Difference between revisions of "BME103 s2013:TEMPwu2"

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(Data Analysis)
 
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=LAB 2 WRITE-UP=
 
=LAB 2 WRITE-UP=
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'''SYBR Green Dye'''<br>
 
'''SYBR Green Dye'''<br>
''A short summary describing SYBR green and how it is used to detect double-stranded DNA''<br>
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''[A short summary describing SYBR green dye]''<br>
  
  
 
'''Single-Drop Fluorimeter'''<br>
 
'''Single-Drop Fluorimeter'''<br>
''A PHOTO and description of the single-drop fluorimeter''<br>
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''[A description of the single-drop fluorimeter device. Add a PHOTO for bonus points]''<br>
  
  
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'''How the Fluorescence Technique Works'''<br>
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''[In your own words, a summary of the information from page 9 of the worksheet]''
  
<br><br>
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<br>
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<!-- Note: Be sure to delete the text in brackets: ''[ ]'' -->
  
 
==Procedure==
 
==Procedure==
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'''Calibration'''<br>
 
'''Calibration'''<br>
  
''[Place a PHOTO that shows your camera phone set up in front of the flourimeter here]''
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''[Describe how to set up your camera in front of the fluorimeter. Add a PHOTO of this set-up for bonus points.]''
  
 
* Distance between the smart phone cradle and drop =
 
* Distance between the smart phone cradle and drop =
<br>''[See worksheet page 5]''
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''[See worksheet page 5]''
  
  
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| row 2 cell 1 || row 2 cell 2 || row 2 cell 3 || row 2 cell 4
 
| row 2 cell 1 || row 2 cell 2 || row 2 cell 3 || row 2 cell 4
 
|-
 
|-
| row 2 cell 1 || row 2 cell 2 || row 2 cell 3 || row 2 cell 4
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| row 3 cell 1 || row 3 cell 2 || row 3 cell 3 || row 3 cell 4
 
|}
 
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''[Add more rows as needed]''
  
  
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'''Placing Samples onto the Fluorimeter'''
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* ''[Step one, in your OWN words]''
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* ''[Step two, in your own words]''
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* ''[Step three, in your own words]''
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* ''[etc.]''
  
<br><br>
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<br>
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==Data Analysis==
 
==Data Analysis==
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| row 3 cell 1 || row 3 cell 2 || row 3 cell 3 || row 3 cell 4 || row 2 cell 5
 
| row 3 cell 1 || row 3 cell 2 || row 3 cell 3 || row 3 cell 4 || row 2 cell 5
 
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''[Add more rows as needed]''
 
''[Add more rows as needed]''
  
  
'''Fitting a Straight Line'''
 
  
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'''Fitting a Straight Line'''<br>
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''[Place an IMAGE of your Excel plot with a line of best fit here. See worksheet page 8]''
  
  
<br><br>
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<br>
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Latest revision as of 15:05, 26 March 2013

Owwnotebook icon.png BME 103 Spring 2013 Home
People
Lab Write-Up 1
Lab Write-Up 2
Lab Write-Up 3
Course Logistics For Instructors
Photos
Wiki Editing Help
BME494 Asu logo.png


OUR TEAM

Name: student
Name: student
Name: student
Name: student
Name: student
Name: student


LAB 2 WRITE-UP

Background Information

SYBR Green Dye
[A short summary describing SYBR green dye]


Single-Drop Fluorimeter
[A description of the single-drop fluorimeter device. Add a PHOTO for bonus points]


How the Fluorescence Technique Works
[In your own words, a summary of the information from page 9 of the worksheet]



Procedure

Smart Phone Camera Settings

  • [The type of smart phone you used and how you adjusted the camera settings, if applicable (see worksheet page 4).]
    • Flash:
    • ISO setting:
    • White Balance:
    • Exposure:
    • Saturation:
    • Contrast:
  • [If you used an additional phone, describe the other type of smart phone you used and how you adjusted the camera settings, if applicable (see worksheet page 4).]
    • Flash:
    • ISO setting:
    • White Balance:
    • Exposure:
    • Saturation:
    • Contrast:


Calibration

[Describe how to set up your camera in front of the fluorimeter. Add a PHOTO of this set-up for bonus points.]

  • Distance between the smart phone cradle and drop =

[See worksheet page 5]


Solutions Used for Calibration [See worksheet page 5]

row 1 cell 1 row 1 cell 2 row 1 cell 3 row 1 cell 4
row 2 cell 1 row 2 cell 2 row 2 cell 3 row 2 cell 4
row 3 cell 1 row 3 cell 2 row 3 cell 3 row 3 cell 4

[Add more rows as needed]


Placing Samples onto the Fluorimeter

  • [Step one, in your OWN words]
  • [Step two, in your own words]
  • [Step three, in your own words]
  • [etc.]


Data Analysis

Representative Images of Samples

[Show an IMAGE where you drew a circle around the droplet with the freehand tool for a sample with no DNA]

[Show an IMAGE where you drew a circle around the droplet with the freehand tool for a sample with DNA (positive signal)]


Image J Values for All Samples [See worksheet page 5]

row 1 cell 1 row 1 cell 2 row 1 cell 3 row 1 cell 4 row 1 cell 5
row 2 cell 1 row 2 cell 2 row 2 cell 3 row 2 cell 4 row 2 cell 5
row 3 cell 1 row 3 cell 2 row 3 cell 3 row 3 cell 4 row 2 cell 5

[Add more rows as needed]


Fitting a Straight Line
[Place an IMAGE of your Excel plot with a line of best fit here. See worksheet page 8]