Difference between revisions of "BYU iGEM/Notebook/16 February 2011"

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==OxySoxy Team To Do:==
 
==OxySoxy Team To Do:==
  
Check PCR
+
#Check PCR
 
*if good, restriction enzyme digest
 
*if good, restriction enzyme digest
 
*if not good, PCR again
 
*if not good, PCR again
  
Plasmid:
+
##Plasmid:
 
*extract from E. coli
 
*extract from E. coli
 
*PCR amplification
 
*PCR amplification

Revision as of 15:36, 16 February 2011

OxySoxy Team To Do:

  1. Check PCR
  • if good, restriction enzyme digest
  • if not good, PCR again
    1. Plasmid:
  • extract from E. coli
  • PCR amplification

ΔTS Team To Do:

  1. PCR amplification of primers
  2. Plasmid extraction
  3. Insertion