Difference between revisions of "BME103 s2013:TEMPwu2"

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(Procedure)
(Procedure)
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'''Calibration'''<br>
 
'''Calibration'''<br>
  
''[Place a PHOTO of your camera phone set up in front of the flourimeter here]''
+
''[Place a PHOTO that shows your camera phone set up in front of the flourimeter here]''
  
 
* Distance between the smart phone cradle and drop =
 
* Distance between the smart phone cradle and drop =
Line 75: Line 75:
 
| row 2 cell 1 || row 2 cell 2 || row 2 cell 3 || row 2 cell 4
 
| row 2 cell 1 || row 2 cell 2 || row 2 cell 3 || row 2 cell 4
 
|}
 
|}
 
 
'''DNA Sample Set-up Procedure'''
 
# Step 1
 
# Step 2
 
# Step 3...
 
 
 
'''PCR Reaction Mix'''
 
* What is in the PCR reaction mix?
 
 
 
'''DNA/ primer mix'''
 
* What is in the DNA/ primer mix?
 
 
  
  

Revision as of 00:39, 26 March 2013

Owwnotebook icon.png BME 103 Spring 2013 Home
People
Lab Write-Up 1
Lab Write-Up 2
Lab Write-Up 3
Course Logistics For Instructors
Photos
Wiki Editing Help
BME494 Asu logo.png


OUR TEAM

Name: student
Name: student
Name: student
Name: student
Name: student
Name: student

LAB 2 WRITE-UP

Background Information

SYBR Green Dye
A short summary describing SYBR green and how it is used to detect double-stranded DNA


Single-Drop Fluorimeter
A PHOTO and description of the single-drop fluorimeter




Procedure

Smart Phone Camera Settings

  • [The type of smart phone you used and how you adjusted the camera settings, if applicable (see worksheet page 4).]
    • Flash:
    • ISO setting:
    • White Balance:
    • Exposure:
    • Saturation:
    • Contrast:
  • [If you used an additional phone, describe the other type of smart phone you used and how you adjusted the camera settings, if applicable (see worksheet page 4).]
    • Flash:
    • ISO setting:
    • White Balance:
    • Exposure:
    • Saturation:
    • Contrast:


Calibration

[Place a PHOTO that shows your camera phone set up in front of the flourimeter here]

  • Distance between the smart phone cradle and drop =


[See worksheet page 5]


Solutions Used for Calibration [See worksheet page 5]

row 1 cell 1 row 1 cell 2 row 1 cell 3 row 1 cell 4
row 2 cell 1 row 2 cell 2 row 2 cell 3 row 2 cell 4
row 2 cell 1 row 2 cell 2 row 2 cell 3 row 2 cell 4




Research and Development

Specific Cancer Marker Detection - The Underlying Technology

(Add a write-up of the information discussed in Week 3's class)

(BONUS points: Use a program like Powerpoint, Word, Illustrator, Microsoft Paint, etc. to illustrate how primers bind to the cancer DNA template, and how Taq polymerases amplify the DNA. Screen-captures from the OpenPCR tutorial might be useful. Be sure to credit the source if you borrow images.)