Difference between revisions of "BME103:T930 Group 3 l2"

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(Research and Development)
(Research and Development)
Line 89: Line 89:
 
rs80356547(http://omim.org/entry/314370#0002) <br>
 
rs80356547(http://omim.org/entry/314370#0002) <br>
 
GATGGCGTGGCCAATGCCCTGGACAA [C/T] GTCCATGCCCGTCAGTTTGGAGGCG <br><br>
 
GATGGCGTGGCCAATGCCCTGGACAA [C/T] GTCCATGCCCGTCAGTTTGGAGGCG <br><br>
 
  
 
'''Primer Design'''
 
'''Primer Design'''

Revision as of 14:43, 21 November 2012

Owwnotebook icon.png BME 103 Fall 2012 Home
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Lab Write-Up 1
Lab Write-Up 2
Lab Write-Up 3
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OUR TEAM

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LAB 2 WRITE-UP

Thermal Cycler Engineering

Our re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski.


System Design


Key Features


Instructions





Protocols

Materials


PCR Protocol



DNA Measurement Protocol



Research and Development

Background on Disease Markers


1.) The amyloid Beta precursor protein for Alzheimer's - rs63751263 (http://omim.org/entry/104760#0008)
AGACGGAGGAGATCTCTGAAGTGAAG [A/C] TGGATGCAGAATTCCGACATGACTC

2.) Ubiquitin-like Modifier-activating enzyme for Spinal muscular atrophy - rs80356547(http://omim.org/entry/314370#0002)
GATGGCGTGGCCAATGCCCTGGACAA [C/T] GTCCATGCCCGTCAGTTTGGAGGCG

Primer Design



Illustration