Difference between revisions of "BME103 s2013:TEMPwu2"

From OpenWetWare
Jump to: navigation, search
(Protocols)
 
(14 intermediate revisions by the same user not shown)
Line 21: Line 21:
 
| [[Image:BME103student.jpg|100px|thumb|Name: student]]
 
| [[Image:BME103student.jpg|100px|thumb|Name: student]]
 
|}
 
|}
 +
 +
<!-- Note: Delete any image placeholders that you do not need. -->
  
 
=LAB 2 WRITE-UP=
 
=LAB 2 WRITE-UP=
Line 28: Line 30:
  
 
'''SYBR Green Dye'''<br>
 
'''SYBR Green Dye'''<br>
''A short summary describing SYBR green and how it is used to detect double-stranded DNA''<br>
+
''[A short summary describing SYBR green dye]''<br>
  
  
 
'''Single-Drop Fluorimeter'''<br>
 
'''Single-Drop Fluorimeter'''<br>
''A PHOTO and description of the single-drop fluorimeter''<br>
+
''[A description of the single-drop fluorimeter device. Add a PHOTO for bonus points]''<br>
  
  
 +
'''How the Fluorescence Technique Works'''<br>
 +
''[In your own words, a summary of the information from page 9 of the worksheet]''
  
<br><br>
+
 
 +
<br>
 +
<!-- Note: Be sure to delete the text in brackets: ''[ ]'' -->
  
 
==Procedure==
 
==Procedure==
  
 
'''Smart Phone Camera Settings'''<br>
 
'''Smart Phone Camera Settings'''<br>
''The type of smart phone you used and how you adjusted the camera settings, if applicable (see worksheet page 4).''<br>
+
* ''[The type of smart phone you used and how you adjusted the camera settings, if applicable (see worksheet page 4).]''
 +
** Flash:
 +
** ISO setting:
 +
** White Balance:
 +
** Exposure:
 +
** Saturation:
 +
** Contrast:
 +
 
 +
* ''[If you used an additional phone, describe the other type of smart phone you used and how you adjusted the camera settings, if applicable (see worksheet page 4).]''
 +
** Flash:
 +
** ISO setting:
 +
** White Balance:
 +
** Exposure:
 +
** Saturation:
 +
** Contrast:
  
  
 
'''Calibration'''<br>
 
'''Calibration'''<br>
  
''[Place a PHOTO of your camera phone set up in front of the flourimeter here]''
+
''[Describe how to set up your camera in front of the fluorimeter. Add a PHOTO of this set-up for bonus points.]''
  
* Distance between your smart phone cradle and drop =
+
* Distance between the smart phone cradle and drop =
 +
''[See worksheet page 5]''
  
{| {{table}}
+
 
 +
'''Solutions Used for Calibration''' ''[See worksheet page 5]''
 +
{| {{table}} width=700
 
|-
 
|-
 
| row 1 cell 1 || row 1 cell 2 || row 1 cell 3 || row 1 cell 4
 
| row 1 cell 1 || row 1 cell 2 || row 1 cell 3 || row 1 cell 4
 
|-
 
|-
 
| row 2 cell 1 || row 2 cell 2 || row 2 cell 3 || row 2 cell 4
 
| row 2 cell 1 || row 2 cell 2 || row 2 cell 3 || row 2 cell 4
 +
|-
 +
| row 3 cell 1 || row 3 cell 2 || row 3 cell 3 || row 3 cell 4
 
|}
 
|}
 +
''[Add more rows as needed]''
  
  
'''DNA Sample Set-up Procedure'''
+
'''Placing Samples onto the Fluorimeter'''
# Step 1
+
* ''[Step one, in your OWN words]''
# Step 2
+
* ''[Step two, in your own words]''
# Step 3...
+
* ''[Step three, in your own words]''
 +
* ''[etc.]''
  
 +
<br>
 +
<!-- Note: Be sure to delete the text in brackets: ''[ ]'' -->
  
'''PCR Reaction Mix'''
+
==Data Analysis==
* What is in the PCR reaction mix?
 
  
 +
'''Representative Images of Samples'''
  
'''DNA/ primer mix'''
+
''[Show an IMAGE <u>where you drew a circle around the droplet</u> with the freehand tool for a sample with no DNA]''
* What is in the DNA/ primer mix?
 
  
 +
''[Show an IMAGE <u>where you drew a circle around the droplet</u> with the freehand tool for a sample '''with''' DNA (positive signal)]''
  
  
  
<br><br>
+
'''Image J Values for All Samples''' ''[See worksheet page 5]''
 
+
{| {{table}} width=700
==Research and Development==
+
|-
 +
| row 1 cell 1 || row 1 cell 2 || row 1 cell 3 || row 1 cell 4 || row 1 cell 5
 +
|-
 +
| row 2 cell 1 || row 2 cell 2 || row 2 cell 3 || row 2 cell 4 || row 2 cell 5
 +
|-
 +
| row 3 cell 1 || row 3 cell 2 || row 3 cell 3 || row 3 cell 4 || row 2 cell 5
 +
|}
 +
''[Add more rows as needed]''
  
'''Specific Cancer Marker Detection - The Underlying Technology'''<br>
 
  
(Add a write-up of the information discussed in Week 3's class)<br>
 
  
(BONUS points: Use a program like Powerpoint, Word, Illustrator, Microsoft Paint, etc. to illustrate how primers bind to the cancer DNA template, and how Taq polymerases amplify the DNA. Screen-captures from the OpenPCR tutorial might be useful. Be sure to credit the source if you borrow images.)
+
'''Fitting a Straight Line'''<br>
 +
''[Place an IMAGE of your Excel plot with a line of best fit here. See worksheet page 8]''
  
  
<br><br>
+
<br>
 +
<!-- Note: Be sure to delete the text in brackets: ''[ ]'' -->
  
  

Latest revision as of 15:05, 26 March 2013

Owwnotebook icon.png BME 103 Spring 2013 Home
People
Lab Write-Up 1
Lab Write-Up 2
Lab Write-Up 3
Course Logistics For Instructors
Photos
Wiki Editing Help
BME494 Asu logo.png


OUR TEAM

Name: student
Name: student
Name: student
Name: student
Name: student
Name: student


LAB 2 WRITE-UP

Background Information

SYBR Green Dye
[A short summary describing SYBR green dye]


Single-Drop Fluorimeter
[A description of the single-drop fluorimeter device. Add a PHOTO for bonus points]


How the Fluorescence Technique Works
[In your own words, a summary of the information from page 9 of the worksheet]



Procedure

Smart Phone Camera Settings

  • [The type of smart phone you used and how you adjusted the camera settings, if applicable (see worksheet page 4).]
    • Flash:
    • ISO setting:
    • White Balance:
    • Exposure:
    • Saturation:
    • Contrast:
  • [If you used an additional phone, describe the other type of smart phone you used and how you adjusted the camera settings, if applicable (see worksheet page 4).]
    • Flash:
    • ISO setting:
    • White Balance:
    • Exposure:
    • Saturation:
    • Contrast:


Calibration

[Describe how to set up your camera in front of the fluorimeter. Add a PHOTO of this set-up for bonus points.]

  • Distance between the smart phone cradle and drop =

[See worksheet page 5]


Solutions Used for Calibration [See worksheet page 5]

row 1 cell 1 row 1 cell 2 row 1 cell 3 row 1 cell 4
row 2 cell 1 row 2 cell 2 row 2 cell 3 row 2 cell 4
row 3 cell 1 row 3 cell 2 row 3 cell 3 row 3 cell 4

[Add more rows as needed]


Placing Samples onto the Fluorimeter

  • [Step one, in your OWN words]
  • [Step two, in your own words]
  • [Step three, in your own words]
  • [etc.]


Data Analysis

Representative Images of Samples

[Show an IMAGE where you drew a circle around the droplet with the freehand tool for a sample with no DNA]

[Show an IMAGE where you drew a circle around the droplet with the freehand tool for a sample with DNA (positive signal)]


Image J Values for All Samples [See worksheet page 5]

row 1 cell 1 row 1 cell 2 row 1 cell 3 row 1 cell 4 row 1 cell 5
row 2 cell 1 row 2 cell 2 row 2 cell 3 row 2 cell 4 row 2 cell 5
row 3 cell 1 row 3 cell 2 row 3 cell 3 row 3 cell 4 row 2 cell 5

[Add more rows as needed]


Fitting a Straight Line
[Place an IMAGE of your Excel plot with a line of best fit here. See worksheet page 8]