BME103 s2013:T900 Group6: Difference between revisions
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| [[Image:cyrilwassef.jpg|100px|thumb|Name: Cyril Wassef<br>-Initial Machine Tester]] | | [[Image:cyrilwassef.jpg|100px|thumb|Name: Cyril Wassef<br>-Initial Machine Tester]] | ||
| [[Image: | | [[Image:batman.jpg|100px|thumb|Name: Israel Brewer<br>Research and Development]] | ||
| [[Image:BME103student.jpg|100px|thumb|Name: student<br>Role(s)]] | | [[Image:BME103student.jpg|100px|thumb|Name: student<br>Role(s)]] | ||
| [[Image:BME103student.jpg|100px|thumb|Name: student<br>Role(s)]] | | [[Image:BME103student.jpg|100px|thumb|Name: student<br>Role(s)]] |
Revision as of 23:21, 25 March 2013
BME 103 Fall 2012 | Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help | ||||||||||||||
OUR TEAMLAB 1 WRITE-UPInitial Machine TestingThe Original Design
When we unplugged the LCD screen from the OpenPCR circuit board, the machine's LED failed to work properly. When we unplugged the white wire that connects the OpenPCR circuit board to the main heating block, the machine began to show a change of the temperature on the LCD screen.
The date the PCR machine was open to conduct an analysis was on March 5th, 2013. We had machine number 6 that day. What we came to saw, in terms of pros and cons, contained the following: Pros: - Lightweight - Quiet - Ease of Use Cons:
- Took a while to complete the full cycle
- The lid is not easy to remove. Requires some work
- Requires a computer
- Small and somewhat heavy
- Made of Wood (fire hazard)
ProtocolsThermal Cycler Program DNA Sample Set-up
DNA Sample Set-up Procedure 1. Obtain DNA samples, PCR, DNA/Primer vials 2. Open All The Tops 3. Use pipette to move DNA into vials containing PCR mix PCR Reaction Mix
The PCR mix is made up of 50 Microliters of PCR. DNA/ primer mix
The DNA/Primer Mix is made of 50 micro liters of DNA of patients 1 and 2, and primer. There are 2 vials containing negative and positive control. The negative control does not have cancer DNA, and the positive control has cancer in it.
Research and DevelopmentSpecific Cancer Marker Detection - The Underlying Technology (Add a write-up of the information discussed in Week 3's class) (BONUS points: Use a program like Powerpoint, Word, Illustrator, Microsoft Paint, etc. to illustrate how primers bind to the cancer DNA template, and how Taq polymerases amplify the DNA. Screen-captures from the OpenPCR tutorial might be useful. Be sure to credit the source if you borrow images.)
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