BME103:TEMPwu2: Difference between revisions
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=LAB 2 WRITE-UP= | =LAB 2 WRITE-UP= | ||
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''' | '''System Design'''<br> | ||
<!--- | |||
'''Key Features'''<br> | |||
'''Instructions'''<br> | |||
<!--- From Week 4 exercise ---> | |||
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==Protocols== | ==Protocols== | ||
''' | <!--- Design a new protocol based on your group's new PCR design. Make a step-by-step list of how someone should use your method | ||
Things to consider: | |||
How should the PCR machine be set up? Does it need to be plugged in? | |||
How many samples will fit into a single 2-hour run? | |||
How many replicates should be created per patient? | |||
What should the final volume of the reaction be? | |||
Will signal reading be integrated into the PCR machine or remain separate? | |||
If it is separate, you will need to include instructions on how to use the fluorimeter. | |||
How should the user calculate the about of signal amplified? | |||
etc. | |||
---> | |||
'''Materials''' | |||
<!--- Place your two tables "Supplied in the kit" and "Supplied by User" here ---> | |||
'''PCR Protocol''' | |||
'''DNA Measurement Protocol''' | |||
==Research and Development== | ==Research and Development== | ||
<!--- | <!--- Bonus: explain how Bayesian statistics can be used to assess the reliability of your team's method. Just write the equation using variables that are relevant to your team's new test. You do not need actual numbers ---> | ||
'''Background on Disease Markers''' | |||
<!--- A description of the diseases and their associated SNP's (include the database reference number and web link) ---> | |||
'''Primer Design''' | |||
<!--- Include the sequences of your forward and reverse primers. Explain why a disease allele will give a PCR product and the non-disease allele will not. ---> | |||
'''Illustration''' | |||
<!--- Include an illustration that shows how your system's primers allow specific amplification of the disease-related SNP ---> | |||
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | ||
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Latest revision as of 13:24, 16 November 2012
 BME 103 Fall 2012
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Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help | |||||
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OUR TEAM
LAB 2 WRITE-UPThermal Cycler EngineeringOur re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski.
Key Features
Instructions
ProtocolsMaterials
PCR Protocol
Research and DevelopmentBackground on Disease Markers
Primer Design
Illustration
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