BME103 s2013:T900 Group6

BME 103 Fall 2012	Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help
OUR TEAM Name: Cyril Wassef -Initial Machine Tester Name: Israel Brewer Research and Development Name: student Role(s) Name: student Role(s) Name: student Role(s) Name: student Role(s) LAB 1 WRITE-UP Initial Machine Testing The Original Design Description: This is a PCR machine. It is a piece of equipment that allows scientists to create different amounts of DNA sequences. With it, scientists are able to denature (break down) specific DNA strands by inducing heat, and thus are able to analyze them specifically. This machine helps with research on possible DNA mutation, and aids in on-going research to help resolve today's problems with diseases such as cancer. Experimenting With the Connections When we unplugged the LCD screen from the OpenPCR circuit board, the machine's LED failed to work properly. When we unplugged the white wire that connects the OpenPCR circuit board to the main heating block, the machine began to show a change of the temperature on the LCD screen. Test Run The date the PCR machine was open to conduct an analysis was on March 5th, 2013. We had machine number 6 that day. What we came to see, in terms of pros and cons, contained the following: Pros: - Lightweight - Quiet - Ease of Use Cons: - Took a while to complete the full cycle - The lid is not easy to remove. Requires some work - Requires a computer - Small and somewhat heavy - Made of Wood (fire hazard) Dale Franco L. Caagbay:==Protocols== Thermal Cycler Program DNA Sample Set-up Positive Control - Cancer Patient 1- Vial 1 Patient 1- Vial 2 Patient 1- Vial 3 Negative Control - No Cancer Patient 2- Vial 1 Patient 2- Vial 2 Patient 2- Vial 3 DNA Sample Set-up Procedure 1. Obtain DNA samples, PCR, DNA/Primer vials 2. Open All The Tops 3. Use pipette to move DNA into vials containing PCR mix PCR Reaction Mix What is in the PCR reaction mix? The PCR mix is made up of 50 Microliters of PCR. DNA/ primer mix What is in the DNA/ primer mix? The DNA/Primer Mix is made of 50 micro liters of DNA of patients 1 and 2, and primer. There are 2 vials containing negative and positive control. The negative control does not have cancer DNA, and the positive control has cancer in it. Research and Development Specific Cancer Marker Detection - The Underlying Technology (Add a write-up of the information discussed in Week 3's class) (BONUS points: Use a program like Powerpoint, Word, Illustrator, Microsoft Paint, etc. to illustrate how primers bind to the cancer DNA template, and how Taq polymerases amplify the DNA. Screen-captures from the OpenPCR tutorial might be useful. Be sure to credit the source if you borrow images.)