BME103:T930 Group 14 l2

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Owwnotebook icon.png BME 103 Fall 2012 Home
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Lab Write-Up 1
Lab Write-Up 2
Lab Write-Up 3
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OUR TEAM

Name: Jake Lindquist
Protocol Planner
Name: Breanna Pratt
Protocol Planner
Name: Kirsten Jefferys
Open PCR Machine Engineer
Name: Ben Alcorn
Open PCR Machine Engineer
Name: Carlos Duarte
Research and Design Scientist
Name: Bryce DeSimmone
Research and Design Scientist

LAB 2 WRITE-UP

Thermal Cycler Engineering

Our re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski.


System Design


Key Features


Instructions





Protocols

Materials

Supplied in the kit Amount
PCR Assembly 1
Fluorimeter 1
Phone Stand 1
Box 1


Supplied by User Amount
Samples 3 samples per subject
Positive & Negative controls 1 each
Calibrator (Calif.../water blank) 1 each
Enzyme/Primer mix Enough for all the samples
Test tubes 1 for each sample, and control
Pippettes 1 for each sample, control, and calibration solution


PCR Protocol



DNA Measurement Protocol

Research and Development

Background on Disease Markers

jdkl


Primer Design



Illustration