Berk2010-Gel Pictures: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
No edit summary
 
(5 intermediate revisions by the same user not shown)
Line 1: Line 1:
==[[User:Amy N. Kristofferson|Amy N. Kristofferson]] 17:49, 10 June 2010 (EDT)==
[[Image:Digestgel6-10-10.jpg]]
==[[User:Amy N. Kristofferson|Amy N. Kristofferson]] 19:16, 9 June 2010 (EDT)==
[[Image:6-9-10colonypcr.JPG | 1000px]] <br>
Lane 1,3,5,7: iGEM10_008a-d    Expected size: 1250 <br>
Lane 2,4,6,8: iGEM10_014a-d  Expected size: 1660  <br>
Lane 9,11,13,15: iGEM10_009a-d    Expected size: 480 <br>
Lane 10,12,14,16: iGEM10_015a-d  Expected size: 280 <br>
Lane 17,19,21,23,25,27,29,31: iGEM10_001a-h    Expected size: 1550 <br>
Lane 18,20,22,24: iGEM10_16a-d  Expected size: 1050
<br>
==[[User:Amy N. Kristofferson|Amy N. Kristofferson]] 17:56, 8 June 2010 (EDT)==
==[[User:Amy N. Kristofferson|Amy N. Kristofferson]] 17:56, 8 June 2010 (EDT)==
[[Image:6-8-10.jpg]]
[[Image:6-8-10.jpg]]


Colony PCR  
Colony PCR  
Lane 1 iGEM10_001a (should be 1500bp) <br>
Lane 1 iGEM10_003a (should be 1250bp) <br>
Lane 2 iGEM10_001b (should be 1500bp) <br>
Lane 2 iGEM10_003b (should be 1250bp) <br>
Lane 3 iGEM10_001c (should be 1500bp) <br>
Lane 3 iGEM10_003c (should be 1250bp) <br>
4 iGEM10_001d (should be 1500bp) <br>
4 iGEM10_003d (should be 1250bp) <br>
5 iGEM10_002a (should be 550bp) <br>
5 iGEM10_001a (should be 1500bp) <br>
6 iGEM10_002b (should be 550bp)<br>
6 iGEM10_001b (should be 1500bp)<br>
7 iGEM10_002c (should be 550bp)<br>
7 iGEM10_001c (should be 1500bp)<br>
8 iGEM10_002d (should be 550bp)<br>
8 iGEM10_001d (should be 1500bp)<br>
9 iGEM10_003a (should be 1250bp)<br>
9 iGEM10_002a (should be 550bp)<br>
10 iGEM10_003b (should be 1250bp)<br>
10 iGEM10_002b (should be 550bp)<br>
11 iGEM10_003c (should be 1250bp)<br>
11 iGEM10_002c (should be 550bp)<br>
12 iGEM10_003d (should be 1250bp)<br>
12 iGEM10_002d (should be 550bp)<br>


==[[User:Amy N. Kristofferson|Amy N. Kristofferson]] 16:55, 7 June 2010 (EDT)==
==[[User:Amy N. Kristofferson|Amy N. Kristofferson]] 16:55, 7 June 2010 (EDT)==

Latest revision as of 14:49, 10 June 2010

Amy N. Kristofferson 17:49, 10 June 2010 (EDT)

Amy N. Kristofferson 19:16, 9 June 2010 (EDT)


Lane 1,3,5,7: iGEM10_008a-d Expected size: 1250
Lane 2,4,6,8: iGEM10_014a-d Expected size: 1660
Lane 9,11,13,15: iGEM10_009a-d Expected size: 480
Lane 10,12,14,16: iGEM10_015a-d Expected size: 280
Lane 17,19,21,23,25,27,29,31: iGEM10_001a-h Expected size: 1550
Lane 18,20,22,24: iGEM10_16a-d Expected size: 1050


Amy N. Kristofferson 17:56, 8 June 2010 (EDT)

Colony PCR Lane 1 iGEM10_003a (should be 1250bp)
Lane 2 iGEM10_003b (should be 1250bp)
Lane 3 iGEM10_003c (should be 1250bp)
4 iGEM10_003d (should be 1250bp)
5 iGEM10_001a (should be 1500bp)
6 iGEM10_001b (should be 1500bp)
7 iGEM10_001c (should be 1500bp)
8 iGEM10_001d (should be 1500bp)
9 iGEM10_002a (should be 550bp)
10 iGEM10_002b (should be 550bp)
11 iGEM10_002c (should be 550bp)
12 iGEM10_002d (should be 550bp)

Amy N. Kristofferson 16:55, 7 June 2010 (EDT)

Colony PCR of 7+10 (we forgot to do this last week). Lane 1: ladder
Lane 2: sample a
Lane 3: b
Lane 4: c
Lane 5: d