Berk2006-StockBoxes

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Procedure: Combine equal volumes of cells and glycerol (50% cells, 50%glycerol). Freeze in cryo vial.

Box1

A B C D E F G H I
J01004 J01008 J01063 J01028 J01064 J01021 J01022 J01023 J01024(KAN
J01020 J01030 J01099 J01100 J01027 J01052 J01055 J01050 J01060
J01057 J01058 J01059 J01067 J01068 J01076 J01076 J01075 J01093
J01092 J01094 J01096 J01098 J01129/MC1061 J01002/MC1061 J01093/MC1061 pSB3C6-J01122/TGI pAC997-1/MC1061
J01001 J01002 J01003 J01109 J01021 J01022 J01023 J01124 J01126
J01127 J01066 pSB3C6-J01022-B BW20767 - pSB3C6-J01093 (TGI/Clone 1) pSB3C6-J01024 (TGI/Clone 1) TG1
pJ23006-I13522 pJ23006-J23007 pJ23006-J23008 pSB1A2-J23012 pSB1A2-J23018-2 "pSB1A2"-J01007 "pSB1A2"-J01006


Box2

A B C D E F G H I
LOCK2 ID500 IL2007 R0040 R0051 ROO80 pSB2K3 PK3 PK2 E0420 pSB1A2
E0430 E0840 13521 SB1A3 I3006 E0024 E1010 pSB2K3 C0051 C0040 tetR B0015
BBa_B0020 RBS strong B0034 E6030 pSB1Ak3 oriTF 0lambda oriTR oriTR lock1 traJR
oriTF on RFP J01020 J01021 TlambdaOlambda Rlambda T6 J01024 J01022 J01023
pSpoIIE Amp pSpoIIE kan pSB1A2-I13522 in JM109 pSB1A2-S03155 994 Library 06/27/06 pSB1A2-T9002 in TGI pSB1A2-P0412 in TGI pSB1A2-I13603 in TGI pSB1A2-F2620 in TGI
pSB1A2-I13601 in TGI pSB1AK3-F1610 in TGI pSB1A2-R0040+F1610#1_07/25/06 pSB1A2-F2620+P0412#1_07/25/06

Box3

A B C D E F G H I
pUC19 pKD46-1 pKD13 pKD46-2 RP4 (Amp,Kan,Tet) R35:tetR 2:1 RBS:tetR 2:1 RBS:cI RP1 x K1200 Kan
RP1 xK1200 ND RP4 (Amp,Kan,Tet) Top10 RP1 xK1200 Amp p0X38 Kan (F) PCV top10 Frost F R0040
B0015 E0430 pSB1A2 I05115 spo0A topo 1:1 spo0A topo 1:2 Terminator BBa_B0015 spoIIE topo 1:1 2:2 RBS:cI i51840
E0030 RBS strong R0040 pSB1A2 I0500 spoIIE topo 1:2 E0840 C0051 cI rep pSpoIIa + YFP I12007 pSB2K3 pKD46 in DH10B
pKD46 x Rlambda pKD46 x pOX38 pBca1020-Bca1022 lib hit C2 pBca1020-Bca1022 lib hit D7 pBca1020-Bca1022 lib hit C5 pBca1020-Bca1022 lib hit H12 pBca1020-Bca1022 lib hit E1 pBca1020-Bca1022 lib hit B10 pBca1020-Bca1022 lib hit E11
pBca1020-Bca1022 lib hit F3 pBca1020-Bca1022 lib hit A5 pBca1020-Bca1022 lib hit F6 pBca1020-Bca1022 lib hit E10 pBca1020-Bca1022 lib hit C1 pBca1020-Bca1022 lib hit B9 pBca1020-Bca1022 lib hit F4 pBca1020-Bca1022 lib hit D7 pBca1020-Bca1022 lib hit C2
pBca1020-Bca1022 lib hit F12 pBca1020-Bca1022 lib hit A2 pBca1020-Bca1022 lib hit E8 1022A 1022B 1022C 1022D 1022E

Box4: TlambdaOlambda F plasmid KCM competent cells.

Box5: Rlambda R plasmid KCM competent cells.

Box6: Glycerol Stocks of EC100D::pir+ and pOX38

Box7: pSB3C6-J01122 KCM Competent cells (orange and green stripes).

Box 8: TG1

Back Compartment: Olambda F plasmid (OriT knockout) KCM competent cells.