Difference between revisions of "BME103 s2013:T900 Group6 L2"

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(Procedure)
(Procedure)
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'''Smart Phone Camera Settings'''<br>
 
'''Smart Phone Camera Settings'''<br>
* ''[The type of smart phone you used and how you adjusted the camera settings, if applicable (see worksheet page 4).]''
+
* ''Iphone 5''
** Flash:
+
** Flash: None
** ISO setting:
+
** ISO setting: NA
** White Balance:  
+
** White Balance: NA
** Exposure:
+
** Exposure: NA
** Saturation:
+
** Saturation: NA
** Contrast:
+
** Contrast: NA
  
* ''[If you used an additional phone, describe the other type of smart phone you used and how you adjusted the camera settings, if applicable (see worksheet page 4).]''
+
'''Calibration'''<br>
** Flash:
+
 
** ISO setting:
+
Photo of Fluorimeter
** White Balance:
 
** Exposure:
 
** Saturation:
 
** Contrast:
 
  
  
'''Calibration'''<br>
 
  
''[Place a PHOTO that shows your camera phone set up in front of the flourimeter here]''
 
  
* Distance between the smart phone cradle and drop =
+
* Distance between the smart phone cradle and drop = 8cm
''[See worksheet page 5]''
 
  
  
'''Solutions Used for Calibration''' ''[See worksheet page 5]''
+
'''Solutions Used for Calibration'''  
 
{| {{table}} width=700
 
{| {{table}} width=700
 
|-
 
|-
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'''Placing Samples onto the Fluorimeter'''
 
'''Placing Samples onto the Fluorimeter'''
* ''[Step one, in your OWN words]''
+
* Get Slide, Micropipette and vial of sample
* ''[Step two, in your own words]''
+
* Place slide into fluorimeter
* ''[Step three, in your own words]''
+
* Take 80 micro liters of sample into micropipette
* ''[etc.]''
+
* Place 4 drops onto the slide, slide the slide over to where the light hits, don't use the same place twice
 +
* Make sure the drops form a ball and don't spread around individually
  
 
<br>
 
<br>

Revision as of 09:52, 26 March 2013

Owwnotebook icon.png BME 103 Spring 2013 Home
People
Lab Write-Up 1
Lab Write-Up 2
Lab Write-Up 3
Course Logistics For Instructors
Photos
Wiki Editing Help
BME494 Asu logo.png


OUR TEAM

Name: student
Name: student
Name: student
Name: student
Name: student
Name: student


LAB 2 WRITE-UP

Background Information

SYBR Green Dye
[A short summary describing SYBR green dye]


Single-Drop Fluorimeter
[A PHOTO and description of the single-drop fluorimeter device]


How the Fluorescence Technique Works
[In your own words, a summary of the information from page 9 of the worksheet]



Procedure

Smart Phone Camera Settings

  • Iphone 5
    • Flash: None
    • ISO setting: NA
    • White Balance: NA
    • Exposure: NA
    • Saturation: NA
    • Contrast: NA

Calibration

Photo of Fluorimeter



  • Distance between the smart phone cradle and drop = 8cm


Solutions Used for Calibration

Calf Thymus DNA solution concentration (microg/mL) Volume of the 2X DNA solution (uL) Volume of the SYBR GREEN I Dye Solution (uL) Final DNA concentration in PicoGreen Assay (ng/mL)
0 80 80 blank
.25 80 80 .125
.5 80 80 .25
1 80 80 .5
2 80 80 1
5 80 80 2.5


Placing Samples onto the Fluorimeter

  • Get Slide, Micropipette and vial of sample
  • Place slide into fluorimeter
  • Take 80 micro liters of sample into micropipette
  • Place 4 drops onto the slide, slide the slide over to where the light hits, don't use the same place twice
  • Make sure the drops form a ball and don't spread around individually


Data Analysis

Representative Images of Samples

[Show an IMAGE where you drew a circle around the droplet with the freehand tool for a sample with no DNA]

[Show an IMAGE where you drew a circle around the droplet with the freehand tool for a sample with DNA (positive signal)]


Image J Values for All Samples [See worksheet page 5]

row 1 cell 1 row 1 cell 2 row 1 cell 3 row 1 cell 4 row 1 cell 5
row 2 cell 1 row 2 cell 2 row 2 cell 3 row 2 cell 4 row 2 cell 5
row 3 cell 1 row 3 cell 2 row 3 cell 3 row 3 cell 4 row 2 cell 5

[Add more rows as needed]


Fitting a Straight Line
[Place an IMAGE of your Excel plot with a line of best fit here. See worksheet page 8]