Difference between revisions of "BME103:T130 Group 7 l2"

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(Thermal Cycler Engineering)
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'''System Design'''<br>
 
'''System Design'''<br>
 
+
Correctly positioning the heating plate on the plastic sample containers proved to be difficult. To make this product more user friendly a button would be added to the heating plate. When in contact with the samples the button would signal the user via the LED display that the heating lid was correctly positioned.
 
[[Image:Roof Assembly small.png]]
 
[[Image:Roof Assembly small.png]]
 +
This picture of the roof assembly shows where the button will be added. The blue circle would contain the button that would, when in contact with the plastic containers below, signal to the user that the heating plate was correctly positioned.
 
[[Image:Plate with Button.png]]
 
[[Image:Plate with Button.png]]
 +
This is another image of where the button would be positioned. The relative size is also accurate as it would need to be able to detect a sample anywhere in the heating block.
 
[[Image:Plastic Body Final.png]]
 
[[Image:Plastic Body Final.png]]
 
[[Image:Power Cord Final.png]]
 
[[Image:Power Cord Final.png]]

Revision as of 12:55, 27 November 2012

Owwnotebook icon.png BME 103 Fall 2012 Home
People
Lab Write-Up 1
Lab Write-Up 2
Lab Write-Up 3
Course Logistics For Instructors
Photos
Wiki Editing Help
BME494 Asu logo.png

OUR TEAM

Name: Emily Thompson
Research & Development Scientist
Name: Vivian Benjes
Experimental Protocol Planner/ Data Analyst
Name: Frances Marrett
Experimental Protocol Planner
Name: Chris Glass
Open PCR Machine Engineer
Name: Ryan Frantz
Open PCR Machine Engineer
[1]
Name: Cenric Nigbur
Ghost/ TBD

LAB 2 WRITE-UP

Thermal Cycler Engineering

Our re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski.


System Design
Correctly positioning the heating plate on the plastic sample containers proved to be difficult. To make this product more user friendly a button would be added to the heating plate. When in contact with the samples the button would signal the user via the LED display that the heating lid was correctly positioned. Roof Assembly small.png This picture of the roof assembly shows where the button will be added. The blue circle would contain the button that would, when in contact with the plastic containers below, signal to the user that the heating plate was correctly positioned. Plate with Button.png This is another image of where the button would be positioned. The relative size is also accurate as it would need to be able to detect a sample anywhere in the heating block. Plastic Body Final.png Power Cord Final.png 32 wells final.png

Key Features


Instructions





Protocols

Materials


Supplied in the Kit

Thermal Cycler for PCR reaction
Qiagen Fast Cycling PCR kit (50)
BioRad Real Time PCR Applications Guide


Supplied by User

Sterile gloves
Access to 120V electrical outlet
10 uL pipette
Eppendorf tube block

PCR Protocol



DNA Measurement Protocol

Research and Development

Background on Disease Markers
Alzheimer's disease, a form of dimentia in which brain function is lost gradually over time, is associated with the SNP rs121918396. The sequence associated with Alzheimer's is TAG, while a normal sequence is TGG.



Primer Design



Illustration