Difference between revisions of "Bacterial species identification"

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PMID 1987160
+
<cite>
 +
# pmid=1987160
  
PMID 2095137
+
# pmid=2095137
  
PMID 14967233
+
# pmid=14967233
  
PMID 14607398
+
# pmid=14607398
  
PMID 11240048
+
# pmid=11240048
  
PMID 1987160
+
# pmid=1987160
  
PMID 2095137
+
# pmid=2095137
  
PMID  2482222
+
# pmid=2482222
  
 +
</cite>
  
Mycoplasma universal primers
+
==Mycoplasma universal primers==
  
 
Johansson K-E, Characterization of mycoplasmas by PCR and sequence analysis iwth universal 16S rDNA primers, Chapter 18, Mycoplasma Protocols, 1998.
 
Johansson K-E, Characterization of mycoplasmas by PCR and sequence analysis iwth universal 16S rDNA primers, Chapter 18, Mycoplasma Protocols, 1998.
  
U1F  12-27  ttg atc ctg gct cag gay daa cg
+
* U1F  12-27  ttg atc ctg gct cag gay daa cg
 
+
* U2F  327-348  cca rac tcc tac ggr agg c
U2F  327-348  cca rac tcc tac ggr agg c
+
* U3R  512-527  att acc gcg gck gct g
 
+
* U4F  792-810  gta gtc cac gcc gta aac g
U3R  512-527  att acc gcg gck gct g
+
* U5R  902-924  ctt gtg cgg gyy ccc gtc aa
 
+
* U6F  1154-1172  gag gaa ggy grg gay gay g
U4F  792-810  gta gtc cac gcc gta aac g
+
* U7R  1359-1374  aca agr ccc gag aac g
 
+
* U8R  1502-1524  gaa agg agg trw tcc ayc csc ac
U5R  902-924  ctt gtg cgg gyy ccc gtc aa
 
 
 
U6F  1154-1172  gag gaa ggy grg gay gay g
 
 
 
U7R  1359-1374  aca agr ccc gag aac g
 
 
 
U8R  1502-1524  gaa agg agg trw tcc ayc csc ac
 
  
  
 
Lane, D. J. (1991). 16S/23S rRNA sequencing. Nucleic acid techniques in bacterial systematics. E. Stackebrandt and M. Goodfellow, eds. New York, NY, John Wiley and Sons: 115-175.
 
Lane, D. J. (1991). 16S/23S rRNA sequencing. Nucleic acid techniques in bacterial systematics. E. Stackebrandt and M. Goodfellow, eds. New York, NY, John Wiley and Sons: 115-175.
  
27f 5'AGAGTTTGATCMTGGCTCAG
+
* 27f 5'AGAGTTTGATCMTGGCTCAG
342r 5'CTGCTGCSYCCCGTAG
+
* 342r 5'CTGCTGCSYCCCGTAG
357f 5'CTCCTACGGGAGGCAGCAG
+
* 357f 5'CTCCTACGGGAGGCAGCAG
519r 5'GWATTACCGCGGCKGCTG
+
* 519r 5'GWATTACCGCGGCKGCTG
530f 5'GTGCCAGCMGCCGCGG
+
* 530f 5'GTGCCAGCMGCCGCGG
1100r 5'GGGTTGCGCTCGTTG
+
* 1100r 5'GGGTTGCGCTCGTTG
1114f 5'GCAACGAGCGCAACCC
+
* 1114f 5'GCAACGAGCGCAACCC
1392r 5'ACGGGCGGTGTGTRC
+
* 1392r 5'ACGGGCGGTGTGTRC
1406f 5'TGYACACACCTCCCGT
+
* 1406f 5'TGYACACACCTCCCGT
1492r 5'TACGGYTACCTTGTTACGACTT
+
* 1492r 5'TACGGYTACCTTGTTACGACTT
1525r 5'AAGGAGGTGWTCCARCC
+
* 1525r 5'AAGGAGGTGWTCCARCC

Revision as of 18:50, 11 April 2006

[1, 1, 1, 1, 1, 1, 1, 1, 2, 2, 3, 3, 4, 5, 6, 7]

Mycoplasma universal primers

Johansson K-E, Characterization of mycoplasmas by PCR and sequence analysis iwth universal 16S rDNA primers, Chapter 18, Mycoplasma Protocols, 1998.

  • U1F 12-27 ttg atc ctg gct cag gay daa cg
  • U2F 327-348 cca rac tcc tac ggr agg c
  • U3R 512-527 att acc gcg gck gct g
  • U4F 792-810 gta gtc cac gcc gta aac g
  • U5R 902-924 ctt gtg cgg gyy ccc gtc aa
  • U6F 1154-1172 gag gaa ggy grg gay gay g
  • U7R 1359-1374 aca agr ccc gag aac g
  • U8R 1502-1524 gaa agg agg trw tcc ayc csc ac


Lane, D. J. (1991). 16S/23S rRNA sequencing. Nucleic acid techniques in bacterial systematics. E. Stackebrandt and M. Goodfellow, eds. New York, NY, John Wiley and Sons: 115-175.

  • 27f 5'AGAGTTTGATCMTGGCTCAG
  • 342r 5'CTGCTGCSYCCCGTAG
  • 357f 5'CTCCTACGGGAGGCAGCAG
  • 519r 5'GWATTACCGCGGCKGCTG
  • 530f 5'GTGCCAGCMGCCGCGG
  • 1100r 5'GGGTTGCGCTCGTTG
  • 1114f 5'GCAACGAGCGCAACCC
  • 1392r 5'ACGGGCGGTGTGTRC
  • 1406f 5'TGYACACACCTCCCGT
  • 1492r 5'TACGGYTACCTTGTTACGACTT
  • 1525r 5'AAGGAGGTGWTCCARCC