BME103:W930 Group5 l2

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Owwnotebook icon.png BME 103 Fall 2012 Home
Lab Write-Up 1
Lab Write-Up 2
Lab Write-Up 3
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Name: Andrea Carpenter
Role(s): Experimental Protocol Planner
Name: Malik McLaurin
Role(s): Open PCR Machine Engineer
Name: Dana McElwain
Role(s): Open PCR Machine Engineer
Name: Chris Anastos
Roles(s): R&D Scientist
Name: Student


Thermal Cycler Engineering

Our re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski.

System Design

Key Features
The following picture is a simple change in the design of the large exterior screw located on the top of the Open PCR lid:




Supplied in Kit

Supplies Amount
Micro Test Tubes ?
Transfer Pipettes ?
PCR Master Mix 6 samples (598.8μL total)
Positive Control Solution* 1 sample (100.0μL***)
Negative Control Solution** 1 sample (100.0μL***)
PCR Machine 1
Fluorimeter 1

(*)Positive control consists of calf thymus DNA
(**)Negative control simply consists of a blank solution of water
(***)Already mixed with PCR master mix

Included in Fluorimeter Package:

Supplies Amount
Smart phone stand 1
LCD Box 1
Light box 1
Sybr green solution 500.0μL

Components of PCR master mix:

DNA Solution Component Amount
Patient’s Template DNA* 0.2μL
10μM forward primer 1.0μL
10μM reverse primer 1.0μL
Promega GoTaq master mix 50.0μL
dH2O 47.8μL
Total 100.0μL

(*)Not actually included in kit, but must be added to the master mix by the user.

Supplied by User

Supplies Amount
Smart Phone with Camera 1
Patient's Template DNA 6 samples (0.2μL each)
External Computer 1
Image J Software 1
Open PCR Software 1
Gloves 1 pair
Lab Coat 1

PCR Protocol

DNA Measurement Protocol

Research and Development

Background on Disease Markers

For this experiment, our group chose to take an in-depth look at acute myeloid leukemia (AML). AML is a type of cancer that begins inside the bone marrow. The immune system of the human body is ultimately affected by AML, as bone marrow helps fight infections. The white blood cells that grow and form in bone marrow are turned into cancerous cells; the cells grow very quickly and sporadically, thus replacing healthy white blood cells. Our reference single nucleotide polymorphism associated with acute myeloid leukemia is rs121912500.

Primer Design