BME103:W930 Group8 l2: Difference between revisions
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'''Heat Sink/Fan''':<br> | '''Heat Sink/Fan''':<br> | ||
The heat sink and fan will remain the same, but due to the larger PCR block the heat sink and fan will be moved forward slightly(towards the LCD Screen). This simple change will | The heat sink and fan will remain the same, but due to the larger PCR block the heat sink and fan will be moved forward slightly(towards the LCD Screen). This simple change will require no extra work from what was done before, due to the fact that the heat sink and fan attached to the face plate by the PCR block. <br> | ||
'''LCD Screen:'''<br> | '''LCD Screen:'''<br> | ||
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Insulation Pad<br> | Insulation Pad<br> | ||
heated lid handle<br> | heated lid handle<br> | ||
Heated lid mounting plate<br> | |||
40 sample PCR Block<br> | |||
5 mm diameter screws(8)<br> | |||
5 mm diameter washers (8)<br> | |||
thermal pad<br> | |||
'''1'''.Snap one of the pieces into the top piece<br> | '''1'''.Snap one of the pieces into the top piece<br> | ||
'''2'''.Slide the metal nut into the cross section <br> | '''2'''.Slide the metal nut into the cross section <br> | ||
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'''16'''.Screw heated lid handle into top of the lid<br> | '''16'''.Screw heated lid handle into top of the lid<br> | ||
'''17'''.Attach hinge on back of the new and improved heated lid to the face plate using black metal 8mm screws<br> | '''17'''.Attach hinge on back of the new and improved heated lid to the face plate using black metal 8mm screws<br> | ||
'''18'''.Attach enlarged thermal pad to 40 sample PCR block<br> | |||
'''19'''.Attach the following parts from top to bottom in the order as follows: 40 sample PCR Block - Thermal pad - peltier - adapter plate - thermal pad - heat sink.<br> | |||
'''20'''. Mount the PCR Block to the enlarge mounting plate using eight(instead of four as before) 5mm diameter screws and washers<br> | |||
Heat Sink/Fan | Heat Sink/Fan<br> | ||
The heat sink and fan assembly is exaclty the same as before, just moved slightly forward. There is no change required due to the fact that the heat sink and fan attach to the face plate through the PCR Block. <br> | |||
LCD Screen | LCD Screen/PCR Board<br> | ||
The assembly for the LCD Screen and PCR Board is exactly the same as before. The only change is when mounting the LCD Screen and PCR Board it no longer mounts on the top, but instead through the new slot in the front panel. It still mounts using the 16 mm long black screws (4)and Metal nuts (4).<br> | |||
Revision as of 00:05, 28 November 2012
BME 103 Fall 2012 | Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help | |||||||||||||||||||||||||||||||
OUR TEAMLAB 2 WRITE-UPThermal Cycler EngineeringOur re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski.
Key Features Heated Lid/PCR Block: Heat Sink/Fan: LCD Screen: Instructions 1.Snap one of the pieces into the top piece Heat Sink/Fan LCD Screen/PCR Board
ProtocolsMaterials
Research and DevelopmentBackground on Disease Markers
There are many different SNP's for the Prostate Cancer Gene. This is shown in OMIM database reference number 176807. The sequence for this phenotype is: The specific disease name for this SNP is sporadic prostate cancer. It is located on the 22nd chromosome with the Gene ID CHEK 2. The allele change is a G to a A in the positions 614 or 743. This change in the allele leads to an argine to histidine protein residues. This leads to an early onset prostate cancer.
For our first gene dealing with Alzheimer's, the primer design would be:
The second primer design would be: Forward Primer Reverse Primer Both are within the accepted bp primer length (18-22), follows the GC clamp rule (G or C within 5 bp of 3' to clamp the primer down), and have an annealing temperature of 61 degrees Celsius forward and 53 degrees Celsius backward. These all show that the primers forward and backward for this strand above would work. The primer also contains the mutation from the DNA sequence. This would be why the PCR product would give show a cancer gene if there was one, due to the cancerous allele being present. If the non-disease allele were present, the primer would not bind and thus would not amplify.
Illustration
Prostate Cancer PCR Illustration
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