Difference between revisions of "BME103 s2013:TEMPwu2"

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(OUR TEAM)
(Initial Machine Testing)
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=LAB 2 WRITE-UP=
 
=LAB 2 WRITE-UP=
  
==Initial Machine Testing==
+
==Background Information==
  
  
'''The Original Design'''<br>
+
'''SYBR Green Dye'''<br>
(Add image of the full OpenPCR machine here, from the Week 9 exercise. Write a paragraph description for visitors who have no idea what this is)<br>
+
''A short summary describing SYBR green and how it is used to detect double-stranded DNA''<br>
  
  
'''Experimenting With the Connections'''<br>
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'''Single-Drop Fluorimeter'''<br>
 
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''A PHOTO and description of the single-drop fluorimeter''<br>
When we unplugged (part 3) from (part 6), the machine ... (did what? fill in your answer)
 
 
 
When we unplugged the white wire that connects (part 6) to (part 2), the machine ... (did what? fill in your answer)
 
 
 
 
 
'''Test Run'''
 
 
 
(Write the date you first tested Open PCR and your experience(s) with the machine)<br>
 
  
  

Revision as of 00:20, 26 March 2013

Owwnotebook icon.png BME 103 Spring 2013 Home
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Lab Write-Up 1
Lab Write-Up 2
Lab Write-Up 3
Course Logistics For Instructors
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OUR TEAM

Name: student
Name: student
Name: student
Name: student
Name: student
Name: student

LAB 2 WRITE-UP

Background Information

SYBR Green Dye
A short summary describing SYBR green and how it is used to detect double-stranded DNA


Single-Drop Fluorimeter
A PHOTO and description of the single-drop fluorimeter




Protocols

Thermal Cycler Program


DNA Sample Set-up

row 1 cell 1 row 1 cell 2 row 1 cell 3 row 1 cell 4
row 2 cell 1 row 2 cell 2 row 2 cell 3 row 2 cell 4


DNA Sample Set-up Procedure

  1. Step 1
  2. Step 2
  3. Step 3...


PCR Reaction Mix

  • What is in the PCR reaction mix?


DNA/ primer mix

  • What is in the DNA/ primer mix?





Research and Development

Specific Cancer Marker Detection - The Underlying Technology

(Add a write-up of the information discussed in Week 3's class)

(BONUS points: Use a program like Powerpoint, Word, Illustrator, Microsoft Paint, etc. to illustrate how primers bind to the cancer DNA template, and how Taq polymerases amplify the DNA. Screen-captures from the OpenPCR tutorial might be useful. Be sure to credit the source if you borrow images.)