Difference between revisions of "BYU iGEM/Notebook/16 February 2011"

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(New page: ==OxySoxy Team To Do:== Check PCR if good, restriction enzyme digest if not good, PCR again Plasmid: *extract from E. coli *PCR amplification ==ΔTS Team To Do:== PCR amplification of ...)
 
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Check PCR
 
Check PCR
if good, restriction enzyme digest
+
*if good, restriction enzyme digest
if not good, PCR again
+
*if not good, PCR again
  
 
Plasmid:
 
Plasmid:
Line 11: Line 11:
 
==ΔTS Team To Do:==
 
==ΔTS Team To Do:==
  
PCR amplification of primers
+
#PCR amplification of primers
Plasmid extraction
+
#Plasmid extraction
Insertion
+
#Insertion

Revision as of 15:36, 16 February 2011

OxySoxy Team To Do:

Check PCR

  • if good, restriction enzyme digest
  • if not good, PCR again

Plasmid:

  • extract from E. coli
  • PCR amplification

ΔTS Team To Do:

  1. PCR amplification of primers
  2. Plasmid extraction
  3. Insertion