity.
- A mutant with an internal deletion of codons 21-41 of the lacZ gene does not produce any active β-galactosidase.
- A mutant with a deletion of everything past residue 60 (i.e. it expresses only the first 60 N-terminal amino acids) does not produce any active β-galactosidase.