BME103 s2013:T900 Group2 L2: Difference between revisions
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'''Placing Samples onto the Fluorimeter''' | '''Placing Samples onto the Fluorimeter''' | ||
* '' | * '''''Step 1:''' A micro pipette was obtained and 80uL of the SYBR GREEN I was dispensed in the center of the first two rows of the slide. Once done 80uL of the desired solution (calf thymus at various concentrations or just water) was added to the mixture on the slide.'' | ||
* '' | * '''''Step two:''' After the desired mixture was placed onto the slide it was then positioned so that the droplet was centered with the small opening on the apparatus where the blue LED light originates from.'' | ||
* ''[Step three, in your own words]'' | * ''[Step three, in your own words]'' | ||
* ''[etc.]'' | * ''[etc.]'' |
Revision as of 15:42, 1 April 2013
BME 103 Spring 2013 | Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help | ||||||||||||||||||||||||||||||||||||||||||||||||
OUR TEAM
LAB 2 WRITE-UPBackground InformationSYBR Green Dye
ProcedureSmart Phone Camera Settings
[Describe how to set up your camera in front of the fluorimeter. Add a PHOTO of this set-up for bonus points.]
[See worksheet page 5]
Placing Samples onto the Fluorimeter
Data AnalysisRepresentative Images of Samples [Show an IMAGE where you drew a circle around the droplet with the freehand tool for a sample with no DNA] [Show an IMAGE where you drew a circle around the droplet with the freehand tool for a sample with DNA (positive signal)]
Image J Values for All Samples [See worksheet page 5]
[Add more rows as needed]
Fitting a Straight Line
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