Difference between revisions of "BYU iGEM/Notebook/16 February 2011"

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#Check PCR
 
#Check PCR
*if good, restriction enzyme digest
+
#*if good, restriction enzyme digest
*if not good, PCR again
+
#*if not good, PCR again
 
#Plasmid:
 
#Plasmid:
 
*extract from E. coli
 
*extract from E. coli

Revision as of 15:37, 16 February 2011

OxySoxy Team To Do:

  1. Check PCR
    • if good, restriction enzyme digest
    • if not good, PCR again
  2. Plasmid:
  • extract from E. coli
  • PCR amplification

ΔTS Team To Do:

  1. PCR amplification of primers
  2. Plasmid extraction
  3. Insertion